Biological Characterization and Applications

  • Liliang OuyangEmail author
Part of the Springer Theses book series (Springer Theses)


Chapters  4 6 introduce three bioprinting works using bioinks with different crosslinking mechanisms, mainly from the angles of structural printability and cell viability post-printing. This chapter will present further biological characterization and application based on the specific techniques studied before. Specifically, the printed construct using supramolecular bioinks in Chap.  4 exhibits excellent structure fidelity and mechanical properties and allows for cell adhesion, all of which indicate a promising tissue engineering scaffold. The work in Chap.  5 leads to a perfect balance between structural printability and cell viability by using the easy-accessed and biocompatible bioink, gelatin–alginate hybrid formulation. This technique will be used to further investigate the signal pathway activation and embryonic stem cells’ behavior in 3D-bioprinted constructs. The technology developed in Chap.  6 highlights the use of non-viscous bioinks and flexibility in formulation types and building block complexity. Given this, this work will be used to explore the effects of different ink types and other materials cues on cell behavior, such as morphology.


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Copyright information

© Tsinghua University Press, Beijing and Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.Department of Mechanical EngineeringTsinghua UniversityBeijingChina

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