Abstract
The aim of the study was to isolate, identify, and analyze the phylogenetic relationship of the secondary metabolite-producing actinobacterial strains isolated from the mangrove ecosystem of Krishna district, Andhra Pradesh. The potent bioactive metabolite-producing strain was isolated and designated as VLK-104. The identification of the strain was carried out by employing the micromorphological, cultural, physiological, and biochemical methods. The antimicrobial efficacy of the strain was evaluated by using four solvents such as chloroform, ethyl acetate, methanol, and acetone. Among the solvents used, ethyl acetate extract exhibited maximum antimicrobial activity, whereas the other solvent extracts showed moderate to minimum activity against the Gram-positive and Gram-negative bacteria and fungi. Phylogenetic analysis of 16S rRNA gene sequence showed that the strain VLK-104 forms a distinct clade within the Nocardiopsis 16S rRNA gene tree and is closely related to Nocardiopsis lucentensis.
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Acknowledgment
The author was thankful to SERB (Science and Engineering Research Board), New Delhi, for providing the financial support for the present study.
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Naragani, K. (2020). Rare Actinobacteria Nocardiopsis lucentensis VLK-104 Isolated from Mangrove Ecosystem of Krishna District, Andhra Pradesh. In: Khasim, S.M., Long, C., Thammasiri, K., Lutken, H. (eds) Medicinal Plants: Biodiversity, Sustainable Utilization and Conservation. Springer, Singapore. https://doi.org/10.1007/978-981-15-1636-8_33
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DOI: https://doi.org/10.1007/978-981-15-1636-8_33
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