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Mycotoxin Immunoassay in Food

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Food Immunoassay

Abstract

This chapter introduces the overview of mycotoxins containing the characteristics, classification, hazard, and the maximum residue levels in the world. Then the synthetic method of special haptens and antigens was depicted in this chapter. The acquired antibodies based on different antigens have the distinct properties in sensitivity and specificity. The sensitivity and cross-reactivities of obtained antibodies were analyzed via the enzyme-linked immunosorbent assay to provide the basis for hapten design. And the pretreatment methods of different typical samples for ELISA method were presented. Then the colloidal gold immunochromatographic strips based on mycotoxins’ antibodies were described briefly with the principle, the detection ranges, and cut-off values. Finally, the perspective of immnunoassays for mycotoxins analysis was discussed. As a kind of natural product, many kinds of mycotoxins are still being discovered. Therefore, to find the effective synthesis methods of antigenic for antibody preparation is the key driving force for mycotoxin immunoassays.

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Correspondence to Chuanlai Xu .

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Xu, C., Kuang, H., Xu, L. (2019). Mycotoxin Immunoassay in Food. In: Food Immunoassay. Springer, Singapore. https://doi.org/10.1007/978-981-13-9034-0_2

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