Abstract
The success of regenerative medicine strategies depends majorly on the positive interaction of materials with tissues. Scaffolds that are obtained from polysaccharides and proteins are expected to mimic the natural tissue environment and provide a suitable niche for tissue growth and regeneration. In this work, hydrogels composed of blends of gum Arabic and gelatin were cross-linked with EDC and were freeze-dried to obtain gelatin–gum Arabic (GELGA) sponges. GELGA sponge possesses stable and ordered porous structures. We investigated the cytotoxicity of GELGA disks using L929 cells were very much cytofriendly. This novel sponges promoted adhesion, proliferation and increased matrix production by ovine bone marrow-derived stem cells (OVBMSC). Hence, due to sufficient mechanical properties and ECM production potentials, this can be used in future tissue engineering applications. Essential properties and biological results suggest that GELGA sponge may be a potential candidate for cartilage and bone tissue engineering (TE) strategies.
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Acknowledgements
The authors are thankful to The DST SERB project facility SR/SO/HS/81/2010 and the FIST facility DST/FIST-SR/FST/College-213/2014 of TKM College of Arts and Science.
Ethical Approval All applicable international, national and/or institutional guidelines for the care and use of animals were followed.
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Edwin, B.T., Dhanya, H., Nair, P.D., Kassem, M. (2019). In Vitro and In Vivo Evaluation of 1-(3 Dimethylaminopropyl)-3-Ethyl Carbodiimide (EDC) Cross-Linked Gum Arabic–Gelatin Composite as an Ideal Porous Scaffold for Tissue Engineering. In: Bains, P., Sidhu, S., Bahraminasab, M., Prakash, C. (eds) Biomaterials in Orthopaedics and Bone Regeneration . Materials Horizons: From Nature to Nanomaterials. Springer, Singapore. https://doi.org/10.1007/978-981-13-9977-0_9
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