Endogenous Signal-Responsive Transgene Switch Systems for Visualization and Purification of Specific Cells
Induced pluripotent stem cells (iPSCs) are induced to differentiate into target cells, and these differentiated cells are used for regenerative medicines or drug discovery. Using endogenous signal-responsive transgene switch systems, scientists can visualize different differentiation states and purify targeted cell types. These systems are composed of transgenes and binding domains of cell-specific proteins or miRNAs to detect endogenous molecules and regulate transgene expression. In this chapter, we describe the designs, operations, and applications of switch systems.
KeywordsRNA binding protein microRNA Synthetic biology Stem cell Differentiation Imaging Cell purification Reporter gene In vitro transcription Regenerative medicine Modified mRNA Translation
We are grateful to Dr. Shunsuke Kawasaki and Mr. Moe Hirosawa for assisting figure preparations. We also thank Dr. Peter Karagiannis (Kyoto University) for English proofreading.