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Applications of Microfluidic Systems in Biology and Medicine pp 247–274Cite as

In Vitro Tissue Construction for Organ-on-a-Chip Applications

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Part of the book series: Bioanalysis ((BIOANALYSIS,volume 7))

Abstract

Functional living tissues, reconstructed in vitro, have been demanded as grafts for regenerative medicine and drug test models for pharmacokinetic study. For the reconstruction of the functional living tissues, cellular structures with the shapes of point, line and plane are attractive building blocks to form macroscopic cellular tissues. Microfluidics are suitable techniques in the preparation of the cellular structures with design flexibility and high productivity. Owing to their shape controllability, the cellular structures are able to be assembled into macroscopic tissues in various dimensions using microfluidic devices. Furthermore, the integration of in vitro constructed cellular tissues and microfluidic devices provides organ-on-a-chips to evaluate the change of cellular functions in response to applied drugs. This chapter introduces microfluidic fabrication methods for cellular structures with the shapes of point, line and plane, and methods to manipulate and assemble the cellular structures for formation of macroscopic cellular tissues. Furthermore, we show that organ-on-a-chip, combination of the cellular tissues and microfluidic devices, can find applications in biological studies and the analysis of pharmacokinetics and toxicology.

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Authors and Affiliations

  1. Institute of Industrial Science, the University of Tokyo, Tokyo, Japan

    Yuya Morimoto, Nobuhito Mori & Shoji Takeuchi

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  1. Yuya Morimoto
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  2. Nobuhito Mori
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Correspondence to Shoji Takeuchi .

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  1. Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Sapporo, Japan

    Manabu Tokeshi

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Morimoto, Y., Mori, N., Takeuchi, S. (2019). In Vitro Tissue Construction for Organ-on-a-Chip Applications. In: Tokeshi, M. (eds) Applications of Microfluidic Systems in Biology and Medicine . Bioanalysis, vol 7. Springer, Singapore. https://doi.org/10.1007/978-981-13-6229-3_9

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