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ICTMI 2017 pp 151–161Cite as

Significance of MTA1 Expression Status in Progesterone Responsiveness of Endometrial Cancer Cells

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Abstract

Progesterone or progestin therapy is given to endometrial cancer (EC) patients when surgery and chemotherapy are not acceptable treatment modalities due to various reasons. The success rate of this therapy is quite often low mainly because of resistance and non-responsiveness in patients. The presence of progesterone receptors (PR) is necessary for progesterone (P4)-induced transcription but not sufficient. Along with the ligand–receptor binding, recruitment of transcriptional co-regulators is essential for induction of transcription. In our study, we have investigated whether the level of transcriptional coregulator, metastasis tumor antigen 1 (MTA1) level influences progesterone-mediated transcriptional regulation and P4 responsiveness in EC cells. Ishikawa cells in which MTA1 is over-expressed or silenced are treated with progesterone, and the gene expression profile of relevant genes involved in invasion, EMT, and drug resistance are studied by real-time PCR. Cells with high expression of MTA1 showed a gene expression profile similar to EMT signature and enhanced drug transporter expression. Progesterone treatment is also found to differentially regulate progesterone receptor expression in MTA1 over-expressed and silenced cells. To understand the mechanism of differential gene regulation by progesterone in MTA1 high/low cells, further analysis is required. Along with the PR status, MTA1 level is highly significant in determining the outcome of progesterone treatment of endometrial cancer cells.

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Acknowledgements

Chithra J. S. was financially supported by Grant No. 09/716(0318)/2011-EMR-1 from CSIR, Government of India.

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Authors and Affiliations

  1. Cancer Research Program-4, Rajiv Gandhi Centre for Biotechnology, Trivandrum, 695014, India

    J. S. Chithra & S. Asha Nair

Authors
  1. J. S. Chithra
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  2. S. Asha Nair
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Correspondence to S. Asha Nair .

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Editors and Affiliations

  1. Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore

    Balázs Gulyás

  2. Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore

    Parasuraman Padmanabhan

  3. Mar Ephraem College Of Engineering and Technology , Kanyakumari Dt, Tamil Nadu, India

    A. Lenin Fred

  4. Rajiv Gandhi Centre for Biotechnology , Thiruvananthapuram, Kerala, India

    T. R. Santhosh Kumar

  5. Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore

    Sundramurthy Kumar

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Chithra, J.S., Asha Nair, S. (2019). Significance of MTA1 Expression Status in Progesterone Responsiveness of Endometrial Cancer Cells. In: Gulyás, B., Padmanabhan, P., Fred, A., Kumar, T., Kumar, S. (eds) ICTMI 2017. Springer, Singapore. https://doi.org/10.1007/978-981-13-1477-3_12

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  • DOI: https://doi.org/10.1007/978-981-13-1477-3_12

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-13-1476-6

  • Online ISBN: 978-981-13-1477-3

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