Biological Characterization and Applications
- 281 Downloads
Chapters 4– 6 introduce three bioprinting works using bioinks with different crosslinking mechanisms, mainly from the angles of structural printability and cell viability post-printing. This chapter will present further biological characterization and application based on the specific techniques studied before. Specifically, the printed construct using supramolecular bioinks in Chap. 4 exhibits excellent structure fidelity and mechanical properties and allows for cell adhesion, all of which indicate a promising tissue engineering scaffold. The work in Chap. 5 leads to a perfect balance between structural printability and cell viability by using the easy-accessed and biocompatible bioink, gelatin–alginate hybrid formulation. This technique will be used to further investigate the signal pathway activation and embryonic stem cells’ behavior in 3D-bioprinted constructs. The technology developed in Chap. 6 highlights the use of non-viscous bioinks and flexibility in formulation types and building block complexity. Given this, this work will be used to explore the effects of different ink types and other materials cues on cell behavior, such as morphology.
- 8.Ohnuki Y, Kurosawa H (2013) Effects of hanging drop culture conditions on embryoid body formation and neuronal cell differentiation using mouse embyonic stem cells: optimization of culture conditions for the formation of well-controlled embryoid bodies. J Biosci Bioeng 115(5):571–574CrossRefGoogle Scholar
- 11.Dorsey SM, McGarvey JR, Wang H, Nikou A, Arama L, Koomalsingh KJ, Kondo N, Gorman JH 3rd, Pilla JJ, Gorman RC, Wenk JF, Burdick JA (2015) MRI evaluation of injectable hyaluronic acid-based hydrogel therapy to limit ventricular remodeling after myocardial infarction. Biomaterials 69:65–75CrossRefGoogle Scholar