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Nanopore Device for Single-Molecule Sensing Method and Its Application

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Applications of Microfluidic Systems in Biology and Medicine

Part of the book series: Bioanalysis ((BIOANALYSIS,volume 7))

Abstract

Nanopore analysis is very promising for single-molecule sensing platform. The feature of nanopore platform is a simple, high-throughput single-molecule/particle detection of a wide range of analytes at low-cost at single-molecule level. The single-molecule sensing ability of nanopore device have been utilized for single-molecule DNA sequencing, protein, peptide and carbohydrates detection, and so on. Due to recent progress on the improvement of selectivity, molecular control, fabrication technique, the nanopore platform became “smarter”. Therefore, the applicability of nanopore–sensing methodology are expanding not only for basic research fields but also for medical applications, such as disease diagnosis, drug screening, virus detection.

Herein this chapter, background of nanopore studies (Sect. 11.1), principle of nanopore sensing (Sect. 11.2), the nanopore-fabrication technique (Sect. 11.3), application studies of nanopore sensing (Sect. 11.4), the selective and accuracy improvement studies of nanopore-sensing (Sect. 11.5), recent novel nanopore platform studies (Sect. 11.6), and the summary and future of the nanopore-sensing method (Sect. 11.7) are introduced.

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  1. Osaka University, Ibaraki, Osaka, Japan

    Masateru Taniguchi & Takahito Ohshiro

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  1. Masateru Taniguchi
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Correspondence to Masateru Taniguchi .

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  1. Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Sapporo, Japan

    Manabu Tokeshi

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Taniguchi, M., Ohshiro, T. (2019). Nanopore Device for Single-Molecule Sensing Method and Its Application. In: Tokeshi, M. (eds) Applications of Microfluidic Systems in Biology and Medicine . Bioanalysis, vol 7. Springer, Singapore. https://doi.org/10.1007/978-981-13-6229-3_11

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