Abstract
Neurospora crassa colonizes freshly burnt plant biomass and shows robust growth on cellulosic material. This model filamentous fungus has well-developed genetics, biochemistry, and molecular biology. A collection of resources and tools available for this organism makes it an ideal model system for a systems-level elucidation of the mechanisms underlying plant cell wall deconstruction by filamentous fungi. Research on N. crassa has contributed to the discovery of a new class of plant cell wall-degrading enzymes called lytic polysaccharide monooxygenases (LPMOs). The availability of a full genome deletion strain set for N. crassa has expedited the identification of two essential transcription factors, CLR-1 and CLR-2, which are required for the expression of cellulolytic genes and growth on crystalline cellulose. Recently, a detailed network in N. crassa was constructed of biochemical reactions important for the degradation of plant cell wall polysaccharides, providing a scaffold for a systems analysis of plant cell wall deconstruction. In this chapter, we cover recent progress toward elucidation of plant cell wall deconstruction by N. crassa. Remarkable recent progress provides new strategies for targeted strain improvement for the production of plant cell wall-degrading enzymes.
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Acknowledgments
This work was supported by the Science and Technology Support Program of 12th Five-Year Plan under grant No. 2015BAD15B0503, the Ministry of Science and Technology (MOST), China, and by the Start-up Research Grant, Northwest A&F University, Yangling, Shaanxi, China. S.C. dedicates this work to Professor David B. Wilson (deceased), in memory of his forty years contribution to the enzymology of cellulases.
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Chen, S. et al. (2018). The Model Filamentous Fungus Neurospora crassa: Progress Toward a Systems Understanding of Plant Cell Wall Deconstruction. In: Fang, X., Qu, Y. (eds) Fungal Cellulolytic Enzymes. Springer, Singapore. https://doi.org/10.1007/978-981-13-0749-2_6
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