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Identifying Active Methanotrophs and Mitigation of CH4 Emissions in Landfill Cover Soil

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Proceedings of the 8th International Congress on Environmental Geotechnics Volume 2 (ICEG 2018)

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Abstract

In the USA, municipal solid waste (MSW) landfills constitute one of the major anthropogenic sources of methane emissions. In the landfill cover soils employed at MSW landfills, aerobic methane-oxidizing bacteria (MOB) convert CH4 to CO2, thereby partially mitigating the CH4 emissions to the atmosphere. In this study, culture-dependent and culture-independent techniques were employed to evaluate methane oxidation capacity and to characterize the microbial community in landfill cover soil. Microcosms with synthetic landfill gas headspace were used to measure potential methane oxidation rates in landfill cover soil and in methanotrophs-enriched microbial consortia. The results demonstrate that the enriched landfill cover soil supported the growth of a diverse group of methanotrophic and methylotrophic microorganisms, and were dominated by Type I methanotrophs showing positive correlation with CH4 oxidation rates.

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Acknowledgements

This project is funded by the U.S. National Science Foundation (Grant CMMI #1724773), which is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Civil and Materials Engineering, University of Illinois at Chicago, 842 West Taylor Street, Chicago, IL, 60607, USA

    Raksha K. Rai, Jyoti K. Chetri & Krishna R. Reddy

  2. Sequencing Core, Research Resources Center and Department of Biological Sciences, University of Illinois at Chicago, 835 S. Wolcott, Chicago, IL, 60612, USA

    Stefan J. Green

Authors
  1. Raksha K. Rai
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  2. Jyoti K. Chetri
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  3. Stefan J. Green
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  4. Krishna R. Reddy
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Corresponding author

Correspondence to Krishna R. Reddy .

Editor information

Editors and Affiliations

  1. MOE Key Laboratory of Soft Soils and Geoenvironmental Engineering, College of Civil Engineering and Architecture, Zhejiang University, Hangzhou, China

    Liangtong Zhan

  2. MOE Key Laboratory of Soft Soils and Geoenvironmental Engineering, College of Civil Engineering and Architecture, Zhejiang University, Hangzhou, China

    Yunmin Chen

  3. Department of Civil Engineering, Monash University, Clayton, VIC, Australia

    Abdelmalek Bouazza

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Rai, R.K., Chetri, J.K., Green, S.J., Reddy, K.R. (2019). Identifying Active Methanotrophs and Mitigation of CH4 Emissions in Landfill Cover Soil. In: Zhan, L., Chen, Y., Bouazza, A. (eds) Proceedings of the 8th International Congress on Environmental Geotechnics Volume 2. ICEG 2018. Environmental Science and Engineering(). Springer, Singapore. https://doi.org/10.1007/978-981-13-2224-2_38

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