Isolated Tissues and Organs

Abstract

Over the years, the use of isolated tissue and/or organ preparations has provided biologists a convenient biological model to work without the systemic influences of the intact animal. The isolated organ/tissue preparations are generally carried out in groups of 2, 4, 8 or more and changes can easily be studied as compared to intact animals. Traditionally, the in vitro dose responses of various drugs are carried out in tissue organ baths by using tissue preparations from various species such as frog, mice, guinea pig, rabbit etc. Different tissue preparations including smooth or skeletal muscles, arterial rings or strips, uterine tissue or vas deferens, ileum, colon, atrial or ventricle, diaphragm etc. are used for these studies. Under controlled environment and perfused with an oxygenated physiological solution condition these experiments are performed.