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Nucleic Acid Amplification Strategy-Based Fluorescence Imaging

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Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine

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Abstract

Nucleic acid amplification techniques can be grouped into two major categories, including isothermal amplification and thermocycling amplification. Isothermal amplification techniques include rolling-circle amplification (RCA), strand-displacement amplification (SDA), helicase-dependent amplification (HDA), and hybridization chain reaction (HCR). And polymerase chain reaction (PCR) and ligase chain reaction (LCR) have been known as thermocycling amplification techniques. Now nucleic acid amplification techniques were widely applied in cellular imaging based on fluorescent techniques. In this section, we attempt to summarize recent development of nucleic acid amplification techniques for bio-analysis, including the detection of miRNA, mRNA, ATP, telomerase, pH, and metal ions in cells and biomolecules in cell surface. We also consider the current challenges and our perspectives of nucleic acid amplification techniques for bio-imaging.

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Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Detection Technology for Tumour Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi, 276005, Shandong, People’s Republic of China

    Qiong Li

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  1. Qiong Li
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Correspondence to Qiong Li .

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Editors and Affiliations

  1. Shandong Provincial Key Laboratory of Detection Technology for Tumour Markers, Linyi University, Linyi, Shandong, China

    Shusheng Zhang

  2. College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, Shandong, China

    Sai Bi

  3. Shandong Provincial Key Laboratory of Detection Technology for Tumour Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi, Shandong, China

    Xinyue Song

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Li, Q. (2019). Nucleic Acid Amplification Strategy-Based Fluorescence Imaging. In: Zhang, S., Bi, S., Song, X. (eds) Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine. Springer, Singapore. https://doi.org/10.1007/978-981-13-7044-1_11

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