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Challenges of Single-Molecule DNA Sequencing with Solid-State Nanopores

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Single Molecule and Single Cell Sequencing

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1129))

Abstract

A powerful DNA sequencing tool with high accuracy, long read length and high-throughput would be required more and more for decoding the complicated genetic code. Solid-state nanopore has attracted many researchers for its promising future as a next-generation DNA sequencing platform due to the processability, the robustness and the large-scale integratability. While the diverse materials have been widely explored for a solid-state nanopore, silicon nitride (Si3N4) is especially preferable from the viewpoint of mass production based on semiconductor fabrication process. Here, as a nanopore sensing mechanism, we focused on the ionic blockade current method which is the most developed technique. We also highlight the main challenges of Si3N4 nanopore-based DNA sequencer that should be addressed: the fabrication of ultra-small nanopore and ultra-thin membrane, the modulation of DNA translocation speed and the detection of base-specific signals. In this chapter, we discuss the recent progress relating to solid-state nanopore DNA sequencing, which helps to provide a comprehensive information about the current technical situation.

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Acknowledgments

The authors would like to express the utmost thanks to all co-workers for their dedication to Hitachi’s solid-state nanopore DNA sequencer project.

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Authors and Affiliations

  1. Center for Technology Innovation – Healthcare, Research & Development Group, Hitachi Ltd., Kokubunji-shi, Tokyo, Japan

    Yusuke Goto, Rena Akahori & Itaru Yanagi

Authors
  1. Yusuke Goto
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  2. Rena Akahori
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  3. Itaru Yanagi
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Corresponding author

Correspondence to Yusuke Goto .

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Editors and Affiliations

  1. Department of Computational Biology and Medical Sciences, University of Tokyo, Chiba, Chiba, Japan

    Yutaka Suzuki

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Goto, Y., Akahori, R., Yanagi, I. (2019). Challenges of Single-Molecule DNA Sequencing with Solid-State Nanopores. In: Suzuki, Y. (eds) Single Molecule and Single Cell Sequencing. Advances in Experimental Medicine and Biology, vol 1129. Springer, Singapore. https://doi.org/10.1007/978-981-13-6037-4_9

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