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Good Kinetic Practices

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ENZYMES: Catalysis, Kinetics and Mechanisms

Abstract

Generating reliable enzyme data requires clean experimental design and good kinetic practices. Certain practical considerations are important in this quest. This chapter will describe many such aspects of experimentation.

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References

  • Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RMM (1966) Hydrogen ion buffers for biological research. Biochemistry 5:467–477

    Article  CAS  Google Scholar 

  • Selwyn MJ (1965) A simple test for enzyme inactivation of an enzyme during assay. Biochim Biophys Acta 105:193–195

    Article  CAS  Google Scholar 

  • Tipton KF, Armstrong RN, Bakker BM, Bairoch A, Cornish-Bowden A, Halling PJ, Hofmeyr J-H, Leyh TS, Kettner C, Raushel FM, Rohwer J, Schomburg D, Steinbeck C (2014) Standards for reporting enzyme data: the STRENDA Consortium: what it aims to do and why it should be helpful. Perspect Sci 1:131–137

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Punekar, N.S. (2018). Good Kinetic Practices. In: ENZYMES: Catalysis, Kinetics and Mechanisms. Springer, Singapore. https://doi.org/10.1007/978-981-13-0785-0_13

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