Abstract
This chapter introduces the overview of heavy metal classification, hazard, and the maximum residue levels in the food. Then, the design of haptens for producing heavy metal antibodies was compared in this chapter. The acquired antibodies targeted to different heavy metal have high specificity and sensitivity, which have been characterized by indirect enzyme-linked immunosorbent assay. Then, the pretreatment methods of different food samples were presented for the application of ELISA for detection of heavy metal. The colloidal gold immunochromatographic strips were also described with different detection ranges and cut-off values. Finally, the perspective of immnunoassays for heavy metal analysis was discussed. As an alternative tool for fast detection heavy metal on-site, there are still lack of effective pretreatment method. Therefore, developing a high-efficiency and simple extraction method is the key factor for heavy metal immunoassays.
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Xu, C., Kuang, H., Xu, L. (2019). Heavy Metal Immunoassay in Food. In: Food Immunoassay. Springer, Singapore. https://doi.org/10.1007/978-981-13-9034-0_3
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DOI: https://doi.org/10.1007/978-981-13-9034-0_3
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