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Mitochondria in Health and in Sickness pp 269–277Cite as

Application of CRISPR-Cas9 Screening Technologies to Study Mitochondrial Biology in Healthy and Disease States

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1158))

Abstract

Mitochondria play a central role in maintaining normal cellular homeostasis as well as contributing to the pathogenesis of numerous disease states. The advent of CRISPR-Cas9 screening technologies has greatly accelerated the study of mitochondrial biology. In this chapter, we review the various CRISPR-Cas9 screening platforms that are currently available and prior studies that leveraged this technology to identify genes involved in mitochondrial biology in both healthy and disease states. In addition, we discuss the challenges associated with current CRISPR-Cas9 platforms and potential solutions to further enhance this promising technology.

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Author information

Authors and Affiliations

  1. Princess Margaret Cancer Centre, Toronto, ON, Canada

    David Sharon & Steven M. Chan

Authors
  1. David Sharon
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  2. Steven M. Chan
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Corresponding author

Correspondence to Steven M. Chan .

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Editors and Affiliations

  1. Institute of Biochemistry and Clinical Biochemistry, Università Cattolica del Sacro Cuore, Rome, Italy, Fondazione Policlinico Universitario A. Gemelli – IRCCS, Rome, Italy

    Andrea Urbani

  2. Department of Biochemistry, University of Regina, Regina, SK, Canada

    Mohan Babu

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Sharon, D., Chan, S.M. (2019). Application of CRISPR-Cas9 Screening Technologies to Study Mitochondrial Biology in Healthy and Disease States. In: Urbani, A., Babu, M. (eds) Mitochondria in Health and in Sickness. Advances in Experimental Medicine and Biology, vol 1158. Springer, Singapore. https://doi.org/10.1007/978-981-13-8367-0_15

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