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Integrative Structural Biology with Hybrid Methods pp 59–76Cite as

The PA Tag: A Versatile Peptide Tagging System in the Era of Integrative Structural Biology

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1105))

Abstract

We have recently developed a novel protein tagging system based on the high affinity interaction between an antibody NZ-1 and its antigen PA peptide, a dodecapeptide that forms a β-turn in the binding pocket of NZ-1. This unique conformation allows for the PA peptide to be inserted into turn-forming loops within a folded protein domain and the system has been variously used in general applications including protein purification, Western blotting and flow cytometry, or in more specialized applications such as reporting protein conformational change, and identifying subunits of macromolecular complexes with electron microscopy. Thus the small and “portable” nature of the PA tag system offers a versatile and powerful tool that can be implemented in various aspects of integrative structural biology.

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Authors and Affiliations

  1. Laboratory of Protein Synthesis and Expression, Institute for Protein Research, Osaka University, Suita, Osaka, Japan

    Zuben P. Brown & Junichi Takagi

Authors
  1. Zuben P. Brown
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  2. Junichi Takagi
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Correspondence to Junichi Takagi .

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Editors and Affiliations

  1. Institute for Protein Research, Osaka University, Suita, Osaka, Japan

    Haruki Nakamura

  2. European Bioinformatics Institute, Cambridgeshire, UK

    Gerard Kleywegt

  3. Rutgers, The State University of New Jersey, Piscataway, NJ, USA

    Stephen K. Burley

  4. Biochemistry Department, University of Wisconsin-Madison, Madison, WI, USA

    John L. Markley

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Brown, Z.P., Takagi, J. (2018). The PA Tag: A Versatile Peptide Tagging System in the Era of Integrative Structural Biology. In: Nakamura, H., Kleywegt, G., Burley, S., Markley, J. (eds) Integrative Structural Biology with Hybrid Methods. Advances in Experimental Medicine and Biology, vol 1105. Springer, Singapore. https://doi.org/10.1007/978-981-13-2200-6_6

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