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Photodynamic Therapy Against Bacterial Biofilm: Role of Reactive Oxygen Species

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Abstract

Microbial infections remain to be one of the main causes of mortality and the culprits behind these infections are biofilm-forming multiple drug-resistant strains of bacteria. Biofilm are the surface attached, three-dimensional structure of heteromorphic microbial communities embedded in self-producing extracellular polymeric substances (EPSs). Biofilm-associated bacteria exhibit several antibiotic-resistance mechanisms; antibiotic penetration, efflux of the antibiotic, and EPS production are the main mechanisms of antibiotic resistance as they deny drug access to the cell interior. Due to the severity of biofilm-related infections, there is an urgent need to explore novel approaches like photodynamic therapy (PDT) to circumvent this increased resistance. PDT employs a nontoxic, light-sensitive dye called photosensitizer (PS), and harmless visible light of appropriate wavelength to match the absorption peak of the PS in presence of oxygen-rich environment produces a phototoxic response. PDT appears the most promising alternative methodology against multidrug resistance and biofilm-related infections.

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Author information

Authors and Affiliations

  1. Medical Microbiology and Molecular Biology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Lama Misba & Asad U. Khan

Authors
  1. Lama Misba
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  2. Asad U. Khan
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Correspondence to Asad U. Khan .

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Editors and Affiliations

  1. Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, West Bengal, India

    Sajal Chakraborti

  2. Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, West Bengal, India

    Tapati Chakraborti

  3. Amity University, Kolkata, West Bengal, India

    Dhrubajyoti Chattopadhyay

  4. National Institute of Immunology, New Delhi, India

    Chandrima Shaha

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Misba, L., Khan, A.U. (2019). Photodynamic Therapy Against Bacterial Biofilm: Role of Reactive Oxygen Species. In: Chakraborti, S., Chakraborti, T., Chattopadhyay, D., Shaha, C. (eds) Oxidative Stress in Microbial Diseases. Springer, Singapore. https://doi.org/10.1007/978-981-13-8763-0_25

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