Abstract
One advantage of nanodisc is the ability to control the size of the lipid bilayer, as the size of the nanodisc is monitored by the size of the membrane scaffold protein used during nanodisc assembly. In this chapter we utilized this property of nanodisc in order to investigate the effect of size of nanodisc on the function of membrane protein. E. coli expressed bacteriorhodopsin was used in this study to avoid residual native lipid, and a mutation was introduced to increase protein expression yield. The result suggested that the different sizes of nanodisc have a less significant influence over the photocycle kinetic compared to the effect due tot charge.
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Acknowledgements
The plasmid of wild type HmbRI and HmbRII, as well as E. coli C43(DE3) used for expressing HmbRID94N was generously provided by Professor Chii-Shen Yang, from the Department of Biochemical Science and Technology, National Taiwan University.
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Yeh, V. (2019). Effect of Size of Nanodisc. In: Study of Bacteriorhodopsin in a Controlled Lipid Environment. Springer Theses. Springer, Singapore. https://doi.org/10.1007/978-981-13-1238-0_4
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DOI: https://doi.org/10.1007/978-981-13-1238-0_4
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