Skip to main content
SpringerLink
Log in

Fluorescence Techniques Based on Nucleic Acid Amplification Strategies: Rational Design and Application

  • Chapter
  • First Online:
Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine

  • 756 Accesses

Abstract

Modern fluorescence detection technology plays an important role in the current bioanalysis and clinical detection with merits of the high sensitivity and accuracy. The technology of nucleic acids amplification opens up avenues to establish sensitive fluorescence detection technology. In this chapter, we described the construction of the newly developed fluorescent nano-biosensors based on nucleic acid amplification strategy and provided insightful views on: (1) fluorescence dyes and nanomaterials to develop sensitive and feasible nucleic acid amplification-based fluorescence detection methods; (2) the rational design and application of fluorescence biosensor based on nucleic acid amplification strategies; (3) fluorescence collection and commercial instruments based on nucleic acid amplification strategies.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Adams FC, Barbante C (2013) Nanoscience, nanotechnology and spectrometry. Spectrochim Acta B 86:3–13

    Article  CAS  Google Scholar 

  • Ahmed M, Guleria A, Rath MC et al (2014) Facile and green synthesis of CdSe quantum dots in protein matrix: tuning of morphology and optical properties. J Nanosci Nanotechnol 14:5730–5742

    Article  CAS  PubMed  Google Scholar 

  • Aigner D, Freunberger SA, Wilkening M et al (2014) Enhancing photoinduced electron transfer efficiency of fluorescent pH-probes with halogenated phenols. Anal Chem 86:9293–9300

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Alivisatos AP, Gu W, Larabell C (2005) Quantum dots as cellular probes. Annu Rev Biomed Eng 7:55–76

    Article  CAS  PubMed  Google Scholar 

  • Amat-Guerri F, Costela A, Figuera JM et al (1993) Laser action from rhodamine 6G-doped poly (2-hydroxyethyl methacrylate) matrices with different crosslinking degrees. Chem Phys Lett 209:352–356

    Article  CAS  Google Scholar 

  • Andresen D, Von NR, Bier FF (2009) Helicase dependent OnChip-amplification and its use in multiplex pathogen detection. Clin Chim Acta 403:244–248

    Article  CAS  PubMed  Google Scholar 

  • Auzel F (2004) Upconversion and anti-Stokes processes with f and d ions in solids. Cheminform 35:139–173

    Article  Google Scholar 

  • Baker ES, Hong JW, Gaylord BS et al (2006) PNA/dsDNA complexes: site specific binding and dsDNA biosensor applications. J Am Chem Soc 128:8484–8492

    Article  CAS  PubMed  Google Scholar 

  • Baker M (2012) Digital PCR hits its stride. Nat Methods 9:541–544

    Article  CAS  Google Scholar 

  • Bao H, Hao N, Yang Y et al (2010) Biosynthesis of biocompatible cadmium telluride quantum dots using yeast cells. Nano Res 3:481–489

    Article  CAS  Google Scholar 

  • Bardea A, Burshtein N, Rudich Y et al (2011) Sensitive detection and identification of DNA and RNA using a patterned capillary tube. Anal Chem 83:9418–9423

    Article  CAS  PubMed  Google Scholar 

  • Beija M, Afonso CA, Martinho JM (2009) Synthesis and applications of Rhodamine derivatives as fluorescent probes. Chem Soc Rev 38:2410–2433

    Article  CAS  PubMed  Google Scholar 

  • Beri RK, Khanna PK (2011) Green synthesis of cadmium selenide nanocrystals: the scope of 1, 2, 3-selendiazoles in the synthesis of magic-size nanocrystals and quantum dots. J Nanosci Nanotechnol 11:5137–5142

    Article  CAS  PubMed  Google Scholar 

  • Bossi M, Fölling J, Belov VN et al (2008) Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species. Nano Lett 8:2463–2468

    Article  CAS  PubMed  Google Scholar 

  • Bridges MA, Mcerlane KM, Kwong E et al (1986) Fluorometric determination of nanogram quantities of protein in small samples: application to calcium-transport adenosine triphosphatase. Clin Chim Acta 157:73–79

    Article  CAS  PubMed  Google Scholar 

  • Cao A, Zhang CY (2013) Real-time detection of transcription factors using target-converted helicase-dependent amplification assay with zero-background signal. Anal Chem 85:2543–2547

    Article  CAS  PubMed  Google Scholar 

  • Chamberlain JS, Gibbs RA, Ranier JE et al (1988) Deletion screening of the duchenne muscular dystrophy locus via multiplex DNA amplification. Nucleic Acids Res 16:11141–11156

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Chen F, Lin Z, Zheng Y et al (2012) Development of an automatic multi-channel ink-jet ejection chemiluminescence system and its application to the determination of horseradish peroxidase. Anal Chim Acta 739:77–82

    Article  CAS  PubMed  Google Scholar 

  • Chen F, Mao S, Zeng H et al (2013) Inkjet nanoinjection for high-thoughput chemiluminescence immunoassay on multicapillary glass plate. Anal Chem 85:7413–7418

    Article  CAS  PubMed  Google Scholar 

  • Chen F, Zhang D, Zhang Q et al (2016) Zero-background helicase-dependent amplification and its application to reliable assay of telomerase activity in cancer cell by eliminating primer-dimer artifacts. Chem Bio Chem 17:1171–1176

    Article  CAS  PubMed  Google Scholar 

  • Chen XN, Livak KJ, Kwok PY (1998) A homogeneous, ligase-mediated DNA diagnostic test. Genome Res 8:549–556

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cheng Y, Du Q, Wang L et al (2012) Fluorescently cationic conjugated polymer as an indicator of ligase chain reaction for sensitive and homogeneous detection of single nucleotide polymorphism. Anal Chem 84:3739–3744

    Article  CAS  PubMed  Google Scholar 

  • Cheng Y, Li Z, Zhang X et al (2008) Homogeneous and label-free fluorescence detection of single-nucleotide polymorphism using target-primed branched rolling circle amplification. Anal Biochem 378:123–126

    Article  CAS  PubMed  Google Scholar 

  • Crannell ZA, Rohrman B, Richardskortum R (2014) Quantification of HIV-1 DNA using real-time recombinase polymerase amplification. Anal Chem 86:5615–5619

    Article  CAS  PubMed  Google Scholar 

  • Demuth C, Spindler KLG, Johansen JS et al (2018) Measuring KRAS mutations in circulating tumor DNA by droplet digital PCR and next-generation sequencing. Transl Oncol 11:1220–1224

    Article  PubMed  PubMed Central  Google Scholar 

  • Didenko VV (2006) Fluorescent energy transfer nucleic acid probes. Methods in Molecular Biology™

    Google Scholar 

  • Dobnik D, Stebih D, Blejec A et al (2016) Multiplex quantification of four DNA targets in one reaction with Bio-Rad droplet digital PCR system for GMO detection. Sci Rep 6:35451

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Dobnik D, Spilsberg B, Bogožalec KA et al (2015) Multiplex quantification of 12 European Union authorized genetically modified maize lines with droplet digital polymerase chain reaction. Anal Chem 87:8218–8226

    Article  CAS  PubMed  Google Scholar 

  • Dong H, Gao W, Yan F et al (2010) Fluorescence resonance energy transfer between quantum dots and graphene oxide for sensing biomolecules. Anal Chem 82:5511–5517

    Article  CAS  PubMed  Google Scholar 

  • Drexhage KH (1976) Fluorescence efficiency of laser dyes. J Res Nat Bur Stand 80A:421

    Article  CAS  Google Scholar 

  • Duan X, Li Z, He F et al (2007) A sensitive and homogeneous SNP detection using cationic conjugated polymers. J Am Chem Soc 129:4154

    Article  CAS  PubMed  Google Scholar 

  • Faltin B, Wadle S, Roth G et al (2012) Mediator probe PCR: a novel approach for detection of real-time PCR based on label-free primary probes and standardized secondary universal fluorogenic reporters. Clin Chem 58:1546–1556

    Article  CAS  PubMed  Google Scholar 

  • Feng F, Duan X, Wang S (2009) Fluorescence-amplifying assay for irradiated DNA lesions using water-soluble conjugated polymers. Macromol Rapid Comm 30:147–151

    Article  CAS  Google Scholar 

  • Feng F, He F, An L et al (2008) Fluorescent conjugated polyelectrolytes for biomacromolecule detection. Adv Mater 20:2959–2964

    Article  CAS  Google Scholar 

  • Feng F, Tang Y, He F et al (2010) Cationic conjugated polymer/DNA complexes for amplified fluorescence assays of nucleases and methyltransferases. Adv Mater 19:3490–3495

    Article  CAS  Google Scholar 

  • Gao G, Zhang M, Gong D et al (2017) The size-effect of gold nanoparticles and nanoclusters in the inhibition of amyloid-β fibrillation. Nanoscale 9:4107–4113

    Article  CAS  PubMed  Google Scholar 

  • Gašperšič J, Hafner-Bratkovič I, Stephan M et al (2010) Tetracysteine-tagged prion protein allows discrimination between the native and converted forms. FEBS J 277:2038–2050

    Article  PubMed  CAS  Google Scholar 

  • Gaylord BS, Heeger AJ, Bazan GC (2002) DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes. Proc Natl Acad Sci 99:10954–10957

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ge J, Bai DM, Geng X et al (2018) Fluorometric determination of nucleic acids based on the use of polydopamine nanotubes and target-induced strand displacement amplification. Microchim Acta 185:105

    Article  CAS  Google Scholar 

  • Ge J, Huang ZM, Xi Q et al (2014) A novel graphene oxide based fluorescent nanosensing strategy with hybridization chain reaction signal amplification for highly sensitive biothiol detection. Chem Commun 50:11879–11882

    Article  CAS  Google Scholar 

  • Gonçalves MST (2010) Fluorescent labeling of biomolecules with organic probes. Cheminform 40:190–212

    Google Scholar 

  • Haugland RP, Yguerabide J, Stryer L (1969) Dependence of the kinetics of singlet-singlet energy transfer on spectral overlap. PNAS 63:23–30

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Henegariu O, Heerema NA, Dlouhy SR et al (1997) Multiplex PCR: critical parameters and step-by-step protocol. Biotechniques 23:504–511

    Article  CAS  PubMed  Google Scholar 

  • Heredia NJ, Belgrader P, Wang S et al (2013) Droplet Digital™ PCR quantitation of HER2 expression in FFPE breast cancer samples. Methods 59:S20–S23

    Article  CAS  PubMed  Google Scholar 

  • Higaki T, Liu C, Zeng C et al (2016) Controlling the atomic structure of Au30 nanoclusters by a ligand-based strategy. Angew Chem Int Ed 55:6694–6697

    Article  CAS  Google Scholar 

  • Higuchi R, Dollinger G, Walsh PS et al (1992) Simultaneous amplification and detection of specific DNA sequences. Nat Biotechnol 10:413–417

    Article  CAS  Google Scholar 

  • Higuchi R, Fockler C, Dollinger G et al (1993) Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology 11:1026–1030

    CAS  PubMed  Google Scholar 

  • Hindson BJ, Ness KD, Masquelier DA et al (2011) High-throughput droplet digital PCR system for absolute quantitation of DNA copy number. Anal Chem 83:8604–8610

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ho HA, Boissinot M, Bergeron MG et al (2002) Colorimetric and fluorometric detection of nucleic acids using cationic polythiophene derivatives. Angew Chem Int Ed 41:1548–1551

    Article  CAS  Google Scholar 

  • Ho HA, Najari A, Leclerc M (2008) Optical detection of DNA and proteins with cationic polythiophenes. Acc Chem Res 39:168–178

    Article  CAS  Google Scholar 

  • Holland PM, Abramson RD, Watson R et al (1991) Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of thermus aquaticus DNA polymerase. Proc Natl Acad Sci USA 88:7276–7280

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Huang J, Wu Y, Chen Y et al (2011) Pyrene-excimer probes based on the hybridization chain reaction for the detection of nucleic acids in complex biological fluids. Angew Chem Int Ed 123:421–424

    Article  Google Scholar 

  • Huang K, Yang H, Zhou Z et al (2008) Multisignal chemosensor for Cr3+ and its application in bioimaging. Org Lett 10:2557–2560

    Article  CAS  PubMed  Google Scholar 

  • Hui Y, Wu Z, Qin Z et al (2018) Micro-droplet digital polymerase chain reaction and real-time quantitative polymerase chain reaction technologies provide highly sensitive and accurate detection of Zika Virus. Virol Sin 33:270–277

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Iovannisci DW, Winn-Deen ES (1993) Ligation amplification and fluorescence detection of Mycobacterium tuberculosis DNA. Mol Cell Probe 7:35–43

    Article  CAS  Google Scholar 

  • Joubert MF (1999) Photon avalanche upconversion in rare earth laser materials. Opt Mater 11:181–223

    Article  CAS  Google Scholar 

  • Kongklieng A, Intapan PM, Boonmars T et al (2015) Detection of Babesia canis vogeli and Hepatozoon canis in canine blood by a single-tube real-time fluorescence resonance energy transfer polymerase chain reaction assay and melting curve analysis. J Vet Diagn Invest 27:191–195

    Article  CAS  PubMed  Google Scholar 

  • Lee LG, Connell CR, Bloch W (1993) Allelic discrimination by nick-translation PCR with fluorgenic probes. Nucleic Acids Res 21:3761–3766

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li L, Tian G J. Luo Y et al (2013) Blinking, flickering, and correlation in fluorescence of single colloidal CdSe quantum dots with different shells under different excitations. J Phys Chem C 117:4844–4851

    Article  CAS  Google Scholar 

  • Li L, Tian X, Zou G et al (2008) Quantitative counting of single fluorescent molecules by combined electrochemical adsorption accumulation and total internal reflection fluorescence microscopy. Anal Chem 80:3999–4006

    Article  CAS  PubMed  Google Scholar 

  • Li Z, Miao X, Cheng Z et al (2017) Hybridization chain reaction coupled with the fluorescence quenching of gold nanoparticles for sensitive cancer protein detection. Sens Actuators B Chem 243:731–737

    Article  CAS  Google Scholar 

  • Liu B, Bazan GC (2004) Homogeneous fluorescence-based DNA detection with water soluble conjugated polymers. Chem Mater 16:4467–4476

    Article  CAS  Google Scholar 

  • Liu B, Bazan GC (2005) Methods for strand-specific DNA detection with cationic conjugated polymers suitable for incorporation into DNA chips and microarrays. PNAS 102:589–593

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liu WH, Howarth M, Greytak AB et al (2008) Compact biocompatible quantum dots functionalized for cellular imaging. J Am Chem Soc 130:1274–1284

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liu Y, Luo M, Yan J et al (2013) An ultrasensitive biosensor for DNA detection based on hybridization chain reaction coupled with the efficient quenching of a ruthenium complex to CdTe quantum dots. Chem Commun 49:7424–7426

    Article  CAS  Google Scholar 

  • Liu ZZ, Wang BL, Ma Z et al (2015) Fluorogenic probe for the human ether-a-Go-Go-related gene potassium channel imaging. Anal Chem 87:2550–2554

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Loudet A, Burgess K (2007) BODIPY®dyes and their derivatives: syntheses and spectroscopic properties. Chem Rev 39:4891–4932

    Article  CAS  Google Scholar 

  • Luo J, Shen X, Li B et al (2018) Signal amplification by strand displacement in a carbon dot based fluorometric assay for ATP. Microchim Acta 185:392–400

    Article  CAS  Google Scholar 

  • Ma F, Liu M, Tang B et al (2017) Rapid and sensitive quantification of MicroRNAs by isothermal Helicase-dependent amplification. Anal Chem 89:6182–6197

    Article  CAS  PubMed  Google Scholar 

  • Marras SAE (2006) Selection of fluorophore and quencher pairs for fluorescent nucleic acid hybridization probes. Methods Mol Biol 335:3

    CAS  PubMed  Google Scholar 

  • Martin MM (1975) Hydrogen bond effects on radiationless electronic transitions in xanthene dyes. Chem Phys Lett 35:105–111

    Article  CAS  Google Scholar 

  • Meng HM, Fu T, Zhang XB et al (2012) Efficient fluorescence turn-on probe for zirconium via a target-triggered DNA molecular beacon strategy. Anal Chem 84:2124–2128

    Article  CAS  PubMed  Google Scholar 

  • Meng X, Yang X, Wang K et al (2010) Direct fluorescence detection of point mutations in human genomic DNA using microbead-based ligase chain reaction. Talanta 80:1725–1729

    Article  CAS  PubMed  Google Scholar 

  • Menschikowski M, Jandeck C, Friedemann M et al (2015) Identification and quantification of heterogeneously-methylated DNA fragments using epiallele-sensitive droplet digital polymerase chain reaction (EAST-ddPCR). Epigenetics 10:803–809

    Article  Google Scholar 

  • Morisset D, Štebih D, Milavec M et al (2013) Quantitative analysis of food and feed samples with droplet digital PCR. PLoS ONE 8:e62583

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Navarro E, Serrano-Heras G, CastaãO MJ et al (2015) Real-time PCR detection chemistry. Clin Chim Acta 439:231–250

    Article  CAS  PubMed  Google Scholar 

  • Niu C, Xu Y, Zhang C et al (2018) Ultrasensitive single fluorescence-labeled probe-mediated single universal primer-multiplex-droplet digital polymerase chain reaction for high-throughput genetically modified organism screening. Anal Chem 90:5586–5593

    Article  CAS  PubMed  Google Scholar 

  • Niu S, Jiang Y, Zhang S (2010) Fluorescence detection for DNA using hybridization chain reaction with enzyme-amplification. Chem Commun 46:3089–3091

    Article  CAS  Google Scholar 

  • Niu WB, Wu SL, Zhang SF et al (2011) Multicolor output and shape controlled synthesis of lanthanide-ion doped fluorides upconversion nanoparticles. Dalton Trans 40:3305–3314

    Article  CAS  PubMed  Google Scholar 

  • Parida M, Posadas G, Inoue S et al (2004) Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of west nile virus. J Clin Microbiol 42:257–263

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Parida M, Horioke K, Ishida H et al (2005) Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay. J Clin Microbiol 43:2895–2903

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Prazeres TJV, Fedorov A, Barbosa SP et al (2008) Accurate determination of the limiting anisotropy of rhodamine 101 implications for its use as a fluorescence polarization standard. J Phys Chem A 112:5034

    Article  CAS  PubMed  Google Scholar 

  • Rachlin J, Ding C, Cantor C et al (2005) MuPlex: multi-objective multiplex PCR assay design. Nucleic Acids Res 33:W544

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sameiro T, Goncalves T (2009) Fluorescent labeling of biomolecules with organic probes. Chem Rev 109:190–212

    Article  CAS  Google Scholar 

  • Schietinger S, Menezes LS, Lauritzen B et al (2009) Observation of size dependence in multicolor upconversion in single Yb3+, Er3+ codoped NaYF4 nanocrystals. Nano Lett 9:2477–2481

    Article  CAS  PubMed  Google Scholar 

  • Schuler F, Trotter M, Zengerle R et al (2016) Monochrome multiplexing in PCR by photobleaching of fluorogenic hydrolysis probes. Anal Chem 88:2590–2595

    Article  CAS  PubMed  Google Scholar 

  • Sjöback R, Nygren J, Kubista M (1995) Absorption and fluorescence properties of fluorescein. Spectrochim Acta A 51:L7–L21

    Article  Google Scholar 

  • Su X, Zhu X, Zhang C et al (2012) In situ, real-time monitoring of the 3’ to 5’ exonucleases secreted by living cells. Anal Chem 84:5059–5068

    Article  CAS  PubMed  Google Scholar 

  • Sun WC, Gee KR, Klaubert DH et al (1997) Synthesis of fluorinated fluoresceins. J Org Chem 62:6469–6475

    Article  CAS  Google Scholar 

  • Sun Y, Lu X, Su F et al (2015) Real-time fluorescence ligase chain reaction for sensitive detection of single nucleotide polymorphism based on fluorescence resonance energy transfer. Biosens Bioelectron 74:705–710

    Article  CAS  PubMed  Google Scholar 

  • Talhavini M, Atvars TDZ (1999) Photostability of xanthene molecules trapped in poly(vinyl alcohol) (PVA) matrices. J Photochem Photobiol A 120:141–149

    Article  CAS  Google Scholar 

  • Tao M, Shi Z, Cheng R et al (2015) Highly specific fluorescence detection of T4 polynucleotide kinase activity via photo-induced electron transfer. Anal Biochem 485:18–24

    Article  CAS  PubMed  Google Scholar 

  • Treibs A, Kreuzer FH (1968) Difluorboryl-komplexe von di-und tri-pyrrylmethenen. Justus Liebigs Ann Chem 718:208–223

    Article  CAS  Google Scholar 

  • Tyagi S, Kramer FR (1996) Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol 14:303–308

    Article  CAS  PubMed  Google Scholar 

  • Ueno Y, Jiao GS, Burgess K (2004) Preparation of 5- and 6-carboxyfluorescein. Synth-Stuttg 15:2591–2593

    Google Scholar 

  • Udenfriend S, Stein S, Böhlen P et al (1972) Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range. Science 178:871–872

    Article  CAS  PubMed  Google Scholar 

  • Valasek MA, Repa JJ (2005) The power of real-time PCR. Adv Physiol Educ 29:151–159

    Article  PubMed  Google Scholar 

  • Wang F, Liu XG (2008) Upconversion multicolor fine-tuning: visible to near-infrared emission from lanthanide-doped NaYF4 nanoparticles. J Am Chem Soc 130:5642–5643

    Article  CAS  PubMed  Google Scholar 

  • Weigele M, Debernardo S Leimgruber W (1973) Fluorometric assay of secondary amino acids. Biochem Biophys Res Commun 50:352–356

    Article  CAS  PubMed  Google Scholar 

  • Whale AS, Huggett JF, Cowen S et al (2012) Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation. Nucleic Acids Res 40:e82

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Xu X, Zhang B, Gan P et al (2017) On-nylon membrane detection of nucleic acid molecules by rolling circle amplification. Anal Biochem 533:26–33

    Article  CAS  PubMed  Google Scholar 

  • Xu Y, Zheng Z (2016) Direct RNA detection without nucleic acid purification and PCR: Combining sandwich hybridization with signal amplification based on branched hybridization chain reaction. Biosens Bioelectron 79:593–599

    Article  CAS  PubMed  Google Scholar 

  • Yang L, Liu C, Ren W et al (2012) Graphene surface-anchored fluorescence sensor for sensitive detection of microRNA coupled with enzyme-free signal amplification of hybridization chain reaction. ACS Appl Mater Interfaces 4:6450–6453

    Article  CAS  PubMed  Google Scholar 

  • Yang J, Katagiri D, Mao S et al (2016) Inkjet printing based assembly of thermoresponsive core-shell polymer microcapsules for controlled drug release. J Mater Chem B 4:4156–4163

    Article  CAS  PubMed  Google Scholar 

  • Yao C, Tian S, Liao L et al (2015) Synthesis of fluorescent phenylethanethiolated gold nanoclusters via pseudo-AGR method. Nanoscale 7:16200–16203

    Article  CAS  PubMed  Google Scholar 

  • Yin Y, Alivisatos AP (2005) Colloidal nanocrystal synthesis and the organic-inorganic interface. Nature 437:664–670

    Article  CAS  PubMed  Google Scholar 

  • Yu P, Wen X, Toh YR et al (2015) Fluorescent metallic nanoclusters: electron dynamics, structure, and applications. Part Part Syst Char 32:142–163

    Article  CAS  Google Scholar 

  • Yuan Z, Zhou Y, Gao S et al (2014) Homogeneous and sensitive detection of microRNA with ligase chain reaction and lambda exonuclease-assisted cationic conjugated polymer biosensing. ACS Appl Mater Interfaces 6:6181–6185

    Article  CAS  PubMed  Google Scholar 

  • Zeng H, Yang J, Katagiri D et al (2015) Investigation of monodisperse droplet generation in liquids by inkjet. Sens Actuators B Chem 220:958–961

    Article  CAS  Google Scholar 

  • Zhang JS, Qin WP, Zhao D (2006) Energy transfer processes on both Er3+ ion concentration and excitation densities in Yb3+-Er3+ codoped LaF3 matrix. J Lumin 119:341–345

    Article  CAS  Google Scholar 

  • Zhang W, Li N, Koga D et al (2018) Inkjet printing based droplet generation for integrated online digital polymerase chain reaction. Anal Chem 90:5329–5334

    Article  CAS  PubMed  Google Scholar 

  • Zhang W, Mao S, Yang J et al (2016) The use of an inkjet injection technique in immunoassays by quantitative on-line electrophoretically mediated microanalysis. J Chromatogr A 1477:127–131

    Article  CAS  PubMed  Google Scholar 

  • Zhang Y, Liu J, Zhang CY (2015) Real-time monitoring of small biological molecules by ligation-mediated polymerase chain reaction. Chem Commun 51:12270–12273

    Article  CAS  Google Scholar 

  • Zhao D, Chan WH, He ZK et al (2009) Quantum dot-ruthenium complex dyads: recognition of double-strand DNA through dual-color fluorescence detection. Anal Chem 81:3537–3543

    Article  CAS  PubMed  Google Scholar 

  • Zheng J, Li J, Jiang Y et al (2011) Design of aptamer-based sensing platform using triple-helix molecular switch. Anal Chem 83:6586–6592

    Article  CAS  PubMed  Google Scholar 

  • Zheng W, He L (2009) Label-free, real-time multiplexed DNA detection using fluorescent conjugated polymers. J Am Chem Soc 131:3432–3433

    Article  CAS  PubMed  Google Scholar 

  • Zhou F, Meng R, Liu Q et al (2016) Photoinduced electron transfer-based fluorescence quenching combined with rolling circle amplification for sensitive detection of microRNA. Chemistryselect 1:6422–6428

    Article  CAS  Google Scholar 

  • Zhou J, Liu Z, Liu FY (2012) Upconversion nanophosphors for small-animal imaging. Chem Soc Rev 41:1323–1349

    Article  CAS  PubMed  Google Scholar 

  • Zou L, Li T, Shen R et al (2018) A label-free light-up fluorescent sensing platform based upon hybridization chain reaction amplification and DNA triplex assembly. Talanta 189:137–142

    Article  CAS  PubMed  Google Scholar 

  • Zou Z, Qing Z, He X et al (2014) Ligation-rolling circle amplification combined with Î3-cyclodextrin mediated stemless molecular beacon for sensitive and specific genotyping of single-nucleotide polymorphism. Talanta 125:306–312

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Detection Technology for Tumour Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi, 276005, People’s Republic of China

    Xinyue Song & Yao Jiang

Authors
  1. Xinyue Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yao Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xinyue Song .

Editor information

Editors and Affiliations

  1. Shandong Provincial Key Laboratory of Detection Technology for Tumour Markers, Linyi University, Linyi, Shandong, China

    Shusheng Zhang

  2. College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, Shandong, China

    Sai Bi

  3. Shandong Provincial Key Laboratory of Detection Technology for Tumour Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi, Shandong, China

    Xinyue Song

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Song, X., Jiang, Y. (2019). Fluorescence Techniques Based on Nucleic Acid Amplification Strategies: Rational Design and Application. In: Zhang, S., Bi, S., Song, X. (eds) Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine. Springer, Singapore. https://doi.org/10.1007/978-981-13-7044-1_2

Download citation

Publish with us

Policies and ethics

Search

Navigation