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Standardization of Intratracheal Instillation Study of Manufactured Nanomaterials

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In Vivo Inhalation Toxicity Screening Methods for Manufactured Nanomaterials

Abstract

Intratracheal (IT) instillation is a useful method for screening and hazard identification of inhaled materials, including manufactured nanomaterials. However, many variables regarding sample preparation, experimental equipment, and technical procedures influence results from studies involving IT instillation, and a standard procedure has not yet been validated internationally. These drawbacks prevent accurate comparison of hazard information obtained from different test facilities. In this chapter, we summarize representative IT instillation procedure and present acceptable ranges at which various procedural components do not affect the results. Issues requiring consideration prior to experimentation include the preparation of the dose suspension, appropriate vehicle, and delivery device. In addition, practical aspects of the testing procedure including appropriate forms of anesthesia, animal positioning during IT instillation, intubation methodology, and dosing volume, rate, and frequency are addressed, and recommended endpoints for hazard identification are described. Furthermore, an example recommended procedure for reproducible IT instillation is provided. Technical guidance for reproducible procedures, e.g., a Standard Operating Procedure, is pivotal for the standardization of IT instillation studies, and this chapter contributes validated technical information. In conclusion, IT instillation is poised to occupy an important position regarding hazard screening of manufactured nanomaterials.

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Acknowledgements

This work is part of the research program “Development of innovative methodology for safety assessment of industrial nanomaterials” supported by the Ministry of Economy, Trade and Industry (METI) of Japan.

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Authors and Affiliations

  1. Chemicals Evaluation and Research Institute, Japan, CERI Hita, Hita-shi, Oita, Japan

    Toshio Kobayashi, Yutaka Oshima, Yasuhiro Tsubokura, Takakazu Kayashima, Makoto Nakai & Nobuya Imatanaka

  2. Japan Bioassay Research Center, Japan Organization of Occupational Health and Safety, Hadano, Kanagawa, Japan

    Hirokazu Kano, Hideki Senoh, Masaaki Suzuki, Hitomi Kondo & Shoji Fukushima

Authors
  1. Toshio Kobayashi
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  2. Yutaka Oshima
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  3. Yasuhiro Tsubokura
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  4. Takakazu Kayashima
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  5. Makoto Nakai
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  6. Nobuya Imatanaka
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  7. Hirokazu Kano
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  8. Hideki Senoh
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  9. Masaaki Suzuki
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  10. Hitomi Kondo
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  11. Shoji Fukushima
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Corresponding author

Correspondence to Toshio Kobayashi .

Editor information

Editors and Affiliations

  1. Graduate School of Health Management, Keio University, Fujisawa, Kanagawa, Japan

    Toru Takebayashi

  2. Experimental Toxicology and Ecology, BASF SE, Ludwigshafen am Rhein, Germany

    Robert Landsiedel

  3. National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Masashi Gamo

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© 2019 Springer Nature Singapore Pte Ltd.

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Kobayashi, T. et al. (2019). Standardization of Intratracheal Instillation Study of Manufactured Nanomaterials. In: Takebayashi, T., Landsiedel, R., Gamo, M. (eds) In Vivo Inhalation Toxicity Screening Methods for Manufactured Nanomaterials. Current Topics in Environmental Health and Preventive Medicine. Springer, Singapore. https://doi.org/10.1007/978-981-13-8433-2_6

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  • DOI: https://doi.org/10.1007/978-981-13-8433-2_6

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-13-8432-5

  • Online ISBN: 978-981-13-8433-2

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