Abstract
Lignocellulose is the most abundantly sustainable raw material on the earth. To increase the utilization of sustainable lignocellulosic biomass, yeast, especially Saccharomyces cerevisiae, is used as a microbial cell factory to produce renewable chemicals like ethanol, butanol, or other fermentative products from lignocellulosic biomass. The cost of cellulolytic enzymes including cellulase and hemi cellulase is one of the limitations for industrial applications. Combining the cellulolytic enzyme expression and chemical production is a promising strategy to improve cost efficiency. Although cellulolytic enzyme-encoding genes have been expressed in yeast for enzyme characterization and protein engineering, co-expression of multiple cellulolytic enzyme genes is required to enable the non-cellulolytic yeast to utilize lignocellulose. Types of co-expressed systems include free cellulolytic enzyme, surface-displayed enzyme, or artificial minicellulosome. However, a lignocellulosic biomass utilizing strain with practical applications has been not constructed so far. The low efficiency of cellulolytic enzyme expression is the major roadblock hampering the generation of the desired strain. Although strong promoter, multiple gene copies, and codon optimization improve the gene expression, the protein misfolding, glycosylation, and vesicle transportation during secretion are more critical. Co-expression of multiple cellulolytic enzymes in an optimal ratio is also a challenging in cellulolytic yeast construction. Other than S. cerevisiae, nonconventional yeasts such as Kluyveromyces marxianus and Yarrowia lipolytica with special properties such as thermotolerance, xylose utilization, and lipid production are also good candidates for lignocellulosic biomass utilizing microbial cell factory construction.
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References
Adrio JL (2017) Oleaginous yeasts: promising platforms for the production of oleochemicals and biofuels. Biotechnol Bioeng 114(9):1915–1920. https://doi.org/10.1002/bit.26337
Bae J, Kuroda K, Ueda M (2015) Proximity effect among cellulose-degrading enzymes displayed on the Saccharomyces cerevisiae. Appl Environ Microbiol 81(1):59–66. https://doi.org/10.1128/Aem.02864-14
Banat IM, Marchant R (1995) Characterization and potential industrial applications of 5 novel thermotolerant fermentative yeast strains. World J Microbiol Biotechnol 11(3):304–306
Banat IM, Singh D, Marchant R (1996) The use of a thermotolerant fermentative Kluyveromyces marxianus IMB3 yeast strain for ethanol production. Acta Biotechnol 16(2–3):215–223
Brethauer S, Studer MH (2015) Biochemical conversion processes of lignocellulosic biomass to fuels and chemicals – a review. Chimia (Aarau) 69(10):572–581. https://doi.org/10.2533/chimia.2015.572
Chang JJ, Ho CY, Ho FJ, Tsai TY, Ke HM, Wang CHT, Chen HL, Shih MC, Huang CC, Li WH (2012) PGASO: a synthetic biology tool for engineering a cellulolytic yeast. Biotechnol Biofuels 5:53. https://doi.org/10.1186/1754-6834-5-53
Chang JJ, Ho FJ, Ho CY, Wu YC, Hou YH, Huang CC, Shih MC, Li WH (2013) Assembling a cellulase cocktail and a cellodextrin transporter into a yeast host for CBP ethanol production. Biotechnol Biofuels 6(1):19. https://doi.org/10.1186/1754-6834-6-19
Chen Y, Xiao W, Wang Y, Liu H, Li X, Yuan Y (2016) Lycopene overproduction in Saccharomyces cerevisiae through combining pathway engineering with host engineering. Microb Cell Factories 15(113):113. https://doi.org/10.1186/s12934-016-0509-4
Cheng YS, Chen CC, Huang JW, Ko TP, Huang ZY, Guo RT (2015) Improving the catalytic performance of a GH11 xylanase by rational protein engineering. Appl Microbiol Biotechnol 99(22):9503–9510. https://doi.org/10.1007/s00253-015-6712-0
Cherry JR, Fidantsef AL (2003) Directed evolution of industrial enzymes: an update. Curr Opin Biotechnol 14(4):438–443. https://doi.org/10.1016/S0958-1669(03)00099-5
Cho KM, Yoo YJ, Kang HS (1999) Delta-integration of endo/exo-glucanase and beta-glucosidase genes into the yeast chromosomes for direct conversion of cellulose to ethanol. Enzyme Microb Technol 25(1-2):23–30. https://doi.org/10.1016/S0141-0229(99)00011-3
Davison SA, den Haan R, van Zyl WH (2016) Heterologous expression of cellulase genes in natural Saccharomyces cerevisiae strains. Appl Microbiol Biotechnol 100(18):8241–8254. https://doi.org/10.1007/s00253-016-7735-x
Davy AM, Kildegaard HF, Andersen MR (2017) Cell factory engineering. Cell Sys 4(3):262–275. https://doi.org/10.1016/j.cels.2017.02.010
den Haan R, Mcbride JE, La Grange DC, Lynd LR, Van Zyl WH (2007a) Functional expression of cellobiohydrolases in Saccharomyces cerevisiae towards one-step conversion of cellulose to ethanol. Enzyme Microb Technol 40(5):1291–1299. https://doi.org/10.1016/j.enzmictec.2006.09.022
den Haan R, Rose SH, Lynd LR, van Zyl WH (2007b) Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae. Metab Eng 9(1):87–94. https://doi.org/10.1016/j.ymben.2006.08.005
den Haan R, Kroukamp H, van Zyl JHD, van Zyl WH (2013) Cellobiohydrolase secretion by yeast: current state and prospects for improvement. Process Biochem 48(1):1–12. https://doi.org/10.1016/j.procbio.2012.11.015
den Haan R, van Rensburg E, Rose SH, Gorgens JF, van Zyl WH (2015) Progress and challenges in the engineering of non-cellulolytic microorganisms for consolidated bioprocessing. Curr Opin Biotechnol 33:32–38. https://doi.org/10.1016/j.copbio.2014.10.003
Duquesne S, Bozonnet S, Bordes F, Dumon C, Nicaud JM, Marty A (2014) Construction of a highly active xylanase displaying oleaginous yeast: comparison of anchoring systems. PLoS One 9(4):e95128. https://doi.org/10.1371/journal.pone.0095128
Ergun BG, Calik P (2016) Lignocellulose degrading extremozymes produced by Pichia pastoris: current status and future prospects. Bioprocess Biosyst Eng 39(1):1–36. https://doi.org/10.1007/s00449-015-1476-6
Fan LH, Zhang ZJ, Yu XY, Xue YX, Tan TW (2012) Self-surface assembly of cellulosomes with two miniscaffoldins on Saccharomyces cerevisiae for cellulosic ethanol production. Proc Natl Acad Sci USA 109(33):13260–13265. https://doi.org/10.1073/pnas.1209856109
Fan LH, Zhang ZJ, Mei S, Lu YY, Li M, Wang ZY, Yang JG, Yang ST, Tan TW (2016) Engineering yeast with bifunctional minicellulosome and cellodextrin pathway for co-utilization of cellulose-mixed sugars. Biotechnol Biofuels 9:137. https://doi.org/10.1186/s13068-016-0554-6
Fitzpatrick J, Kricka W, James TC, Bond U (2014) Expression of three Trichoderma reesei cellulase genes in Saccharomyces pastorianus for the development of a two-step process of hydrolysis and fermentation of cellulose. J Appl Microbiol 117(1):96–108. https://doi.org/10.1111/jam.12494
Fujita Y, Takahashi S, Ueda M, Tanaka A, Okada H, Morikawa Y, Kawaguchi T, Arai M, Fukuda H, Kondo A (2002) Direct and efficient production of ethanol from cellulosic material with a yeast strain displaying cellulolytic enzymes. Appl Environ Microbiol 68(10):5136–5141. https://doi.org/10.1128/Aem.68.10.5136-5141.2002
Fujita Y, Ito J, Ueda M, Fukuda H, Kondo A (2004) Synergistic saccharification, and direct fermentation to ethanol, of amorphous cellulose by use of an engineered yeast strain codisplaying three types of cellulolytic enzyme. Appl Environ Microbiol 70(2):1207–1212
Gao SL, Tong YY, Zhu L, Ge M, Zhang YA, Chen DJ, Jiang Y, Yang S (2017) Iterative integration of multiple-copy pathway genes in Yarrowia lipolytica for heterologous beta-carotene production. Metab Eng 41:192–201. https://doi.org/10.1016/j.ymben.2017.04.004
Gong YX, Tang GY, Wang MM, Li JB, Xiao WJ, Lin JH, Liu ZH (2014) Direct fermentation of amorphous cellulose to ethanol by engineered Saccharomyces cerevisiae coexpressing Trichoderma viride EG3 and BGL1. J Gen Appl Microbiol 60(5):198–206. https://doi.org/10.2323/jgam.60.198
Goyal G, Tsai SL, Madan B, DaSilva NA, Chen W (2011) Simultaneous cell growth and ethanol production from cellulose by an engineered yeast consortium displaying a functional mini-cellulosome. Microb Cell Factories 10(89):89. https://doi.org/10.1186/1475-2859-10-89
Greene ER, Himmel ME, Beckham GT, Tan ZP (2015) Glycosylation of Cellulases: engineering better enzymes for biofuels. Adv Carbohydr Chem Biochem 72:63–112. https://doi.org/10.1016/bs.accb.2015.08.001
Hong KK, Nielsen J (2012) Metabolic engineering of Saccharomyces cerevisiae: a key cell factory platform for future biorefineries. Cell Mol Life Sci 69(16):2671–2690. https://doi.org/10.1007/s00018-012-0945-1
Hong J, Tamaki H, Akiba S, Yamamoto K, Kumagai H (2001) Cloning of a gene encoding a highly stable endo-beta-1,4-glucanase from Aspergillus niger and its expression in yeast. J Biosci Bioeng 92(5):434–441. https://doi.org/10.1263/jbb.92.434
Hong J, Tamaki H, Yamamoto K, Kumagai H (2003a) Cloning of a gene encoding a thermo-stable endo-beta-1,4-glucanase from Thermoascus aurantiacus and its expression in yeast. Biotechnol Lett 25(8):657–661. https://doi.org/10.1023/A:1023072311980
Hong J, Tamaki H, Yamamoto K, Kumagai H (2003b) Cloning of a gene encoding thermostable cellobiohydrolase from Thermoascus aurantiacus and its expression in yeast. Appl Microbiol Biotechnol 63(1):42–50. https://doi.org/10.1007/s00253-003-1379-3
Hong J, Wang Y, Kumagai H, Tamaki H (2007) Construction of thermotolerant yeast expressing thermostable cellulase genes. J Biotechnol 130(2):114–123. https://doi.org/10.1016/j.jbiotec.2007.03.008
Ilmen M, den Haan R, Brevnova E, McBride J, Wiswall E, Froehlich A, Koivula A, Voutilainen SP, Siika-Aho M, la Grange DC, Thorngren N, Ahlgren S, Mellon M, Deleault K, Rajgarhia V, van Zyl WH, Penttila M (2011) High level secretion of cellobiohydrolases by Saccharomyces cerevisiae. Biotechnol Biofuels 4:30. https://doi.org/10.1186/1754-6834-4-30
Inokuma K, Hasunuma T, Kondo A (2014) Efficient yeast cell-surface display of exo- and endo-cellulase using the SED1 anchoring region and its original promoter. Biotechnol Biofuels 7:8. https://doi.org/10.1186/1754-6834-7-8
Inokuma K, Bamba T, Ishii J, Ito Y, Hasunuma T, Kondo A (2016) Enhanced cell-surface display and secretory production of cellulolytic enzymes with Saccharomyces cerevisiae Sed1 signal peptide. Biotechnol Bioeng 113(11):2358–2366. https://doi.org/10.1002/bit.26008
Katahira S, Fujita Y, Mizuike A, Fukuda H, Kondo A (2004) Construction of a xylan-fermenting yeast strain through codisplay of xylanolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells. Appl Environ Microbiol 70(9):5407–5414. https://doi.org/10.1128/Aem.70.9.5407-5414.2004
Kavscek M, Strazar M, Curk T, Natter K, Petrovic U (2015) Yeast as a cell factory: current state and perspectives. Microb Cell Factories 14:94. https://doi.org/10.1186/s12934-015-0281-x
Kim SY, Sohn JH, Pyun YR, Choi ES (2007) Variations in protein glycosylation in Hansenula polymorpha depending on cell culture stage. J Mol Microbiol Biotechnol 17(12):1949–1954
Kim S, Baek SH, Lee K, Hahn JS (2013) Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and beta-glucosidase. Microb Cell Fact 12:14. https://doi.org/10.1186/1475-2859-12-14
Kim HM, Jung S, Lee KH, Song Y, Bae HJ (2015) Improving lignocellulose degradation using xylanase-cellulase fusion protein with a glycine-serine linker. Int J Biol Macromol 73:215–221. https://doi.org/10.1016/j.ijbiomac.2014.11.025
Klein T, Niklas J, Heinzle E (2015) Engineering the supply chain for protein production/secretion in yeasts and mammalian cells. J Ind Microbiol Biotechnol 42(3):453–464. https://doi.org/10.1007/s10295-014-1569-2
Kricka W, Fitzpatrick J, Bond U (2014) Metabolic engineering of yeasts by heterologous enzyme production for degradation of cellulose and hemicellulose from biomass: a perspective. Front Microbiol 5:174. https://doi.org/10.3389/fmicb.2014.00174
Lambertz C, Garvey M, Klinger J, Heesel D, Klose H, Fischer R, Commandeur U (2014) Challenges and advances in the heterologous expression of cellulolytic enzymes: a review. Biotechnol Biofuels 7:135. https://doi.org/10.1186/s13068-014-0135-5
Lane S, Zhang S, Wei N, Rao C, Jin YS (2015) Development and physiological characterization of cellobiose-consuming Yarrowia lipolytica. Biotechnol Bioeng 112(5):1012–1022. https://doi.org/10.1002/bit.25499
Larue K, Melgar M, Martin VJJ (2016) Directed evolution of a fungal beta-glucosidase in Saccharomyces cerevisiae. Biotechnol Biofuels 9:52. https://doi.org/10.1186/s13068-016-0470-9
Li MJ, Kildegaard KR, Chen Y, Rodriguez A, Borodina I, Nielsen J (2015) De novo production of resveratrol from glucose or ethanol by engineered Saccharomyces cerevisiae. Metab Eng 32:1–11. https://doi.org/10.1016/j.ymben.2015.08.007
Liu Z, Inokuma K, Ho SH, den Haan R, Hasunuma T, van Zyl WH, Kondo A (2015) Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae. Biotechnol Biofuels 8:162. https://doi.org/10.1186/s13068-015-0344-6
Lynd LR, van Zyl WH, McBride JE, Laser M (2005) Consolidated bioprocessing of cellulosic biomass: an update. Curr Opin Biotechnol 16(5):577–583
Matano Y, Hasunuma T, Kondo A (2012) Display of cellulases on the cell surface of Saccharomyces cerevisiae for high yield ethanol production from high-solid lignocellulosic biomass. Bioresour Technol 108:128–133. https://doi.org/10.1016/j.biortech.2011.12.144
Matano Y, Hasunuma T, Kondo A (2013a) Cell recycle batch fermentation of high-solid lignocellulose using a recombinant cellulase-displaying yeast strain for high yield ethanol production in consolidated bioprocessing. Bioresour Technol 135:403–409. https://doi.org/10.1016/j.biortech.2012.07.025
Matano Y, Hasunuma T, Kondo A (2013b) Simultaneous improvement of saccharification and ethanol production from crystalline cellulose by alleviation of irreversible adsorption of cellulase with a cell surface-engineered yeast strain. Appl Microbiol Biotechnol 97(5):2231–2237. https://doi.org/10.1007/s00253-012-4587-x
Mellitzer A, Weis R, Glieder A, Flicker K (2012) Expression of lignocellulolytic enzymes in Pichia pastoris. Microb Cell Factories 11(61):61. https://doi.org/10.1186/1475-2859-11-61
Muller S, Sandal T, Kamp-Hansen P, Dalboge H (1998) Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Yarrowia lipolytica. Yeast 14(14):1267–1283
Nakatani Y, Yamada R, Ogino C, Kondo A (2013) Synergetic effect of yeast cell-surface expression of cellulase and expansin-like protein on direct ethanol production from cellulose. Microb Cell Fact 12:66. https://doi.org/10.1186/1475-2859-12-66
Njokweni AP, Rose SH, van Zyl WH (2012) Fungal beta-glucosidase expression in Saccharomyces cerevisiae. J Ind Microbiol Biotechnol 39(10):1445–1452. https://doi.org/10.1007/s10295-012-1150-9
Olson DG, McBride JE, Shaw AJ, Lynd LR (2012) Recent progress in consolidated bioprocessing. Curr Opin Biotechnol 23(3):396–405. https://doi.org/10.1016/j.copbio.2011.11.026
Paddon CJ, Westfall PJ, Pitera DJ, Benjamin K, Fisher K, McPhee D, Leavell MD, Tai A, Main A, Eng D, Polichuk DR, Teoh KH, Reed DW, Treynor T, Lenihan J, Fleck M, Bajad S, Dang G, Dengrove D, Diola D, Dorin G, Ellens KW, Fickes S, Galazzo J, Gaucher SP, Geistlinger T, Henry R, Hepp M, Horning T, Iqbal T, Jiang H, Kizer L, Lieu B, Melis D, Moss N, Regentin R, Secrest S, Tsuruta H, Vazquez R, Westblade LF, Xu L, Yu M, Zhang Y, Zhao L, Lievense J, Covello PS, Keasling JD, Reiling KK, Renninger NS, Newman JD (2013) High-level semi-synthetic production of the potent antimalarial artemisinin. Nature 496(7446):528–532. https://doi.org/10.1038/nature12051
Park CS, Chang CC, Ryu DD (2000) Expression and high-level secretion of Trichoderma reesei endoglucanase I in Yarrowia lipolytica. Appl Biochem Biotechnol 87(1):1–15
Resch MG, Donohoe BS, Baker JO, Decker SR, Bayer EA, Beckham GT, Himmel ME (2013) Fungal cellulases and complexed cellulosomal enzymes exhibit synergistic mechanisms in cellulose deconstruction. Energy Environ Sci 6(6):1858–1867. https://doi.org/10.1039/c3ee00019b
Schroder M (2008) Engineering eukaryotic protein factories. Biotechnol Lett 30(2):187–196. https://doi.org/10.1007/s10529-007-9524-1
Song HT, Yang YM, Liu DK, Xu XQ, Xiao WJ, Liu ZL, Xia WC, Wang CY, Yu X, Jiang ZB (2017) Construction of recombinant Yarrowia lipolytica and its application in bio-transformation of lignocellulose. Bioengineered 8(5):624–629. https://doi.org/10.1080/21655979.2017.1293219
Sun J, Wen F, Si T, Xu JH, Zhao HM (2012) Direct conversion of xylan to ethanol by recombinant Saccharomyces cerevisiae strains displaying an engineered minihemicellulosome. Appl Environ Microbiol 78(11):3837–3845. https://doi.org/10.1128/Aem.07679-11
Suzuki H, Imaeda T, Kitagawa T, Kohda K (2012) Deglycosylation of cellulosomal enzyme enhances cellulosome assembly in Saccharomyces cerevisiae. J Biotechnol 157(1):64–70. https://doi.org/10.1016/j.jbiotec.2011.11.015
Tang H, Song M, He Y, Wang J, Wang S, Shen Y, Hou J, Bao X (2017) Engineering vesicle trafficking improves the extracellular activity and surface display efficiency of cellulases in Saccharomyces cerevisiae. Biotechnol Biofuels 10:53. https://doi.org/10.1186/s13068-017-0738-8
Tsai SL, Oh J, Singh S, Chen RZ, Chen W (2009) Functional assembly of minicellulosomes on the Saccharomyces cerevisiae cell surface for cellulose hydrolysis and ethanol production. Appl Environ Microbiol 75(19):6087–6093. https://doi.org/10.1128/Aem.01538-09
Tsai SL, Goyal G, Chen W (2010) Surface display of a functional Minicellulosome by intracellular complementation using a synthetic yeast consortium and its application to cellulose hydrolysis and ethanol production. Appl Environ Microbiol 76(22):7514–7520. https://doi.org/10.1128/Aem.01777-10
Ueda M, Tanaka A (2000) Cell surface engineering of yeast: construction of arming yeast with biocatalyst. J Biosci Bioeng 90(2):125–136. https://doi.org/10.1016/S1389-1723(00)80099-7
Van Rensburg P, Van Zyl WH, Pretorius IS (1998) Engineering yeast for efficient cellulose degradation. Yeast 14(1):67–76. https://doi.org/10.1002/(Sici)1097-0061(19980115)14:1<67::Aid-Yea200>3.0.Co;2-T
van Zyl WH, Lynd LR, den Haan R, McBride JE (2007) Consolidated bioprocessing for bioethanol production using Saccharomyces cereviside. Adv Biochem Eng Biotechnol 108:205–235. https://doi.org/10.1007/10_2007_061
Van Zyl JHD, Den Haan R, Van Zyl WH (2014) Over-expression of native Saccharomyces cerevisiae exocytic SNARE genes increased heterologous cellulase secretion. Appl Microbiol Biotechnol 98(12):5567–5578. https://doi.org/10.1007/s00253-014-5647-1
Van Zyl J, Den Haan R, Van Zyl W (2016) Overexpression of native Saccharomyces cerevisiae ER-to-Golgi SNARE genes increased heterologous cellulase secretion. Appl Microbiol Biotechnol 100(1):505–518. https://doi.org/10.1007/s00253-015-7022-2
Voutilainen SP, Murray PG, Tuohy MG, Koivula A (2010) Expression of Talaromyces emersonii cellobiohydrolase Cel7A in Saccharomyces cerevisiae and rational mutagenesis to improve its thermostability and activity. Protein Eng Des Sel 23(2):69–79. https://doi.org/10.1093/protein/gzp072
Voutilainen SP, Nurmi-Rantala S, Penttila M, Koivula A (2014) Engineering chimeric thermostable GH7 cellobiohydrolases in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 98(7):2991–3001. https://doi.org/10.1007/s00253-013-5177-2
Wei H, Wang W, Alahuhta M, Vander Wall T, Baker JO, Taylor LE 2nd, Decker SR, Himmel ME, Zhang M (2014) Engineering towards a complete heterologous cellulase secretome in Yarrowia lipolytica reveals its potential for consolidated bioprocessing. Biotechnol Biofuels 7(1):148. https://doi.org/10.1186/s13068-014-0148-0
Wen F, Sun J, Zhao HM (2010) Yeast surface display of trifunctional Minicellulosomes for simultaneous Saccharification and fermentation of cellulose to ethanol. Appl Environ Microbiol 76(4):1251–1260. https://doi.org/10.1128/Aem.01687-09
Xu LL, Shen Y, Hou J, Peng BY, Tang HT, Bao XM (2014) Secretory pathway engineering enhances secretion of cellobiohydrolase I from Trichoderma reesei in Saccharomyces cerevisiae. J Biosci Bioeng 117(1):45–52. https://doi.org/10.1016/j.jbiosc.2013.06.017
Yamada R, Taniguchi N, Tanaka T, Ogino C, Fukuda H, Kondo A (2010) Cocktail delta-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains. Microb Cell Fact 9:32. https://doi.org/10.1186/1475-2859-9-32
Yamada R, Taniguchi N, Tanaka T, Ogino C, Fukuda H, Kondo A (2011) Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression. Biotechnol Biofuels 4:8. https://doi.org/10.1186/1754-6834-4-8
Yamada R, Hasunuma T, Kondo A (2013) Endowing non-cellulolytic microorganisms with cellulolytic activity aiming for consolidated bioprocessing. Biotechnol Adv 31(6):754–763. https://doi.org/10.1016/j.biotechadv.2013.02.007
Yanase S, Hasunuma T, Yamada R, Tanaka T, Ogino C, Fukuda H, Kondo A (2010a) Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes. Appl Microbiol Biotechnol 88(1):381–388. https://doi.org/10.1007/s00253-010-2784-z
Yanase S, Yamada R, Kaneko S, Noda H, Hasunuma T, Tanaka T, Ogino C, Fukuda H, Kondo A (2010b) Ethanol production from cellulosic materials using cellulase-expressing yeast. Biotechnol J 5(5):449–455. https://doi.org/10.1002/biot.200900291
Yang PZ, Zhang HF, Jiang ST (2016) Construction of recombinant sestc Saccharomyces cerevisiae for consolidated bioprocessing, cellulase characterization, and ethanol production by in situ fermentation. 3 Biotech 6:192. https://doi.org/10.1007/s13205-016-0512-9
Zhang B, Zhang J, Wang DM, Han RX, Ding R, Gao XL, Sun LH, Hong J (2016) Simultaneous fermentation of glucose and xylose at elevated temperatures co-produces ethanol and xylitol through overexpression of a xylose-specific transporter in engineered Kluyveromyces marxianus. Bioresour Technol 216:227–237. https://doi.org/10.1016/j.biortech.2016.05.068
Zhang B, Zhu Y, Zhang J, Wang D, Sun L, Hong J (2017) Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose. Bioresour Technol 224:553–562. https://doi.org/10.1016/j.biortech.2016.11.110
Zhu H, Yao SD, Wang SL (2010) MF alpha signal peptide enhances the expression of Cellulase eg1 gene in yeast. Appl Biochem Biotechnol 162(3):617–624. https://doi.org/10.1007/s12010-009-8880-9
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Wang, D., Hong, J. (2018). Expression of Cellulolytic Enzymes in Yeast. In: Fang, X., Qu, Y. (eds) Fungal Cellulolytic Enzymes. Springer, Singapore. https://doi.org/10.1007/978-981-13-0749-2_11
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