Abstract
Enzymes are chemical catalysts par excellence. Mechanistic understanding of enzyme catalysis therefore emphasizes two key features – the kinetic pathway and the chemical route taken to achieve it. Elucidation of kinetic mechanisms was elaborated in several chapters earlier (in Part III). Chemical tools and cofactor reactivity exploited by these catalysts formed the major focus of this section (Part IV). Assimilating the kinetic and the chemical lines of inquiry completes the comprehension of enzyme function. The origins of enzyme catalytic power (see Chap. 6) make better sense through this fusion. There may be multiple chemical solutions for catalyzing a particular reaction, for instance, peptide bond hydrolysis (Chap. 7). A chosen chemical mechanism may place constraints as to what kind of kinetic schemes are feasible. Besides, different kinetic mechanisms may exist for the same enzyme-catalyzed reaction. We will attempt to bring together these two broad lines of evidence in this chapter through examples.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Craik CC, Rocznaik S, Largman C, Rutter WJ (1987) The catalytic role of active site aspartic acid in serine proteases. Science 237:909–913
Duodoroff M, Barker HA, Hassid WZ (1947) Studies with bacterial sucrose phosphorylase. I. The mechanism of action of sucrose phosphorylase as a glucose-transferring enzyme (transglucosidase). J Biol Chem 168:725–732
Johnson CM, Roderick SL, Cook PF (2005) The serine acetyltransferase reaction: acetyl transfer from an acylpantothenyl donor to an alcohol. Arch Biochem Biophys 433:85–95
Prakash P, Punekar NS, Bhaumik P (2018) Structural basis for the catalytic mechanism and α-ketoglutarate cooperativity of glutamate dehydrogenase. J Biol Chem 293:6241–6258
Raines RT (1998) Ribonuclease A. Chem Rev 98:1045–1065
Samland AK, Sprenger GA (2006) Microbial aldolases as C–C bonding enzymes – unknown treasures and new developments. Appl Microbiol Biotechnol 71:253–264
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Punekar, N.S. (2018). Integrating Kinetic and Chemical Mechanisms: A Synthesis. In: ENZYMES: Catalysis, Kinetics and Mechanisms. Springer, Singapore. https://doi.org/10.1007/978-981-13-0785-0_36
Download citation
DOI: https://doi.org/10.1007/978-981-13-0785-0_36
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-13-0784-3
Online ISBN: 978-981-13-0785-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)