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Novel Effects of Phytogenic Bulk Graphene on Germination and Growth of Monocots and Dicots

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Recent Advances in Material Sciences

Part of the book series: Lecture Notes on Multidisciplinary Industrial Engineering ((LNMUINEN))

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Abstract

Recent years have witnessed many breakthroughs in graphene including mass production of this material. Most of the work focused on the synthesis and study of the properties of graphene. Herein, we first reported on a successful procedure for the reduction of graphite using Stevia leaf extract which resulted in two-dimensional carbon atomic crystals, graphene. This phytogenic graphene (PG) was characterized by ultraviolet visible spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR) and dynamic light scattering technique. High-resolution transmission electron microscopy (HRTEM) and atomic force microscopy (AFM) micrographs showed the variations in surface morphology of the formed graphene which has a stable single-layer structure and was significantly water soluble. AFM data reveals two different sizes 24.9 (G1) and 71.7 nm (G2) of PG. The germination percentage was 19% higher with G1 and G2 treatments than control in peanut a dicot plant, whereas in monocotyledonous plants (rice and maize) the G1 treatment exhibited germination percentage of 50% in maize and 100% in rice. Size-dependent behavior of PG was not noticed in the growth and development of dicot plant (peanut), whereas in monocot plants (maize and rice) size-dependent effects were noticed. These results point to the use of carbonaceous materials in agriculture as bio-efficient plant growth promoting agents.

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References

  1. Begum, P., Ikhtiari, R., Fugetsu, B: Graphene phytotoxicity in the seedling stage of cabbage, tomato, red spinach, and lettuce. Carbon 49(12), 3907–3919 (2011)

    Article  CAS  Google Scholar 

  2. Bolotin, K.I., Sikes, K.J., Jiang, Z., Klima, M., Fudenberg, G., Hone, J., Stormer, H.L.: Ultrahigh electron mobility in suspended graphene. Solid State Commun. 146(9), 351–355 (2008)

    Article  CAS  Google Scholar 

  3. Du, X., Skachko, I., Barker, A., Andrei, E.Y.: Approaching ballistic transport in suspended graphene. Nat. Nanotechnol. 3(8), 491–495 (2008)

    Article  CAS  Google Scholar 

  4. Thakur, S., Karak, N.: Green reduction of graphene oxide by aqueous phytoextracts. Carbon 50(14), 5331–5339 (2012)

    Article  CAS  Google Scholar 

  5. Hummers Jr., W.S., Offeman, R.E.: Preparation of graphitic oxide. J. Am. Chem. Soc. 80(6), 1339 (1958)

    Article  CAS  Google Scholar 

  6. Gurunathan, S., Han, J.W., Eppakayala, V., Kim, J.H.: Microbial reduction of graphene oxide by Escherichia coli: a green chemistry approach. Colloids Surf., B 102, 772–777 (2013)

    Article  CAS  Google Scholar 

  7. Prasad, T.N.V.K.V., Kambala, V.S.R., Convery, B, Naidu, R.: Novel phytosynthesis of nanoparticles using indigenous Australian plants. Nat. Proc. (2011). https://doi.org/10.1038/npre.2011.6307.1

  8. Fernandez-Merino, M.J., Guardia, L., Paredes, J.I., Villar-Rodil, S., Solís-Fernández, P., Martínez-Alonso, A., Tascón, J.M.D.: Vitamin C is an ideal substitute for hydrazine in the reduction of graphene oxide suspensions. J. Phys. Chem. C 114(14), 6426–6432 (2010)

    Article  CAS  Google Scholar 

  9. Esfandiar, A., Akhavan, O., Irajizad, A.: Melatonin as a powerful bio-antioxidant for reduction of graphene oxide. J. Mat. Chem. 21(29), 10907–10914 (2011)

    Article  CAS  Google Scholar 

  10. Zhu, C., Guo, S., Fang, Y., Dong, S.: Reducing sugar: new functional molecules for the green synthesis of graphene nanosheets. ACS Nano 4(4), 2429–2437 (2010)

    Article  CAS  Google Scholar 

  11. Wang, Y., Shi, Z.X., Yin, J.: Facile synthesis of soluble graphene via a green reduction of graphene oxide in tea solution and its biocomposites. ACS Appl. Mat. Interfaces 3(4), 1127–1133 (2011)

    Article  CAS  Google Scholar 

  12. Salas, E.C., Sun, Z., Lüttge, A., Tour, J.M.: Reduction of graphene oxide via bacterial respiration. ACS Nano 4(8), 4852–4856 (2010)

    Article  CAS  Google Scholar 

  13. Chen, G.Y., Pang, D.P., Hwang, S.M., Tuan, H.Y., Hu, Y.C.: A graphene-based platform for induced pluripotent stem cells culture and differentiation. Biomat 33(2), 418–427 (2012)

    Article  Google Scholar 

  14. Lee, W.C., Lim, C.H.Y., Shi, H., Tang, L.A., Wang, Y., Lim, C.T., Loh, K.P.: Origin of enhanced stem cell growth and differentiation on graphene and graphene oxide. ACS Nano 5(9), 7334–7341 (2011)

    Article  CAS  Google Scholar 

  15. Lin, S., Reppert, J., Hu, Q., Hudson, J.S., Reid, M.L., Ratnikova, T.A., Ke, P.C.: Uptake, translocation and transmission of carbon nano materials in rice plants. Small 5(10), 1128–1132 (2009)

    CAS  Google Scholar 

  16. Stampoulis, Dimitrios, Sinha, Saion K., White, Jason C.: Assay-dependent phytotoxicity of nanoparticles to plants. Environ. Sci. Technol. 43(24), 9473–9479 (2009)

    Article  CAS  Google Scholar 

  17. Zhang, X., Hu, W., Li, J., Tao, L., Wei, Y.: A comparative study of cellular uptake and cytotoxicity of multi-walled carbon nanotubes, graphene oxide, and nanodiamond. Toxicol. Res. 1(1), 62–68 (2012)

    Article  CAS  Google Scholar 

  18. Akhavan, O., Ghaderi, E.: Toxicity of graphene and graphene oxide nanowalls against bacteria. ACS Nano 4(10), 5731–5736 (2010)

    Article  CAS  Google Scholar 

  19. Rai, V., Acharya, S., Dey, N.: Implications of nanobiosensors in agriculture. J. Biomater. Nanobiotech. 3, 315–324 (2012)

    Article  CAS  Google Scholar 

  20. Khodakovskaya, M.V., de Silva, K., Nedosekin, D.A., Dervishi, E., Biris, A.S., Shashkov, E.V., Zharov, V.P.: Complex genetic, photothermal, and photoacoustic analysis of nanoparticle-plant interactions. Proc. Nat. Acad. Sci. 108(3), 1028–1033 (2011)

    Article  CAS  Google Scholar 

  21. Nel, A., Xia, T., Mädler, L., Li, N.: Toxic potential of materials at the nano level. Science 311(5761), 622–627 (2006)

    Article  CAS  Google Scholar 

  22. Soejarto, D.D., Douglas Kinghorn, A., Farnsworth, N.R.: Potential sweetening agents of plant origin. III. Organoleptic evaluation of Stevia leaf herbarium samples for sweetness. J. Natl. Prod. 45(5), 590–599 (1982)

    Article  CAS  Google Scholar 

  23. Martelli, A., Frattini, C., Chialva, F.: Unusual essential oils with aromatic properties—I. Volatile components of Stevia rebaudiana bertoni. Flavour Fragr. J. 1(1), 3–7 (1985)

    Article  CAS  Google Scholar 

  24. Rajbhandari, A., Roberts, M.F.: The flavonoids of Steviarebaudiana. J. Nat. Prod. 46(2), 194–195 (1983)

    Article  CAS  Google Scholar 

  25. Maguire, J.D.: Speed of germination—aid in selection and evaluation for seedling emergence and vigor. Crop Sci. 2(2), 176–177 (1962)

    Article  Google Scholar 

  26. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol. 24, 1–15 (1949)

    Article  CAS  Google Scholar 

  27. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193(1), 265–275 (1951)

    CAS  Google Scholar 

  28. Jain, P.K., Lee, K.S., El-Sayed, I.H., El-Sayed, M.A.: Calculated absorption and scattering properties of gold nanoparticles of different size, shape, and composition: applications in biological imaging and biomedicine. J Phys. Chem. B 110(14), 7238–7248 (2006)

    Article  CAS  Google Scholar 

  29. Armendariz, V., Herrera, I., Jose-yacaman, M., Troiani, H., Santiago, P., Gardea-Torresdey, J.L.: Size controlled gold nanoparticle formation by Avena sativa biomass: use of plants in nanobiotechnology. J. Nanopart. Res. 6(4), 377–382 (2004)

    Article  CAS  Google Scholar 

  30. William, W.P.: The two photosystems and their interactions. In: Barber, J. (ed.) The Primary Processes of Photosynthesis, pp. 99–147. Elsevier, Amsterdam (1977)

    Google Scholar 

  31. Woolhouse, H.W.: Toxicity and tolerance in the responses of plants to metals. Physiological Plant Ecology III, pp. 245–300. Springer, Berlin (1983)

    Chapter  Google Scholar 

  32. Chaoui, A., Mazhoudi, S., Ghorbal, M.H., El Ferjani, E.: Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaseolus vulgaris L.). Plant Sci. 127(2), 139–147 (1997)

    Article  CAS  Google Scholar 

  33. Rico, M.C., Majumdar, S., Duarte-gardea, M., Peralta-Videa, R.J., Gardea-Torresdey, L.J.: Interaction of nanoparticles with edible plants and their possible implications in the food chain. J. Agric. Food Chem. 59(8), 3485–3498 (2011)

    Article  CAS  Google Scholar 

  34. Ghodake, G., Seo, Y.D., Park, D., Lee, D.S.: Phytotoxicity of carbon nanotubes assessed by Brassica juncea and Phaseolus mungo. J Nanoelectron. Optoelectron. 5(2), 157–160 (2010)

    Article  CAS  Google Scholar 

  35. Jin, H., Heller, D.A., Sharma, R., Strano, M.S.: Size-dependent cellular uptake and expulsion of single-walled carbon nanotubes: single particle tracking and a generic uptake model for nanoparticles. ACS Nano 3(1), 149–158 (2009)

    Article  CAS  Google Scholar 

  36. Lin, C., Fugetsu, B., Su, Y., Watari, F.: Studies on toxicity of multi walled carbon nanotubes on Arabidopsis T87 suspension cells. J. Hazard. Mater. 170(2–3), 578–583 (2009)

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Nanotechnology Laboratory, Institute of Frontier Technology, Regional Agricultural Research Station, Acharya N G Ranga Agricultural University, Tirupati, 517 502, Andhra Pradesh, India

    T. N. V. K. V. Prasad & T. Giridhara Krishna

  2. Biotechnology Program, Faculty of Agro Based Industry, Universiti Malaysia Kelantan, 17600, Campus Jeli, Malaysia

    P. Visweswara Rao

  3. DST-PURSE Centre, S.V. University, Tirupati, 517 502, Andhra Pradesh, India

    Venkata Subbaiah Kotakadi

  4. S.V. Agricultural College, Tirupati, 517 502, Andhra Pradesh, India

    S. Adam, P. Sudhakar, B. Ravindra Reddy & B. Bhaskar

Authors
  1. T. N. V. K. V. Prasad
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  2. S. Adam
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  3. P. Visweswara Rao
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  4. Venkata Subbaiah Kotakadi
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  5. P. Sudhakar
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  6. B. Ravindra Reddy
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  7. B. Bhaskar
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  8. T. Giridhara Krishna
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Corresponding author

Correspondence to T. N. V. K. V. Prasad .

Editor information

Editors and Affiliations

  1. Department of Mechanical Engineering, Lendi Institute of Engineering and Technology, Jonnada, Andhra Pradesh, India

    Satish Pujari

  2. Department of Mechanical Engineering, Lendi Institute of Engineering and Technology, Jonnada, Andhra Pradesh, India

    Satuluri Srikiran

  3. Faculty of Manufacturing Engineering, Universiti Teknikal Malaysia Melaka, Durian Tunggal, Malacca, Malaysia

    Sivarao Subramonian

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Prasad, T.N.V.K.V. et al. (2019). Novel Effects of Phytogenic Bulk Graphene on Germination and Growth of Monocots and Dicots. In: Pujari, S., Srikiran, S., Subramonian, S. (eds) Recent Advances in Material Sciences . Lecture Notes on Multidisciplinary Industrial Engineering. Springer, Singapore. https://doi.org/10.1007/978-981-13-7643-6_40

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