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Methods in Rhizosphere Biology Research pp 241–253Cite as

Live Imaging of Arbuscular Mycorrhizal Symbiosis

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Part of the book series: Rhizosphere Biology ((RHBIO))

Abstract

Intracellular colonization is essential for the life cycle of arbuscular mycorrhizal fungi (AMF) and the efficiency of plant nutrient uptake. AM functionality is highly associated with the life cycle of intracellular colonization. The colonization cycle is controlled in a cell-autonomous manner. Mycorrhizal roots represent a mosaic of various intracellular colonization stages. Therefore, biochemical studies cannot accurately track the functional dynamics at a cellular level. To better understand the association between colonization dynamics and its functions, a high-resolution spatiotemporal imaging technique is crucial.

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Acknowledgments

This work was supported partly by ACCEL from the Japan Science and Technology Agency (grant number JPMJAC1403).

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Authors and Affiliations

  1. Laboratory of Crop Nutrition, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan

    Yoshihiro Kobae

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  1. Yoshihiro Kobae
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Correspondence to Yoshihiro Kobae .

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Editors and Affiliations

  1. Department of Biology, University of Fribourg, Fribourg, Switzerland

    Didier Reinhardt

  2. Biological Sciences, CBSH, G.B.Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India

    Anil K. Sharma

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Kobae, Y. (2019). Live Imaging of Arbuscular Mycorrhizal Symbiosis. In: Reinhardt, D., Sharma, A. (eds) Methods in Rhizosphere Biology Research. Rhizosphere Biology. Springer, Singapore. https://doi.org/10.1007/978-981-13-5767-1_13

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