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Application of Fluorescence in Studying Therapeutic Enzymes

  • Zhaoshuai WangEmail author
  • Caihong Li
  • Yinan Wei
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1148)

Abstract

Fluorescence spectroscopy is one of the most important techniques in the study of therapeutic enzymes. The fluorescence phenomenon has been discovered and exploited for centuries, while therapeutic enzymes have been used in treatment of disease for only decades. This chapter provides a brief summary of the current applications of fluorescence methods in studying therapeutic enzymes to provide some insights on the selection of proper method tailored to the goal. First a brief introduction about therapeutic enzymes and history of fluorescence were provided, followed by discussions on how fluorescence was applied in the studies. Four popular fluorescence methods are discussed: fluorescence tracing, fluorescence resonance energy transfer (FRET), fluorescence quenching and fluorescence polarization. Selected application of the fluorescence methods in studying therapeutic enzymes are listed, and discussed in details in the following paragraphs.

Keywords

Therapeutic enzyme Fluorescence tracing FRET Fluorescence quenching Fluorescence polarization 

Abbreviations

FRET

Fluorescence resonance energy transfer

MS

Mass spectroscopy

CD

Circular dichroism

FTIR

Fourier-transform infrared spectroscopy

US-FDA

Food and Drug Administration in USA

SCID

Severe combined immunodeficiency disease

mAbs

Monoclonal antibodies

IgG1

Immunoglobulin G1

GFP

Green Fluorescent Protein

GBA

Glucocerebrosidase

ABP

Activity-based probes

MCL

Mantle cell lymphoma

MESK

Microencapsulated streptokinase

FREE SK

Unencapsulated streptokinase

CFP

Cyan fluorescent protein

YFP

Yellow fluorescent protein

Abl

Ableson

Bcr

Break point cluster

ECFP

Enhanced cyan fluorescent protein

HPMA

N-(2-hydroxypropyl) methacrylamide

ADA

Adenosine deaminase

FAM

6-carboxyfluo rescein

MWCNTs

Multi-walled carbon nanotubes

IgE

Immunoglobulin E

FcεRI

The IgE Fc receptor

IFN-γ

Interferon gamma

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.Department of Pharmaceutical ScienceUniversity of KentuckyLexingtonUSA
  2. 2.Department of Educational, School, and Counseling PsychologyUniversity of KentuckyLexingtonUSA
  3. 3.Department of ChemistryUniversity of KentuckyLexingtonUSA

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