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The Hyphenated Technique of High Speed Atomic Force Microscopy and Super Resolution Optical Detection System

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Atomic Force Microscopy in Molecular and Cell Biology

Abstract

The fast scanning AFM combination with super resolution optical technique allow co-localized imaging and manipulation with sub-diffraction resolution in a few seconds. The hybrid technique opens up new fields of in-situ dynamic study in living cells, enzymatic reactions, fibril growth and biomedical research.

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References

  1. Huang P, Andersson SB. High speed atomic force microscopy enabled by a sample profile estimator. Appl Phys Lett. 2013;102:213118.

    Article  Google Scholar 

  2. Kodera N, Yamamoto D, Ishikawa R, Ando T. Video imaging of walking myosin V by high-speed atomic force microscopy. Nature. 2010;468:72–6.

    Article  CAS  Google Scholar 

  3. Casuso I, Khao J, Chami M, Paul-Gilloteaux P, Husain M, Duneau J-P, et al. Characterization of the motion of membrane proteins using high-speed atomic force microscopy. Nat Nanotechnol. 2012;7:525–9.

    Article  CAS  Google Scholar 

  4. Fantner GE, Barbero RJ, Gray DS, Belcher AM. Kinetics of antimicrobial peptide activity measured on individual bacterial cells using high-speed atomic force microscopy. Nat Nanotechnol. 2010;5:280–5.

    Article  CAS  Google Scholar 

  5. Yang C, Yan J, Dukic M, Hosseini N, Zhao J, Fantner GE. Design of a high-bandwidth tripod scanner for high speed atomic force microscopy. Scanning. 2016;38:889–900.

    Article  CAS  Google Scholar 

  6. Weisenburger S, Sandoghdar V. Light microscopy: an ongoing contemporary revolution. Contemp Phys. 2015;56:123–43.

    Article  Google Scholar 

  7. Fernández-Suárez M, Ting AY. Fluorescent probes for super-resolution imaging in living cells. Nat Rev Mol Cell Biol. 2008;9:929–43.

    Article  Google Scholar 

  8. Fantner GE, Schitter G, Kindt JH, Ivanov T, Ivanova K, Patel R, et al. Components for high speed atomic force microscopy. Ultramicroscopy. 2006;106:881–7.

    Article  CAS  Google Scholar 

  9. Kim BI, Boehm RD. Force-feedback high-speed atomic force microscope for studying large biological systems. Micron. 2012;43:1372–9.

    Article  Google Scholar 

  10. Ando T, Uchihashi T, Kodera N. High-speed AFM and applications to biomolecular systems. Annu Rev Biophys. 2013;42:393–414.

    Article  CAS  Google Scholar 

  11. Mikheikin A, Olsen A, Picco L, Payton O, Mishra B, Gimzewski JK, et al. High-speed atomic force microscopy revealing contamination in DNA purification systems. Anal Chem. 2016;88:2527–32.

    Article  CAS  Google Scholar 

  12. Sanchez H, Suzuki Y, Yokokawa M, Takeyasu K, Wyman C. Protein–DNA interactions in high speed AFM: single molecule diffusion analysis of human RAD54. Integr Biol. 2011;3:1127–34.

    Article  CAS  Google Scholar 

  13. Ando T. High-speed atomic force microscopy coming of age. Nanotechnology. 2012;23:062001.

    Article  Google Scholar 

  14. Uchihashi T, Iino R, Ando T, Noji H. High-speed atomic force microscopy reveals rotary catalysis of Rotorless F1-ATPase. Science. 2011;333:755–8.

    Article  CAS  Google Scholar 

  15. Ruan Y, Miyagi A, Wang X, Chami M, Boudker O, Scheuring S. Direct visualization of glutamate transporter elevator mechanism by high-speed AFM. Proc Natl Acad Sci. 2017;114:1584–8.

    Article  CAS  Google Scholar 

  16. Shim S-H, Xia C, Zhong G, Babcock HP, Vaughan JC, Huang B, et al. Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes. Proc Natl Acad Sci. 2012;109:13978–83.

    Article  CAS  Google Scholar 

  17. Suzuki Y, Sakai N, Yoshida A, Uekusa Y, Yagi A, Imaoka Y, et al. High-speed atomic force microscopy combined with inverted optical microscopy for studying cellular events. Sci Rep. 2013;3:2131.

    Article  Google Scholar 

  18. Meister A, Gabi M, Behr P, Studer P, Vörös J, Niedermann P, et al. FluidFM: combining atomic force microscopy and Nanofluidics in a universal liquid delivery system for single cell applications and beyond. Nano Lett. 2009;9:2501–7.

    Article  CAS  Google Scholar 

  19. Uchihashi T, Watanabe H, Fukuda S, Shibata M, Ando T. Functional extension of high-speed AFM for wider biological applications. Ultramicroscopy. 2016;160:182–96.

    Article  CAS  Google Scholar 

  20. Nievergelt AP, Erickson BW, Hosseini N, Adams JD, Fantner GE. Studying biological membranes with extended range high-speed atomic force microscopy. Sci Rep. 2015;5:11987.

    Article  CAS  Google Scholar 

  21. Eghiaian F, Rico F, Colom A, Casuso I, Scheuring S. High-speed atomic force microscopy: imaging and force spectroscopy. FEBS Lett. 2014;588:3631–8.

    Article  CAS  Google Scholar 

  22. Zhou H-T, Gao X, Zheng P, Qin M, Cao Y, Wang W. Mechanical properties of elastomeric proteins studied by single molecule force spectroscopy. Acta Phys Sin. 2016;65:188703.

    Google Scholar 

  23. Endo M, Sugiyama H. Single-molecule imaging of dynamic motions of biomolecules in DNA origami nanostructures using high-speed atomic force microscopy. Acc Chem Res. 2014;47:1645–53.

    Article  CAS  Google Scholar 

  24. Watanabe H, Uchihashi T, Kobashi T, Shibata M, Nishiyama J, Yasuda R, et al. Wide-area scanner for high-speed atomic force microscopy. Rev Sci Instrum. 2013;84:053702.

    Article  Google Scholar 

  25. Shibata M, Uchihashi T, Ando T, Yasuda R. Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells. Sci Rep. 2015;5:8724.

    Article  CAS  Google Scholar 

  26. Igarashi K, Uchihashi T, Uchiyama T, Sugimoto H, Wada M, Suzuki K, et al. Two-way traffic of glycoside hydrolase family 18 processive chitinases on crystalline chitin. Nat Commun. 2014;5:3975.

    Article  CAS  Google Scholar 

  27. Huang B, Bates M, Zhuang X. Super-Resolution Fluorescence Microscopy. Annu Rev Biochem. 2009;78:993–1016.

    Article  CAS  Google Scholar 

  28. Westphal V, Rizzoli SO, Lauterbach MA, Kamin D, Jahn R, Hell SW. Video-rate far-field optical Nanoscopy dissects synaptic vesicle movement. Science. 2008;320:246–9.

    Article  CAS  Google Scholar 

  29. Nägerl UV, Willig KI, Hein B, Hell SW, Bonhoeffer T. Live-cell imaging of dendritic spines by STED microscopy. Proc Natl Acad Sci. 2008;105:18982–7.

    Article  Google Scholar 

  30. Hell SW, Wichmann J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Opt Lett. 1994;19:780–2.

    Article  CAS  Google Scholar 

  31. Klar TA, Jakobs S, Dyba M, Egner A, Hell SW. Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission. Proc Natl Acad Sci. 2000;97:8206–10.

    Article  CAS  Google Scholar 

  32. Tressler C, Stolle M, Fradin C. Fluorescence correlation spectroscopy with a doughnut-shaped excitation profile as a characterization tool in STED microscopy. Opt Express. 2014;22:31154–66.

    Article  Google Scholar 

  33. Harke B, Chacko JV, Haschke H, Canale C, Diaspro A. A novel nanoscopic tool by combining AFM with STED microscopy. Opt Nanosc. 2012;1:3.

    Article  Google Scholar 

  34. Rust MJ, Bates M, Zhuang X. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat Methods. 2006;3:793–6.

    Article  CAS  Google Scholar 

  35. Dickson RM, Cubitt AB, Tsien RY, Moerner WE. On/off blinking and switching behaviour of single molecules of green fluorescent protein. Nature. 1997;388:355.

    Article  CAS  Google Scholar 

  36. Odermatt PD, Shivanandan A, Deschout H, Jankele R, Nievergelt AP, Feletti L, et al. High-resolution correlative microscopy: bridging the gap between single molecule localization microscopy and atomic force microscopy. Nano Lett. 2015;15:4896–904.

    Article  CAS  Google Scholar 

  37. Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, Bonifacino JS, et al. Imaging intracellular fluorescent proteins at nanometer resolution. Science. 2006;313:1642–5.

    Article  CAS  Google Scholar 

  38. Wolter S, Schuttpelz M, Tscherepanow M, van de Linde S, Heilemann M, Sauer M. Real-time computation of subdiffraction-resolution fluorescence images. J Microsc. 2010;237:12–22.

    Article  CAS  Google Scholar 

  39. Hansma HG, Vesenka J, Siegerist C, Kelderman G, Morrett H, Sinsheimer RL, et al. Reproducible imaging and dissection of plasmid DNA under liquid with the atomic force microscope. Science. 1992;256:1180–4.

    Article  CAS  Google Scholar 

  40. Stark RW, Thalhammer S, Wienberg J, Heckl WM. The AFM as a tool for chromosomal dissection – the influence of physical parameters. Appl Phys A Mater Sci Process. 1998;66:S579–S84.

    Article  CAS  Google Scholar 

  41. Fotiadis D, Scheuring S, Müller SA, Engel A, Müller DJ. Imaging and manipulation of biological structures with the AFM. Micron. 2002;33:385–97.

    Article  CAS  Google Scholar 

  42. An H, Guo Y, Zhang X, Zhang Y, Hu J. Nanodissection of single- and double-stranded DNA by atomic force microscopy. J Nanosci Nanotechnol. 2005;5:1656–9.

    Article  Google Scholar 

  43. An H, Huang J, Lü M, Li X, Lü J, Li H, et al. Single-base resolution and long-coverage sequencing based on single-molecule nanomanipulation. Nanotechnology. 2007;18:225101.

    Article  Google Scholar 

  44. Lü J-h, Li H-k, An H-j, Wang G-h, Wang Y, Li M-q, et al. Positioning isolation and biochemical analysis of single DNA molecules based on Nanomanipulation and single-molecule PCR. J Am Chem Soc. 2004;126:11136–7.

    Article  Google Scholar 

  45. Hu J, Zhang Y, Gao H, Li M, Hartmann U. Artificial DNA patterns by mechanical Nanomanipulation. Nano Lett. 2002;2:55–7.

    Article  CAS  Google Scholar 

  46. Guthold M, Matthews G, Negishi A, Taylor RM, Erie D, Brooks FP, et al. Quantitative manipulation of DNA and viruses with the nanomanipulator scanning force microscope. Surf Interface Anal. 1999;27:437–43.

    Article  CAS  Google Scholar 

  47. Herman-Bausier P, Formosa-Dague C, Feuillie C, Valotteau C, Dufrêne YF. Forces guiding staphylococcal adhesion. J Struct Biol. 2017;197:65–9.

    Article  CAS  Google Scholar 

  48. Gould P. Lithography: rewriting the rules. Mater Today. 2003;6:34–9.

    CAS  Google Scholar 

  49. Li B, Zhang Y, Yan S-H, Lü J-H, Ye M, Li M-q, et al. Positioning scission of single DNA molecules with nonspecific endonuclease based on Nanomanipulation. J Am Chem Soc. 2007;129:6668–9.

    Article  CAS  Google Scholar 

  50. Neuman KC, Nagy A. Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy. Nat Methods. 2008;5:491–505.

    Article  CAS  Google Scholar 

  51. Hughes ML, Dougan L. The physics of pulling polyproteins: a review of single molecule force spectroscopy using the AFM to study protein unfolding. Rep Prog Phys. 2016;79:076601.

    Article  Google Scholar 

  52. Rief M, Oesterhelt F, Heymann B, Gaub HE. Single molecule force spectroscopy on polysaccharides by atomic force microscopy. Science. 1997;275:1295–7.

    Article  CAS  Google Scholar 

  53. Rief M, Clausen-Schaumann H, Gaub HE. Sequence-dependent mechanics of single DNA molecules. Nat Struct Biol. 1999;6:346–9.

    Article  CAS  Google Scholar 

  54. Formosa-Dague C, Speziale P, Foster TJ, Geoghegan JA, Dufrêne YF. Zinc-dependent mechanical properties of Staphylococcus aureus biofilm-forming surface protein SasG. Proc Natl Acad Sci. 2016;113:410–5.

    Article  CAS  Google Scholar 

  55. Eghiaian F, Rigato A, Scheuring S. Structural, mechanical, and dynamical variability of the actin cortex in living cells. Biophys J. 2015;108:1330–40.

    Article  CAS  Google Scholar 

  56. Alsteens D, Newton R, Schubert R, Martinez-Martin D, Delguste M, Roska B, et al. Nanomechanical mapping of first binding steps of a virus to animal cells. Nat Nanotechnol. 2017;12:177–83.

    Article  CAS  Google Scholar 

  57. Chacko JV, Canale C, Harke B, Diaspro A. Sub-diffraction Nano manipulation using STED AFM. PLoS One. 2013;8:e66608.

    Article  CAS  Google Scholar 

  58. Langelüddecke L, Singh P, Deckert V. Exploring the nanoscale: fifteen years of tip-enhanced Raman spectroscopy. Appl Spectrosc. 2015;69:1357–71.

    Article  Google Scholar 

  59. Zhang R, Zhang Y, Dong ZC, Jiang S, Zhang C, Chen LG, et al. Chemical mapping of a single molecule by plasmon-enhanced Raman scattering. Nature. 2013;498:82.

    Article  CAS  Google Scholar 

  60. Sadashivappa G, Sharvari N. Nanoantenna-a review. Int J Renew Energy Technol Res. 2015;4:1–9.

    Google Scholar 

  61. Patel SK, Argyropoulos C. Plasmonic nanoantennas: enhancing light-matter interactions at the nanoscale. EPJ Appl Metamater. 2015;2:4.

    Article  Google Scholar 

  62. Cohen M, Shavit R, Zalevsky Z. Observing optical Plasmons on a single nanometer scale. Sci Rep. 2014;4:4096.

    Article  Google Scholar 

  63. Bigourdan F, Hugonin J-P, Marquier F, Sauvan C, Greffet J-J. Nanoantenna for electrical generation of surface Plasmon Polaritons. Phys Rev Lett. 2016;116:106803.

    Article  Google Scholar 

  64. Chan CU, Ohl C-D. Total-internal-reflection-fluorescence microscopy for the study of Nanobubble dynamics. Phys Rev Lett. 2012;109:174501.

    Article  Google Scholar 

  65. Tan BH, An H, Ohl C-D. Resolving the pinning force of Nanobubbles with optical microscopy. Phys Rev Lett. 2017;118:054501.

    Article  Google Scholar 

  66. Dinç S. A simple and green extraction of carbon dots from sugar beet molasses: biosensor applications. Surg Industry. 2016;141:560–4.

    Google Scholar 

  67. Bennun SV, Faller R, Longo ML. Drying and rehydration of DLPC/DSPC symmetric and asymmetric supported lipid bilayers: a combined AFM and fluorescence microscopy study. Langmuir. 2008;24:10371–81.

    Article  CAS  Google Scholar 

  68. Koh CJ, Lee M. Structural analysis of amyloid aggregates by multifunctional fluorescence nanoscopy. Curr Appl Phys. 2006;6:e257–e60.

    Article  Google Scholar 

  69. Gradinaru CC, Martinsson P, Aartsma TJ, Schmidt T. Simultaneous atomic-force and two-photon fluorescence imaging of biological specimens in vivo. Ultramicroscopy. 2004;99:235–45.

    Article  CAS  Google Scholar 

  70. Pierini F, Zembrzycki K, Nakielski P, Pawłowska S, Kowalewski TA. Atomic force microscopy combined with optical tweezers (AFM/OT). Meas Sci Technol. 2016;27:025904.

    Article  Google Scholar 

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Acknowledgements

H. A. acknowledges the support from the National Key R&D Program of China (2016YFD0400800), and the National Natural Science Foundation of China (NSFC-31470090 and NSFC-31571029). H. Y. acknowledges the financial support by the Singapore Ministry of Education Academic Research Fund Tier 1 (R-143-000-A40-114) and project 31371851 supported by NSFC.

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Authors and Affiliations

  1. Food Science and Technology Programme, c/o Department of Chemistry, National University of Singapore, Singapore, Singapore

    Xiao Feng & Hongshun Yang

  2. JPK Instruments AG, Shanghai, China

    Yunchang Guo

  3. Division of Physics and Applied Physics, Nanyang Technological University Singapore, Singapore, Singapore

    Hongjie An

Authors
  1. Xiao Feng
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  2. Yunchang Guo
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  3. Hongjie An
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  4. Hongshun Yang
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Corresponding author

Correspondence to Hongjie An .

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Editors and Affiliations

  1. State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China

    Jiye Cai

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Feng, X., Guo, Y., An, H., Yang, H. (2018). The Hyphenated Technique of High Speed Atomic Force Microscopy and Super Resolution Optical Detection System. In: Cai, J. (eds) Atomic Force Microscopy in Molecular and Cell Biology. Springer, Singapore. https://doi.org/10.1007/978-981-13-1510-7_6

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