Skip to main content

Quantitative Analysis of Components in Ganoderma

  • Chapter
  • First Online:
Ganoderma and Health

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1181))

Abstract

The components in Ganoderma, including triterpenes, polysaccharides, nucleosides or nucleobases, steroids, and ergothioneine, were quantitatively analyzed. Among them, the quantitative analysis of triterpenes and polysaccharides were recorded in US Pharmacopeia. HPLC-UV and HPLC-MS are the most popular quantitative methods for all of these compounds.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. USP38–NF33 (2014) United Book Press, Baltimore, pp 9033

    Google Scholar 

  2. Gao J, Sato N, Hattori M, Ma C (2013) The simultaneous quantification of Ganoderma acids and alcohols using ultra high-performance liquid chromatography-mass spectrometry in dynamic selected reaction monitoring mode. J Pharm Biomed Anal 74:246–249

    Article  CAS  Google Scholar 

  3. Krystyna S, Janusz S, Renata L, Marek S, Krzysztof S, Kazimierz G, Anna M (2012) Evaluation of polysaccharides content in fruit bodies and their antimicrobial activity of four Ganoderma lucidum (W Curt.: Fr.) P. Karst. strains cultivated on different wood type substrates. Acta Soc Bot Pol 81(1):17–21

    Article  Google Scholar 

  4. Ma Y, He H, Wu J, Wang C, Chao K, Huang Q (2018) Assessment of polysaccharides from mycelia of genus Ganoderma by mid-infrared and near-infrared spectroscopy. Sci Rep 8(1):10

    Article  Google Scholar 

  5. Chen Y, Xie M, Zhang H, Wang Y, Nie S, Li C (2012) Quantification of total polysaccharides and triterpenoids in Ganoderma lucidum and Ganoderma atrum by near infrared spectroscopy and chemometrics. Food Chem 135:268–275

    Article  CAS  Google Scholar 

  6. Zhao J, Zhang X, Li S, Yang F, Wang Y, Ye W (2006) Quality evaluation of Ganoderma through simultaneous determination of nine triterpenes and sterols using pressurized liquid extraction and high performance liquid chromatography. J Sep Sci 29:2609–2615

    Article  CAS  Google Scholar 

  7. Ha D, Loan L, Hung T, Han L, Khoi N, Dung L, Min B, Nguyen N (2015) An improved HPLC-DAD method for quantitative comparisons of triterpenes in Ganoderma lucidum and its five related species originating from Vietnam. Molecules 20:1059–1077

    Article  Google Scholar 

  8. Gao J, Leung K, Wang Y, Lai C, Li S, Hu L, Lu G, Jiang Z, Yu Z (2007) Qualitative and quantitative analyses of nucleosides and nucleobases in Ganoderma spp. by HPLC-DAD-MS. J Pharm Biomed Anal 44:807–811

    Article  CAS  Google Scholar 

  9. Chen Y, Bicker W, Wu J, Xie M, Lindner W (2012) Simultaneous determination of 16 nucleosides and nucleobases by hydrophilic interaction chromatography and its application to the quality evaluation of Ganoderma. J Agric Food Chem 60:4243–4252

    Article  CAS  Google Scholar 

  10. Yang F, Zhang X, Ge L, Tan S (2011) Determination of nucleosides and related compounds in Ganoderma by CE-MS. J China Pharm Univ 42(4):337–341

    CAS  Google Scholar 

  11. Zhao Y, Lei Z, Liu C, Liu D (2016) Determination of ergothioneine in multiple species of fungi by HPLC. ShipinYanjiu Yu Kaifa 37:117–119

    Google Scholar 

  12. Gao J, Nakamura N, Min B, Hirakawa A, Zuo F, Hattori M (2004) Quantitative determination of bitter principles in specimens of Ganoderma lucidum using high-performance liquid chromatography and its application to the evaluation of Ganoderma products. Chem Pharm Bull 52(6):688–695

    Article  CAS  Google Scholar 

  13. Liu W, Zhang J, Han W, Liu Y, Feng J, Tang C, Feng N (2004) One single standard substance for the simultaneous determination of 17 triterpenses in Ganoderma lingzhi and its related species using high-performance liquid chromagraphy. J Chromatogr B 52(6):688–695

    Google Scholar 

  14. Liu J, Kurashikia K, Fukuta A, Kaneko S, Suimic Y, Shimizu K, Kondo R (2012) Quantitative determination of the representative triterpenoids in the extracts of Ganoderma lucidum with different growth stages using high-performance liquid chromatography for evaluation of their 5α-reductase inhibitory properties. Food Chem 133:1034–1038

    Article  CAS  Google Scholar 

  15. Wang X, Yang M, Guan S, Liu R, Xia J, Bi K, Guo D (2006) Quantitative determination of six major triterpenoids in Ganoderma lucidum and related species by high performance liquid chromatography. J Pharm Biomed Anal 41:838–844

    Article  CAS  Google Scholar 

  16. Li B, Liu C, Wang H, Chen R (2007) The study of quantitative analysis of total triterpenes in Ganoderma. Chin J Chin Mater Med 32(12):1234–1236

    CAS  Google Scholar 

  17. Li B, Gu H, Li Y, Chao L, Wang H, Kang J, Wu C, Chen R (2007) Determination of nine triterpenoid acids from Ganoderma lucidum of different producing area by HPLC. Chin J Chin Mater Med 32(12):1234–1236

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jie Kang .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Chen, R., Kang, J. (2019). Quantitative Analysis of Components in Ganoderma. In: Lin, Z., Yang, B. (eds) Ganoderma and Health. Advances in Experimental Medicine and Biology, vol 1181. Springer, Singapore. https://doi.org/10.1007/978-981-13-9867-4_5

Download citation

Publish with us

Policies and ethics