Abstract
This chapter introduces the experimental techniques employed for assembly of nanodisc and functional studies of bacteriorhodopsin. The assembled nanodisc was purified using chromatographic methods, and the oligomeric state of bacteriorhodopsin was verified using visible wavelength circular dichroism. The function of bacteriorhodopsin, which is connected to its photocycle, was investigated using transient absorption spectroscopy and transient photocurrent measurements. Finally, protein production and purification processes are also presented in this chapter.
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Yeh, V. (2019). Experimental Background. In: Study of Bacteriorhodopsin in a Controlled Lipid Environment. Springer Theses. Springer, Singapore. https://doi.org/10.1007/978-981-13-1238-0_2
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DOI: https://doi.org/10.1007/978-981-13-1238-0_2
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