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Fungal Cellulolytic Enzymes pp 223–238Cite as

Determination of Cellulase Activities and Model for Lignocellulose Saccharification

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Abstract

The hydrolytic ability of cellulase enzymes being used in the current lignocellulosic “biorefinery” industry has stimulated a huge motivation to make an efficient cellulase preparation. Increasing work has shown how important the role that some basic conceptions and cognitions can play in close relation with an effective enzymatic hydrolysis. To shed light on the lignocellulose hydrolysis behavior in this review, hydrolytic performance of cellulase enzyme on various cellulosic substrates existing in form of carboxymethylcellulose (CMC), Avicel, dissolving pulp, filter paper, and natural lignocellulose was reviewed. A development of defining the enzyme activity of cellulase preparation, including specific enzyme and total enzyme mixture, was considered. Finally, the prediction of activity and dynamics of cellulase enzymes was outlooked.

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Acknowledgment

The work was funded by the National Natural Science Foundation of China (21776114; 21176106) and China Postdoctoral Science Foundation (2016T90419). Part of the work was also supported by State Key Laboratory of Microbial Technology (M2016-12) and the Jiangsu Province “Six Talent Peak” (XNY-010). We also give the thanks to the Priority Academic Program Development of Jiangsu Higher Education Institutions, the 111 Project (No. 111-2-06), and the Jiangsu province “Collaborative Innovation Center for Advanced Industrial Fermentation” industry development program.

This chapter was modified in part from the paper published by our group in Enzyme and Microbial Technology (Sun et al., 2015; 79: 42–48). The related contents are reused with permission.

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Authors and Affiliations

  1. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China

    Fubao Sun, Marie Rose Mukasekuru & Danyang Chen

  2. Jinan City Institute for Food and Drug Control, Jinan, China

    Yongtao Wei & Xiaohui Lin

  3. State Key Laboratory of Microbial Technology, Shandong University, Jinan, China

    Lijuan Han & Xu Fang

Authors
  1. Fubao Sun
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  2. Marie Rose Mukasekuru
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  3. Danyang Chen
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  4. Yongtao Wei
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  5. Lijuan Han
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  6. Xiaohui Lin
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  7. Xu Fang
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Correspondence to Fubao Sun .

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Editors and Affiliations

  1. State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong, China

    Xu Fang

  2. State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong, China

    Yinbo Qu

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Sun, F. et al. (2018). Determination of Cellulase Activities and Model for Lignocellulose Saccharification. In: Fang, X., Qu, Y. (eds) Fungal Cellulolytic Enzymes. Springer, Singapore. https://doi.org/10.1007/978-981-13-0749-2_12

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