Abstract
Cyanobacteria (blue-green algae, BGA) are a group of microalgae that plays an essential role in the fixing of atmospheric nitrogen which is important for the soil fertility. BGA can be an economically and ecologically alternative solution for fertilizers in increasing the productivity of Sorghum bicolor. Isolation of these cyanobacteria from natural sources in pure form is an essential step for their efficient use as biofertilizer. The purpose of this study was to investigate cyanobacteria from S. bicolor-grown fields of Ecopark at Cibinong Science Center-Botanic Gardens, Indonesia, as a baseline data. The isolation of pure cultures was done by selecting a single colony from mixed cultures grown on selected media BG-11 and bold basal media, as different cyanobacterial strains can grow on different media. The same medium in liquid form was used for further purification and subculturing. The pure cultures were transferred to liquid media for further studies. From 20 soil sample cultures, 4 predominant isolates were identified on the basis of their morphological characteristics under light microscopy. Observations were made on heterocystous and non-heterocystous forms. The genera of Nostoc and Anabaena were found as the dominant heterocystous group, while the non-heterocystous group consisted of Lyngbya and Oscillatoria in the S. bicolor-grown fields.
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Acknowledgment
This research is supported by the Science and Technology Research Partnership for Sustainable Developments (SATREPS) Project 2016–2021, entitled “Revegetation of Imperata cylindrica field combined with sustainable production and utilization of biomass,” obtained by PKT Kebun Raya-LIPI in cooperation with Satker in Dept. of Life Sciences-LIPI, Pusinov-LIPI, Ministry of Agriculture and Ministry of Forestry, Indonesia.
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Purbani, D.C. (2019). Cyanobacteria from Sorghum bicolor-Grown Fields of Ecopark at Cibinong Science Center-Botanic Gardens, Indonesia. In: Sayyed, R., Reddy, M., Antonius, S. (eds) Plant Growth Promoting Rhizobacteria (PGPR): Prospects for Sustainable Agriculture. Springer, Singapore. https://doi.org/10.1007/978-981-13-6790-8_7
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