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Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine pp 173–196Cite as

Nucleic Acid Amplification Strategy-Based Nanopore Sensors

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Abstract

Nanopore sensing has developed into a powerful tool for single-molecule analysis in a rapid, low-cost, and label-free way. Generally, nanopores include biological nanopores , solid-state nanopores, and hybrid nanopores . Over the past two decades, nanopores have been used for a wide range of applications including gene sequencing and detection of various analytes. To improve the sensitivity of the nanopore sensors, signal amplification technologies, including isothermal amplification and thermocycling amplification, have been introduced into the nanopore system, although the reports are still rare. This chapter focuses on the combination of the signal amplification strategies and nanopore technique in the detection of various analytes.

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Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Detection Technology for Tumour Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi, 276005, Shandong, People’s Republic of China

    Dongmei Xi & Min Liu

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  1. Dongmei Xi
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  2. Min Liu
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Correspondence to Dongmei Xi or Min Liu .

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  1. Shandong Provincial Key Laboratory of Detection Technology for Tumour Markers, Linyi University, Linyi, Shandong, China

    Shusheng Zhang

  2. College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, Shandong, China

    Sai Bi

  3. Shandong Provincial Key Laboratory of Detection Technology for Tumour Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi, Shandong, China

    Xinyue Song

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Xi, D., Liu, M. (2019). Nucleic Acid Amplification Strategy-Based Nanopore Sensors. In: Zhang, S., Bi, S., Song, X. (eds) Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine. Springer, Singapore. https://doi.org/10.1007/978-981-13-7044-1_9

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