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Recent Trends and Techniques in Plant Metabolic Engineering pp 47–61Cite as

Current Approaches and Key Applications of Plant Metabolic Engineering

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Abstract

The diversion of carbon flux toward biosynthesis of targeted products could be achieved by manipulation of targeted biosynthesis pathway in plants. This whole process consists of many steps in stepwise manners starting with the identification and isolation of targeted metabolites, elucidation of complete biosynthetic pathway for identification of point of intervention, discovery of corresponding potential metabolic genes, and overexpression of the selected genes in heterologous system and collectively production of the metabolites. The various biochemical processes including transcriptome, translatome, proteome, and reactome are being used to assist metabolic engineering by providing new insights into novel pathways or bottlenecks of existing pathways. Apart from all these, in-depth understanding of metabolic fluxes and feedback regulations is also mandatory for plant metabolic engineering. All these different current approaches are collectively considered for investigating the plant metabolic engineering to understand, reconstruct, analyze, and annotate the targeted pathways. The key applications of plant metabolic engineering have been compiled with a few important applications including improvement of nitrogen utilization in plant, development of highly nutritive food, and generation of biofuel production. In conclusion, the plant metabolic engineering could provide comprehensive evaluation of manipulation of biosynthetic pathways for numerous applications. This compiled information could act as a resource for crop breeding and biotechnology purposes.

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Abbreviations

RBS:

Ribosomal binding site

NNAAs:

Nonnatural amino acids

ORF:

Open reading frame

TAG:

Triacylglycerol

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Authors and Affiliations

  1. Department of Plant Sciences, School for Basic and Applied Sciences, Central University of Punjab (CUPB), Bathinda, Punjab, India

    Bharti,  Reetu & Vinay Kumar

Authors
  1. Bharti
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  2. Reetu
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  3. Vinay Kumar
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Correspondence to Vinay Kumar .

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Editors and Affiliations

  1. Center of Innovative and Applied Bioprocessing (CIAB), Mohali, Punjab, India

    Sudesh Kumar Yadav

  2. Department of Plant Sciences, School for Basic and Applied Sciences, Central University of Punjab (CUPB), Bathinda, Punjab, India

    Vinay Kumar

  3. Center of Innovative and Applied Bioprocessing (CIAB), Mohali, Punjab, India

    Sudhir P Singh

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Bharti, Reetu, Kumar, V. (2018). Current Approaches and Key Applications of Plant Metabolic Engineering. In: Yadav, S., Kumar, V., Singh, S. (eds) Recent Trends and Techniques in Plant Metabolic Engineering. Springer, Singapore. https://doi.org/10.1007/978-981-13-2251-8_3

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