Skip to main content

Production of a Transfructosylating Enzymatic Activity Associated to Fructooligosaccharides

  • Chapter
  • First Online:
Book cover Green Bio-processes

Abstract

Biotransformation of sucrose to fructooligosaccharides (FOS) was investigated using the catalytic action of fructosyltransferase (FFase) originated from solid-state fermentation of agro-industrial wastes (sugarcane bagasse, sotol bagasse, Agave fibers, and polyurethane) using four fungal strains (Aspergillus niger GH1, A. niger PSH, Penicillium citrinum, and Penicillium purpurogenum) which have demonstrated ability to produce great diversity of metabolites of industrial interest. Microorganisms and supports were selected based on transfructosylating activity and FOS production. Agave fibers were the best support material since permitted the highest amounts of FOS and FFase, with a FOS productivity of 10.88 g/L * h and yield of 2.70 g/g based on total substrate. Moreover, the At/Ah ratio of FFase was higher for cells cultivated on Agave fibers than those values obtained for the other wastes. Such results showed that Agave fibers can be successfully used as support of A. niger PSH strain for FOS and FTase production.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Bellon-Maurel V, Orliac O, Christen P (2003) Sensors and measurements in solid state fermentation: a review. Process Biochem 38(6):881–896

    Article  CAS  Google Scholar 

  • Buenrostro-Figueroa J, Ascacio-Valdés A, Sepúlveda L et al (2013) Potential use of different agroindustrial by-products as supports for fungal ellagitannase production under solid-state fermentation. Food Bioprod Process

    Google Scholar 

  • Dhake AB, Patil MB (2007) Effect of substrate feeding on production of fructosyltransferase by Penicillium purpurogenum. Braz J Microb 38:194–199

    Article  Google Scholar 

  • Ellaiah P, Adinarayana K, Bhavani Y, Padmaja P, Srinivasulu B (2002) Optimization of process parameters for glucoamylase production under solid state fermentation by a newly isolated Aspergillus species. Process Biochem 38(4):615–620

    Article  CAS  Google Scholar 

  • Fernandez R, Ottoni C, Silva E et al (2007) Screening of β-fructofuranosidase-producing microorganisms and effect of pH and temperature on enzymatic rate. Appl Microbiol Biotechnol 75(1):87–93

    Article  CAS  Google Scholar 

  • Flores-Gallegos AC, Morlett-Chávez J, Aguilar CN, Rodríguez-Herrera R (2012) Inulinase production by a Mexican semi-desert Xerophylic Penicillium citrinum strain under submerged culture. Adv J Food Sci Technol 4(1):46–50

    CAS  Google Scholar 

  • Flores-Maltos A, Rodríguez-Durán LV, Renovato J, Contreras JC, Rodríguez R, Aguilar CN (2011) Catalytical properties of free and immobilized Aspergillus niger Tannase. Enzym Res 2011:6

    Google Scholar 

  • Flores-Maltos DA, Mussatto SI, Contreras-Esquivel JC, Rodríguez-Herrera R, Teixeira JA, Aguilar CN (2014) Biotechnological production and application of fructooligosaccharides. Crit Rev Biotechnol 1–9

    Google Scholar 

  • Ganaie MA, Gupta US, Kango N (2013) Screening of biocatalysts for transformation of sucrose to fructooligosaccharides. J Mol Catal B Enzym 97:12–17

    Article  CAS  Google Scholar 

  • Gervais P, Molin P (2003) The role of water in solid-state fermentation. Biochem Eng J 13(2–3):85–101

    Article  CAS  Google Scholar 

  • Graminha EBN, Gonçalves AZL, Pirota RDPB, Balsalobre MAA, Da Silva R, Gomes E (2008) Enzyme production by solid-state fermentation: application to animal nutrition. Anim Feed Sci Technol 144(1–2):1–22

    Article  CAS  Google Scholar 

  • Hidaka H, Hirayama M, Sumi N (1988) A fructooligosaccharide-producing enzyme from Aspergillus niger ATCC 20611 (biological chemistry). Agric Biol Chem 52(5):1181–1187

    CAS  Google Scholar 

  • La Rotta CE, Ospina SA, López-Munguía A (1998) Producción y Caracterización de Extractos Enzimáticos Crudos con Actividad de Fructosiltransferasa. Revista Colombiana de Ciencias Químico-Farmaceuticas 27:53–56

    Google Scholar 

  • Lateef A, Oloke J, Gueguim-Kana E, Raimi O (2012) Production of fructosyltransferase by a local isolate of Aspergillus niger in both submerged and solid substrate media. Acta Aliment 41(1):100–117

    Article  CAS  Google Scholar 

  • Mussatto SI, Aguilar CN, Rodrigues LR, Teixeira JA (2009) Fructooligosaccharides and β-fructofuranosidase production by Aspergillus japonicus immobilized on lignocellulosic materials. J Mol Catal B Enzym 59(1–3):76–81

    Article  CAS  Google Scholar 

  • Orzua MC, Mussatto SI, Contreras-Esquivel JC et al (2009) Exploitation of agro industrial wastes as immobilization carrier for solid-state fermentation. Ind Crops Prod 30(1):24–27

    Article  CAS  Google Scholar 

  • Patel V, Saunders G, Bucke C (1994) Production of fructooligosaccharides by Fusarium oxysporum. Biotech Lett 16(11):1139–1144

    Article  CAS  Google Scholar 

  • Pérez-Guerra N, Torrado-Agrasar A, López-Macias C, Pastrana L (2003) Main characteristics and applications of solid substrate fermentation. Electron J Environ Agricult Food Chem 2(3)

    Google Scholar 

  • Prapulla SG, Subhaprada V, Karanth NG (2000) Microbial production of oligosaccharides: a review. Adv Appl Microbiol 47:299–343 (Academic Press)

    Google Scholar 

  • Robledo A, Aguilera-Carbó A, Rodriguez R, Martinez J, Garza Y, Aguilar C (2008) Ellagic acid production by Aspergillus niger in solid state fermentation of pomegranate residues. J Ind Microbiol Biotechnol 35(6):507–513

    Article  CAS  Google Scholar 

  • Rodriguez-Duran LV, Contreras-Esquivel JC, Rodriguez R, Prado-Barragan LA, Aguilar CN (2011) Optimization of Tannase production by Aspergillus niger in solid-state packed-bed bioreactor. J Microbiol Biotechnol 21(9):960–967

    Article  CAS  Google Scholar 

  • Sánchez O, Rodriguez A, Silva E, Caicedo L (2010) Sucrose biotransformation to fructooligosaccharides by Aspergillus sp. N74 free cells. Food Bioprocess Technol 3(5):662–673

    Google Scholar 

  • Sangeetha PT, Ramesh MN, Prapulla SG (2005) Maximization of fructooligosaccharide production by two stage continuous process and its scale up. J Food Eng 68(1):57–64

    Article  Google Scholar 

  • Santos AMP, Maugeri F (2007) Synthesis of Fructooligosaccharides from sucrose using inulinase from Kluyveromyces marxianus. Food Technol Biotechnol 45(2):181–186

    CAS  Google Scholar 

  • Veana F, Aguilar C, Herrera RR (2011) Kinetic studies of invertase production by xerophilic Aspergillus and Penicillium strains under submerged culture. Micología Aplicada Internacional 23(2):37–45

    Google Scholar 

  • Yoshikawa J, Amachi S, Shinoyama H, Fujii T (2006) Multiple β-fructofuranosidases by Aureobasidium pullulans DSM2404 and their roles in fructooligosaccharide production. FEMS Microbiol Lett 265(2):159–163

    Article  CAS  Google Scholar 

  • Yoshikawa J, Amachi S, Shinoyama H, Fujii T (2007) Purification and some properties of β-fructofuranosidase I formed by Aureobasidium pullulans DSM 2404. J Biosci Bioeng 103(5):491–493

    Article  CAS  Google Scholar 

  • Yoshikawa J, Amachi S, Shinoyama H, Fujii T (2008) Production of fructooligosaccharides by crude enzyme preparations of β-fructofuranosidase from Aureobasidium pullulans. Biotech Lett 30(3):535–539

    Article  CAS  Google Scholar 

  • Zeng X-A, Zhou K, Liu D et al (2016) Preparation of fructooligosaccharides using Aspergillus niger 6640 whole-cell as catalyst for bio-transformation. LWT Food Sci Technol 65:1072–1079

    Google Scholar 

Download references

Acknowledgements

Authors thank Council of Science and Technology (CONACyT-Mexico) for the financial support given in the frame of the collaborative program among participating institutions.

Conflict of Interest

The authors declare no financial or commercial conflict of interest

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Cristobal N. Aguilar .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Flores-Maltos, A., Mussatto, S.I., Contreras-Esquivel, J.C., Rodríguez, R., Teixeira, J.A., Aguilar, C.N. (2019). Production of a Transfructosylating Enzymatic Activity Associated to Fructooligosaccharides. In: Parameswaran, B., Varjani, S., Raveendran, S. (eds) Green Bio-processes. Energy, Environment, and Sustainability. Springer, Singapore. https://doi.org/10.1007/978-981-13-3263-0_18

Download citation

Publish with us

Policies and ethics