Abstract
Microbial proteases have become more and more attractive in the food industry regarding to its specific properties, such as high production yield, specificity for a certain substrate, and high activity as well as being environmentally friendly. Proteases have also activity in a wide range of temperature (20–80 °C) and pH values (3–13), which increases the fields of application. Chymosin and papain are the well-known proteases and recently novel enzymes and production techniques are studied due to the increase in application areas. Proteases are available in a broad diversity of microorganisms, plants, and animals. However microbial protease productions offer numerous benefits in terms of technical and economic properties such as higher yields in less time and less cost with a higher overall productivity. In this chapter, the studies on microbial protease productions for industrial applications are briefly overviewed. Trend microorganisms and bioreactor configurations are presented together with their potential uses in food industry.
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References
Al-Hagar OE, Ahmed A, Hassan I (2015) Invertase production by irradiated Aspergillus niger OSH5 using agricultural wastes as carbon source. Br Microbiol Res J 6:135–146
Allen C, Stromberg VK, Smith FD, Lacy GH, Mount MS (1986) Complementation of an Erwinia carotovora subsp. carotovora protease mutant with a protease-encoding cosmid. Mol Gen Genet (MGG) 202:276–279
Anbu P (2016) Enhanced production and organic solvent stability of a protease from Brevibacillus laterosporus strain PAP04. Braz J Med Biol Res 49:1–7
Argos P (1987) A sensitive procedure to compare amino acid sequences. J Mol Biol 193:385–396
Berikten D, Kivanc M (2014) Optimization of solid-state fermentation for phytase production by Thermomyces lanuginosus using response surface methodology. Prep Biochem Biotechnol 44:834–848
Blamey JM, Fischer F, Meyer HP, Sarmiento F, Zinn M (2017) Enzymatic biocatalysis in chemical transformations: a promising and emerging field in green chemistry practice. In: Biotechnology of microbial enzymes, 1st edn. Academic Press (an imprint of Elsevier), UK
Dey T, Banerjee R (2012) Hyperactive α-amylase production by Aspergillus oryzae IFO 30103 in a new bioreactor. Lett Appl Microbiol 54:102–107
Dilipkumar M, Rajasimman M, Rajamohan N (2013) Enhanced inulinase production by Streptomyces sp. in solid state fermentation through statistical designs. 3 Biotech 3:509–515
dos Santos Aguilar JG, Sato HH (2017) Microbial proteases: production and application in obtaining protein hydrolysates. Food Res Int 103:253–262
Du Y, Shi P, Huang H, Zhang X, Luo H, Wang Y, Yao B (2013) Characterization of three novel thermophilic xylanases from Humicola insolens Y1 with application potentials in the brewing industry. Biores Technol 130:161–167
Farias M, Valoni E, Castro A, Coelho M (2014) Lipase production by Yarrowia lipolytica in solid state fermentation using different agro industrial residues. Chem Eng Trans 38:301–306
Heredia-Sandoval NG, Valencia-Tapia MY, Calderón de la Barca AM, Islas-Rubio AR (2016) Microbial proteases in baked goods: modification of gluten and effects on immunogenicity and product quality. Foods 5:59–69
Jakovetić SM, Luković ND, Bošković-Vragolović NM, Bezbradica DI, Picazo-Espinosa R, Knežević-Jugović ZD (2013) Comparative study of batch and fluidized bed bioreactors for lipase-catalyzed ethyl cinnamate synthesis. Ind Eng Chem Res 52:16689–16697
Jamrath T, Lindner C, Popović MK, Bajpai R (2012) Production of amylases and proteases by Bacillus caldolyticus from food industry wastes. Food Technol Biotechnol 50:355–361
Kumar CG, Takagi H (1999) Microbial alkaline proteases: from a bioindustrial viewpoint. Biotechnol Adv 17:561–594
Pardo MF, López LM, Canals F, Avilés FX, Natalucci CL, Caffini NO (2000) Purification of balansain I, an endopeptidase from unripe fruits of Bromelia balansae Mez (Bromeliaceae). J Agric Food Chem 48:3795–3800
Parrado J, Rodriguez-Morgado B, Tejada M, Hernandez T, Garcia C (2014) Proteomic analysis of enzyme production by Bacillus licheniformis using different feather wastes as the sole fermentation media. Enzym Microb Technol 57:1–7
Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635
Sanchez S, Demain AL (2017) Useful microbial enzymes—an introduction. In: Biotechnology of microbial enzymes, 1st edn. Academic Press (an imprint of Elsevier), UK
Sawant R, Nagendran S (2014) Protease: an enzyme with multiple industrial applications. World J Pharm Sci 3:568–579
Sheelu G, Kavitha G, Fadnavis NW (2008) Efficient immobilization of lecitase in gelatin hydrogel and degumming of rice bran oil using a spinning basket reactor. J Am Oil Chem Soc 85:739–748
Shehata AN, El Aty A, Abas A (2014) Optimization of process parameters by statistical experimental designs for the production of naringinase enzyme by marine fungi. Int J Chem Eng 2014:1–10
Singh J, Vohra R, Sahoo DK (2004) Enhanced production of alkaline proteases by Bacillus sphaericus using fed-batch culture. Process Biochem 39:1093–1101
Singh R, Mittal A, Kumar M, Mehta PK (2016) Microbial proteases in commercial applications. J Pharm Chem Biol Sci 4:365–374
Souza PM, Bittencourt MLA, Caprara CC, Freitas M, Almeida RPC, Silveira D, Fonseca YM, Ferreira Filho EX, Pessoa Junior A, Magalhães PO (2015) A biotechnology perspective of fungal proteases. Braz J Microbiol 46:337–346
Sun Q, Chen F, Geng F, Luo Y, Gong S, Jiang Z (2018) A novel aspartic protease from Rhizomucor miehei expressed in Pichia pastoris and its application on meat tenderization and preparation of turtle peptides. Food Chem 245:570–577
Veny H, Aroua MK, Sulaiman NMN (2014) Kinetic study of lipase catalyzed transesterification of jatropha oil in circulated batch packed bed reactor. Chem Eng J 237:123–130
Xiao YZ, Wu DK, Zhao SY, Lin WM, Gao XY (2015) Statistical optimization of alkaline protease production from Penicillium citrinum YL-1 under solid-state fermentation. Prep Biochem Biotechnol 45:447–462
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Deniz, I. (2019). Production of Microbial Proteases for Food Industry. In: Parameswaran, B., Varjani, S., Raveendran, S. (eds) Green Bio-processes. Energy, Environment, and Sustainability. Springer, Singapore. https://doi.org/10.1007/978-981-13-3263-0_2
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DOI: https://doi.org/10.1007/978-981-13-3263-0_2
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