Impact of Three-Dimentional Culture Systems on Hepatic Differentiation of Puripotent Stem Cells and Beyond

  • Thamil Selvee RamasamyEmail author
  • Agnes Lee Chen Ong
  • Wei Cui
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1077)


Generation of functional hepatocytes from human pluripotent stem cells (hPSCs) is a vital tool to produce large amounts of human hepatocytes, which hold a great promise for biomedical and regenerative medicine applications. Despite a tremendous progress in developing the differentiation protocols recapitulating the developmental signalling and stages, these resulting hepatocytes from hPSCs yet achieve maturation and functionality comparable to those primary hepatocytes. The absence of 3D milieu in the culture and differentiation of these hepatocytes may account for this, at least partly, thus developing an optimal 3D culture could be a step forward to achieve this aim. Hence, review focuses on current development of 3D culture systems for hepatic differentiation and maturation and the future perspectives of its application.


Tissue engineering Regenerative medicine 3D culture Hepatocytes Pluripotent stem cells 



This work was supported by the University of Malaya Research Grant (UMRG RG465-12HTM) and Ministry of Education Malaysia, Fundamental Research Grant Scheme (FRGS FP044-2014B).


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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Thamil Selvee Ramasamy
    • 1
    • 2
    • 3
    Email author
  • Agnes Lee Chen Ong
    • 1
  • Wei Cui
    • 3
  1. 1.Stem Cell Biology Laboratory, Department of Molecular Medicine, Faculty of MedicineUniversity of MalayaKuala LumpurMalaysia
  2. 2.Cell & Molecular Biology Laboratory, The Dean’s Office, Faculty of MedicineUniversity of MalayaKuala LumpurMalaysia
  3. 3.Department of Surgery and Cancer, Institute of Reproductive and Developmental BiologyImperial College LondonLondonUK

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