Abstract
Two different fixations are used in FNA cytology: wet fixation by ethanol and air-dried fixation. Wet-fixed smears are mainly stained by Papanicolaou (PAP) staining [1] but some of them also by hematoxylin-eosin (H&E) staining. On the other hand, air-dried smears are stained with May-Grunwald-Giemsa (MGG), Giemsa [2], or Wright staining [3]. MGG stain is also called Pappenheim stain in Germany, where Dr. Pappenheim invented and reported this method in 1911 [4]. MGG stain is one of the Romanowsky stains, which is characterized by Romanowsky effects, i.e., polychromatic effects caused by the mixture of methylene blue, methylene azur, and eosin in the staining solution [5].
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References
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Koshikawa, T., Kyotake, A., Tsukamoto, R., Itoh, T. (2019). Standard and Rapid Stains in Fine-Needle Aspiration Cytology and Rapid On-site Evaluation with A Short Column (Standard and Rapid Stains in Fine-Needle Aspiration Cytology and Rapid On-site Evaluation). In: Kakudo, K. (eds) Thyroid FNA Cytology. Springer, Singapore. https://doi.org/10.1007/978-981-13-1897-9_66
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DOI: https://doi.org/10.1007/978-981-13-1897-9_66
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