Advertisement

Biological Characterization and Applications

  • Liliang OuyangEmail author
Chapter
  • 281 Downloads
Part of the Springer Theses book series (Springer Theses)

Abstract

Chapters  4 6 introduce three bioprinting works using bioinks with different crosslinking mechanisms, mainly from the angles of structural printability and cell viability post-printing. This chapter will present further biological characterization and application based on the specific techniques studied before. Specifically, the printed construct using supramolecular bioinks in Chap.  4 exhibits excellent structure fidelity and mechanical properties and allows for cell adhesion, all of which indicate a promising tissue engineering scaffold. The work in Chap.  5 leads to a perfect balance between structural printability and cell viability by using the easy-accessed and biocompatible bioink, gelatin–alginate hybrid formulation. This technique will be used to further investigate the signal pathway activation and embryonic stem cells’ behavior in 3D-bioprinted constructs. The technology developed in Chap.  6 highlights the use of non-viscous bioinks and flexibility in formulation types and building block complexity. Given this, this work will be used to explore the effects of different ink types and other materials cues on cell behavior, such as morphology.

References

  1. 1.
    Ouyang L, Yao R, Chen X, Na J, Sun W (2015) 3D printing of HEK 293FT cell-laden hydrogel into macroporous constructs with high cell viability and normal biological functions. Biofabrication 7(1):015010CrossRefGoogle Scholar
  2. 2.
    Ouyang L, Yao R, Mao S, Chen X, Na J, Sun W (2015) Three-dimensional bioprinting of embryonic stem cells directs high-throughput and highly uniform embryoid body formation. Biofabrication 7(4):044101CrossRefGoogle Scholar
  3. 3.
    Ouyang L, Highley CB, Sun W, Burdick JA (2017) A generalizable strategy for the 3D bioprinting of hydrogels from nonviscous photo-crosslinkable inks. Adv Mater 29(8)CrossRefGoogle Scholar
  4. 4.
    Park J, Cho CH, Parashurama N, Li Y, Berthiaume F, Toner M, Tilles AW, Yarmush ML (2007) Microfabrication-based modulation of embryonic stem cell differentiation. Lab Chip 7(8):1018–1028CrossRefGoogle Scholar
  5. 5.
    Messana JM, Hwang NS, Coburn J, Elisseeff JH, Zhang Z (2008) Size of the embryoid body influences chondrogenesis of mouse embryonic stem cells. J Tissue Eng Regen Med 2(8):499–506CrossRefGoogle Scholar
  6. 6.
    Hwang YS, Chung BG, Ortmann D, Hattori N, Moeller HC, Khademhosseini A (2009) Microwell-mediated control of embryoid body size regulates embryonic stem cell fate via differential expression of WNT5a and WNT11. Proc Natl Acad Sci U S A 106(40):16978–16983CrossRefGoogle Scholar
  7. 7.
    Itskovitz-Eldor J, Schuldiner M, Karsenti D, Eden A, Yanuka O, Amit M, Soreq H, Benvenisty N (2000) Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germ layers. Mol Med 6(2):88–95CrossRefGoogle Scholar
  8. 8.
    Ohnuki Y, Kurosawa H (2013) Effects of hanging drop culture conditions on embryoid body formation and neuronal cell differentiation using mouse embyonic stem cells: optimization of culture conditions for the formation of well-controlled embryoid bodies. J Biosci Bioeng 115(5):571–574CrossRefGoogle Scholar
  9. 9.
    Weeks CA, Newman K, Turner PA, Rodysill B, Hickey RD, Nyberg SL, Janorkar AV (2013) Suspension culture of hepatocyte-derived reporter cells in presence of albumin to form stable three-dimensional spheroids. Biotechnol Bioeng 110(9):2548–2555CrossRefGoogle Scholar
  10. 10.
    Liu HY, Korc M, Lin CC (2018) Biomimetic and enzyme-responsive dynamic hydrogels for studying cell-matrix interactions in pancreatic ductal adenocarcinoma. Biomaterials 160:24–36CrossRefGoogle Scholar
  11. 11.
    Dorsey SM, McGarvey JR, Wang H, Nikou A, Arama L, Koomalsingh KJ, Kondo N, Gorman JH 3rd, Pilla JJ, Gorman RC, Wenk JF, Burdick JA (2015) MRI evaluation of injectable hyaluronic acid-based hydrogel therapy to limit ventricular remodeling after myocardial infarction. Biomaterials 69:65–75CrossRefGoogle Scholar
  12. 12.
    Stowers RS, Allen SC, Suggs LJ (2015) Dynamic phototuning of 3D hydrogel stiffness. Proc Natl Acad Sci U S A 112(7):1953–1958CrossRefGoogle Scholar
  13. 13.
    Khetan S, Guvendiren M, Legant WR, Cohen DM, Chen CS, Burdick JA (2013) Degradation-mediated cellular traction directs stem cell fate in covalently crosslinked three-dimensional hydrogels. Nat Mater 12(5):458–465CrossRefGoogle Scholar

Copyright information

© Tsinghua University Press, Beijing and Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.Department of Mechanical EngineeringTsinghua UniversityBeijingChina

Personalised recommendations