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A Novel Strategy for Simple and Robust Expansion of Human Pluripotent Stem Cells Using Botulinum Hemagglutinin

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Novel Biomaterials for Regenerative Medicine

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1077))

Abstract

Clinical and industrial application of human pluripotent stem cells (hPSCs) has been hindered by the lack of robust strategies to sustain cultures in an undifferentiated state. Here, we describe a simple and robust method to culture and propagate hPSCs, which we anticipate will remove major roadblocks in investigating the basic properties of undifferentiated hPSCs and accelerate cell-based manufacturing. We also provide an overview of the use of botulinum hemagglutinin, an inhibitor of E-cadherin, to maintain and expand various hPSC lines in an undifferentiated state in different culture conditions. Hemagglutinin selectively removes cells that have lost the undifferentiated state, dissociates aggregates in situ, and is easy to use, scalable, and reproducible.

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Acknowledgments

This work was supported by “Development of cell manufacturing and processing system for industrialization of regenerative medicine” (No. P14006), a project commissioned by Japan Agency for Medical Research and Development.

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Authors and Affiliations

  1. Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan

    Mee-Hae Kim & Masahiro Kino-oka

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  1. Mee-Hae Kim
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  2. Masahiro Kino-oka
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Correspondence to Masahiro Kino-oka .

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Editors and Affiliations

  1. The Catholic University of Korea, Seoul, Korea (Republic of)

    Heung Jae Chun

  2. Korea Institute of Science and Technology, Seoul, Korea (Republic of)

    Kwideok Park

  3. Korea Institute of Radiological and Medical Sciences, Seoul, Korea (Republic of)

    Chun-Ho Kim

  4. Chonbuk National University, Jeonju, Korea (Republic of)

    Gilson Khang

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Kim, MH., Kino-oka, M. (2018). A Novel Strategy for Simple and Robust Expansion of Human Pluripotent Stem Cells Using Botulinum Hemagglutinin. In: Chun, H., Park, K., Kim, CH., Khang, G. (eds) Novel Biomaterials for Regenerative Medicine. Advances in Experimental Medicine and Biology, vol 1077. Springer, Singapore. https://doi.org/10.1007/978-981-13-0947-2_2

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