Abstract
A main goal of metagenomic analysis is to elucidate comprehensive functions (i.e., the functionome) of entire communities across various environments, but only PCR amplicon analysis of 16S rDNA has been performed in most metagenomic projects so far. One reason for this is that a standard evaluation method for discerning the functional potentials harbored within genomes or metagenomes has not yet been established. To break this deadlock, a new method was developed to infer the potential functionome based on the completion ratio of the individual Kyoto Encyclopedia of Genes and Genomes functional modules. A prototype system of the MAPLE (Metabolic And Physiological potentiaL Evaluator) to automate all processes used in this new method was then launched in December 2013. The MAPLE system was further improved to increase its usability, and the latest version, MAPLE 2.3.1, is now available through a web interface (https://maple.jamstec.go.jp/maple/maple-2.3.1/).
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Acknowledgments
The author thanks Professor S. Goto of the Database Center for Life Science and Professor K. Takemoto of Kyushu Institute of Technology for their great contribution to this work. The author also thanks W. Arai and T. Nakagawa of JAMSTEC, T. Taniguchi of Mitsubishi Research Institute, K. Yoshimura of NEC Ltd., and H. Uehara of Hewlett-Packard Japan Ltd., for their technical assistance. This work was supported in part by KAKENHI Grants-in-Aid for Scientific Research (Nos. 17H00793 and 15KT0039). This work was also supported in part by grants from the Collaborative Research Program of the Institute for Chemical Research, Kyoto University (Nos. #2013-23 and #2014-24), and by a grant from the Cross-ministerial Strategic Innovation Promotion Program.
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Takami, H. (2019). MAPLE Enables Functional Assessment of Microbiota in Various Environments. In: Gojobori, T., Wada, T., Kobayashi, T., Mineta, K. (eds) Marine Metagenomics. Springer, Singapore. https://doi.org/10.1007/978-981-13-8134-8_7
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DOI: https://doi.org/10.1007/978-981-13-8134-8_7
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