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CRISPR Applications in Plant Genetic Engineering and Biotechnology

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Plant Biotechnology: Progress in Genomic Era

Abstract

Genetically modified (GM) crops were first introduced for commercial use in 1996 and as of 2017 were grown on 190 million hectares in 26 countries. Even with the potential these crops believed for worldwide food security, GM crops lately were linked with uncorroborated health concerns, environmental safety, ethical issues and restrictions in European countries.

Over the course of recent years, through improvements in the plant research, new methods have been developed allowing for unambiguous mutations with precision. This includes technologies like mega nucleases, TALENs and zinc finger nucleases (ZFN). These genetic editing techniques are complex, difficult to manipulate constructs and costly. The approval of genetic editing has potential for inherent improvement of crops, improved disease tolerance, improved food, energy, and lint (fibre) security across world. Most importantly, the technology has potential of creating new varieties of crop, tastier vegetables and fibre full grains.

Advances in CRISPR/Cas9 radically cut the cost of performing genetic editing trials, easier implementations and permitted for prolonged use of the technology in plant sciences. We describe here assessment on development, use and trials of CRISPR/Cas9 technology and its implication in crops basic research and crop genetic improvement.

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  1. International Pannacea Limited, Gurgaon, Haryana, India

    Vivek Srivastava

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  1. Amity Institute of Biotechnology, Amity University, Gurgaon, Haryana, India

    S. M. Paul Khurana

  2. Department of Biotechnology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, Uttar Pradesh, India

    Rajarshi Kumar Gaur

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Srivastava, V. (2019). CRISPR Applications in Plant Genetic Engineering and Biotechnology. In: Khurana, S., Gaur, R. (eds) Plant Biotechnology: Progress in Genomic Era. Springer, Singapore. https://doi.org/10.1007/978-981-13-8499-8_19

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