Skip to main content
SpringerLink
Log in
SpringerLink
Log in

HPTLC Fingerprint in Herbal Drug Formulations

  • Chapter
  • First Online:
Herbal Medicine in India

Abstract

High-performance thin-layer chromatography (HPTLC) is a powerful analytical technique which is wonderfully suitable for qualitative and quantitative analytical tasks. HPTLC plays an important role in today’s analytical world, not in competition to HLPC but as a complementary method. Fingerprint analysis approach using HPTLC has become the most potent technique for quality control of herbal medicines because of its simplicity, flexibility and reliability. It can serve as a tool for identification, authentication and quality control of herbal medicines. The development of chromatographic fingerprints plays an important role in the quality control of complex herbal medicines. Phytochemical evaluation is one of the tools for the quality assessment, which include preliminary phytochemical screening, chemo profiling and marker compound analysis using modern analytical techniques. High-performance thin-layer chromatography (HPTLC) has emerged as an important tool for the qualitative, semi-quantitative and quantitative phytochemical analysis of the herbal drugs and formulations which includes developing TLC fingerprinting profiles and estimation of biomarkers. In this chapter, attempts will be made to expand the use of HPTLC and at the same time create interest among prospective researchers in herbal analysis and to focus on the theoretical background of HPTLC and few examples of herbal drugs and formulations analysed by various researchers using HPTLC method.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Abourashed AA, Mossa JS. HPTLC determination of caffeine in stimulant herbal products and power drinks. J Pharm Biomed Anal. 2004;36:617–20.

    Article  CAS  PubMed  Google Scholar 

  • Ahirwal B, Ahirwal D, Ram A. Evaluation of standards and quality control parameters of herbal drugs, Souvenir, recent trends in herbal therapy 2006: 25–29.

    Google Scholar 

  • Alam P. Densitometric HPTLC analysis of 8-gingerol in Zingiber Officinale extract and ginger- containing dietary supplements, teas, and commercial creams. Asian Pac J Trop Biomed. 2013;3(8):634–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Alam P, Alam A, Anwer MK, Alqasoumi SI. Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels. Asian Pac J Trop Biomed. 2014;4(4):262–6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Amit J, Sunil C, Vimal K, Anupam P. Phytosomes: a revolution in herbal drugs. Pharma Rev. 2007:11–3.

    Google Scholar 

  • Andola HC, Purohit VK. High performance thin layer chromatography (HPTLC): a modern analytical tool for biological analysis. Nat Sci. 2010;8(10):58–61.

    Google Scholar 

  • Arup U, Ekman S, Lindblom L, Mattsson JE. High performance thin layer chromatography (HPTLC), an improved technique for screening lichen substances. Lichenologist. 1993;25:61–71.

    Article  Google Scholar 

  • Attimarad M, MA KK, Aldhubaib BE, Harsha S. High-performance thin layer chromatography: a powerful analytical technique in pharmaceutical drug discovery. Pharma Methods. 2011;2(2):71–4.

    Article  Google Scholar 

  • Bagul MS, Rajani M. Phytochemical evaluation of classical formulation: a case study. Indian Drugs. 2005;42:15–9.

    Google Scholar 

  • Bele AA, Khale A. Standardisation of herbal drugs: an overview. Int Res J Pharm. 2011;2(12):56–60.

    Google Scholar 

  • Brainthwaite A, Smith FJ, Stock R, Rice CBF. Chromatographic methods. 5th ed. London: Kluwer Academic Publishers; 1999.

    Book  Google Scholar 

  • Choudhary N, Sekhon BS. An overview of advances in the standardization of herbal drugs. J Pharm Educ Res. 2011;2(2):55–70.

    Google Scholar 

  • Ekka NR, Namdeo KP, Samal PK. Standardization strategies for herbal drugs: an overview. Res J Pharm Tech. 2008;1(4):310–2.

    Google Scholar 

  • Ermer J. Linearity. In: Ermer J, Miller JHMB, editors. Method validation in pharmaceutical analysis. Weinheim: Wiley-VCH; 2005.

    Chapter  Google Scholar 

  • Gantait A, Barman T, Mukherjee PK. Validated method for estimation of curcumin in turmeric powder. Int J Trad Knowled. 2011;10(2):247–50.

    Google Scholar 

  • Garofolo F. Bioanalytical method validation. In: Chan CC, Lam H, Lee YC, Zhang X-U, editors. Analytical method validation and instrument performance verification. Hoboken, NJ: Wiley-Interscience; 2004. p. 105–38.

    Chapter  Google Scholar 

  • International Conference on Harmonisation (ICH) of Technical Requirements for the Registration of Pharmaceuticals for Human Use. Validation of analytical procedures: methodology. Geneva: ICH-Q2B; 1996.

    Google Scholar 

  • International Organization for Standardization. Accuracy (trueness and precision) of measurement methods and results. Geneva: ISO:DIS 5725–1 to 5725–3; 1994.

    Google Scholar 

  • Jaenchen DE, Reich E. Planar chromatography: instrumentation. In: Encyclopedia of separation science. New York: Academic Press; 2000. p. 839–47.

    Chapter  Google Scholar 

  • Jeganathan NS, Kannan K. HPTLC method for estimation of ellagic acid and gallic acid in Triphala churnam formulations. Res J Phytochem. 2008;2(1):1–9.

    Article  CAS  Google Scholar 

  • Jena A, Saha D, Biswal B, Jana SB, Koley A, Sur D, Battacharya A. Pharmacognostic studies of leaves of Pterospermum suberifolium. Int J Res Pharma Biomed Sci. 2011;2(1):2229–3701.

    Google Scholar 

  • Jork H, Funk W. TLC reagents & detection methods – physical & chemical detection methods: fundamentals. Hoboken: Wiley; 1990. p. 15–40.

    Google Scholar 

  • Knapp DR. Handbook of analytical derivatization reactions. Hoboken: Wiley-Interscience; 1979. p. 2–24.. 449-453, 482

    Google Scholar 

  • Koll K, Reich E, Blatter A, Veit M. Validation of standardized high-performance thin-layer chromatographic methods for quality control and stability testing of herbals. J AOAC Int. 2003;86:909–15.

    CAS  PubMed  Google Scholar 

  • Lee YC. Method validation for HPLC analysis of related substances in pharmaceutical products. In: Chan CC, Lam H, Lee YC, Zhang X-U, editors. Analytical method validation and instrument performance verification. Hoboken, NJ: Wiley-Interscience; 2004. p. 27–9.

    Chapter  Google Scholar 

  • Nyiredy S. Planar chromatographic method development using the PRISMA optimization system and flow charts. J Chromatogr Sci. 2002;40:553–63.

    Article  CAS  PubMed  Google Scholar 

  • Patel K, Bhatt C. Development and validation of a HPTLC method for simultaneous densitometric analysis of Glycyrrhetic acid and Solasodine in herbal drug formulation. Int J Curr Pharm Res. 2017;9(4):29–32.

    Article  CAS  Google Scholar 

  • Patel VR, Patel RK. HPTLC method development and validation for quantification of markers of Dhatrinisha churna. Pharmacog J. 2012;4(29):26–9.

    Article  CAS  Google Scholar 

  • Patel R, Patel M, Dubey N, Dubey N. Patel B. HPTLC method development and validation: strategy to minimize methodological failures. J Food Drug Anal. 2012;20(4):794–804.

    Google Scholar 

  • Patra KC, Pareta SK, Harwansh RK, Kumar JK. Traditional approaches towards standardization of herbal medicines -a review. J Pharm SciTechnol. 2010;2(11):372–9.

    CAS  Google Scholar 

  • Pattanaya P, Jena RK, Panda SKHPTLC. Fingerprinting in the standardisation of sulaharan yoga: an Ayurvedic tablet formulation. Int J Pharma Sci Review Res. 2010;3(2):33–6.

    CAS  Google Scholar 

  • Potawale SE, Gabhe SY, Mahadik KR. Quantification of gymnemagenin and β-sitosterol in marketed herbal formulation by validated normal phase HPTLC method. Chromatogr Res Int. 2014:1–6.

    Google Scholar 

  • Renger B. Quantitative planar chromatography as a tool in pharmaceutical analysis. J AOAC Int. 1993;76:7–13.

    CAS  Google Scholar 

  • Renger B. Contemporary thin layer chromatography in pharmaceutical quality control. J AOAC Int. 1998;81:333–9.

    CAS  Google Scholar 

  • Saraswathy A, Rukmani S, Ariyanathan S. Analysis of Hinguvachadi Churna. Indian J Tradit Knowl. 2009;8(3):319–25.

    Google Scholar 

  • Scott RM. The stationary phase in thin layer chromatography. J Chromatogr Sci. 1973;2:129–35.

    Article  Google Scholar 

  • Sethi PD. Quantitative analysis of pharmaceutical formulations, high performance thin layer chromatography. New Delhi: CBS Publishers and Distributors; 1996. p. 1–30.

    Google Scholar 

  • Shah VP, Midha KK, Findlay JWA, Hill AM, Hulse JD, Mc-Gilveray IJ, McKay G. Bioanalytical method validation – a revisit with a decade progress. Pharma Res. 2000;17(12):1551–8.

    Article  CAS  Google Scholar 

  • Sherma J. Basic techniques, materials and apparatus in handbook of thin-layer chromatography. New York: Marcel Dekker; 1991. p. 3–41.

    Google Scholar 

  • Sherma J. Chromatographic methods of analysis: thin layer chromatography. In: Swarbrick J, editor. Encyclopedia of pharmaceutical technology. 3rd ed. Swarbrick: Informa Healthcare; 2007. p. 538–50.

    Google Scholar 

  • Shewiyo DH, Kaaleb E, Rishab PG, Dejaegherc B, Verbekec JS, Heydenc YV. HPTLC methods to assay active ingredients in pharmaceutical formulations: a review of the method development and validation steps. J Pharm Biomedical Anal. 2012;66:11–23.

    Article  CAS  Google Scholar 

  • Shinde DB, Chavan MJ, Wakte PS. HPTLC in herbal drug quantification. In: Srivastava MM, editor. High-performance thin-layer chromatography (HPTLC). Berlin: Springer; 2011. p. 117–21.

    Chapter  Google Scholar 

  • Srivastava MM. High-performance thin-layer chromatography (HPTLC). Berlin: Springer; 2011. p. 32–60.

    Book  Google Scholar 

  • Van der Hyden Y, Nijhuis A, Smayers-Verbeke J, Vandeginste BMG, Massart DL. Guidance for robustness/ruggedness test in method validation. J Pharm Biomed Anal. 2001;24:723–53.

    Article  Google Scholar 

  • Wagner H. Plant drug analysis: a thin layer chromatography atlas. 2nd ed. Berlin: Springer; 1996.

    Book  Google Scholar 

  • Wall PE. Thin-layer chromatography: a modern practical approach. Chromatography: a modern practical journal of scientific and innovative research 1096 approach. Cambridge: Royal Society of Chemistry; 2005. p. 100–50.

    Google Scholar 

  • Wani MS, Parakh SR, Dehghan MHG, Polshettiwar SA, Chopade VV, Chepurwar S. Herbal medicine and its standardization. Pharma Info. 2007;5(6):1–6.

    Google Scholar 

  • Yuwono M, Indrayanto G. Validation of chromatographic methods of analysis. In: Brittain G, editor. Profiles of drugs substances, excipients and related methodology, vol. 32. San Diego: Academic Press Elsevier Inc; 2005. p. 243–58.

    Chapter  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacy, Regional Institute of Paramedical & Nursing Sciences (RIPANS), Aizawl, Mizoram, India

    Tochhawng Lalhriatpuii

Authors
  1. Tochhawng Lalhriatpuii
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Faculty of Pharmaceutical Science, Assam Down Town University, Guwahati, Assam, India

    Saikat Sen

  2. NEF College of Pharmacy, Lokhra Lalganesh Road, Lokhra, Guwahati, Assam, India

    Raja Chakraborty

Rights and permissions

Reprints and permissions

Copyright information

© 2020 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Lalhriatpuii, T. (2020). HPTLC Fingerprint in Herbal Drug Formulations. In: Sen, S., Chakraborty, R. (eds) Herbal Medicine in India. Springer, Singapore. https://doi.org/10.1007/978-981-13-7248-3_22

Download citation

  • DOI: https://doi.org/10.1007/978-981-13-7248-3_22

  • Published:

  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-13-7247-6

  • Online ISBN: 978-981-13-7248-3

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics

Search

Navigation