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Cultured Cell Experimental Models

  • Naoto TaniEmail author
  • Tomoya Ikeda
  • Shigeki Oritani
  • Tomomi Michiue
  • Takaki Ishikawa
Chapter
Part of the Current Human Cell Research and Applications book series (CHCRA)

Abstract

A great advantage of cell culture-based studies is the ability to control the physical/chemical environment, including temperature and oxygen partial pressure, as well as the physiological environment, including the presence of molecules such as hormones and metabolites, by manipulating culture medium composition and ambient conditions. Although the pathophysiology of changes of the physiological and physical/chemical environments in culture systems has not been investigated in detail, we believe that a deeper analysis of the ingredients of culture media and the identification of growth factors necessary for cell proliferation (in other words, characterization of the microenvironment for cells, which is mainly based on culture medium) will provide a deeper understanding of cellular functions and cell–cell interactions.

Keywords

Cultured cell Forensic Hypoxia/ischemia Death process Oxygen electrode Dissolved oxygen iPS cell 

Notes

Acknowledgment

This article is a summary of portions from all the references. On completing the article, the author expresses his profound appreciation to all collaborators for their contribution to the parts of the contents.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Naoto Tani
    • 1
    • 2
    Email author
  • Tomoya Ikeda
    • 1
    • 2
  • Shigeki Oritani
    • 1
  • Tomomi Michiue
    • 1
    • 2
  • Takaki Ishikawa
    • 1
    • 2
  1. 1.Department of Legal MedicineOsaka City University Medical SchoolAbenoJapan
  2. 2.Forensic Autopsy SectionMedico-legal Consultation and Postmortem Investigation Support Center (MLCPI-SC)OsakaJapan

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