Abstract
In this chapter, the entire developmental process is largely divided into five parts. (1) How to prepare large-scale cell chip with a small number of cells? I developed a sealing-film assisted seeding method. This section will describe the concept and quantitative analysis of the seeding method. (2) How to align and combine the cell chip and the drug chip easily? I developed PS-PDMS hybrid chip to accomplish precise alignment and perfect sealing of individual microwells at the same time. Jig system is introduced to make easy-to-use and robust HTS platform. (3) How to load and deliver a large amount of drugs uniformly, even for hydrophobic drugs? In this section, the development process of freeze-drying based drug loading method will be described, and the characteristics of DLPs provided by this method will be analyzed. (4) How to read the barcode accurately? I will describe the concept and performance of the decoding strategy that I have tried. (5) How to guarantee the number of duplicates for all drug candidates? This section will present the cumulative binary distribution model for statistical analysis of the particle code distribution. I investigated the possibility with Monte-Carlo simulation whether partipetting can guarantee enough duplicates for each drug.
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Song, S.W. (2019). System Development. In: One-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays. Springer Theses. Springer, Singapore. https://doi.org/10.1007/978-981-13-8094-5_2
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DOI: https://doi.org/10.1007/978-981-13-8094-5_2
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