Abstract
The importance of RNA-protein interactions in regulation of mRNA and non-coding RNA function is increasingly appreciated. With the development of next generation high-throughput sequencing technologies, a variety of methods have been proposed to comprehensively identify RNA-protein interactions. In this chapter, we discussed the traditional and state-of-the-art technologies that were used to study RNA-protein interaction, including experimental and computational methods. To help highlight the biological significance of RNA-protein interaction in complex diseases, online resources on RNA-protein interactions were briefly discussed. Finally, we discussed the interaction among noncoding RNAs (such as long noncoding RNAs and microRNAs) and proteins, as well as the dysregulation of RNA-protein interaction in complex diseases. These summarization will ultimately provide a more complete picture for understanding of the function of RNA-protein interactions, including how these interaction assembled and how they modulate cellular function in complex diseases.
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Xu, J., Wang, Z., Jin, X., Li, L., Pan, T. (2018). Methods for Identification of Protein-RNA Interaction. In: Li, X., Xu, J., Xiao, Y., Ning, S., Zhang, Y. (eds) Non-coding RNAs in Complex Diseases. Advances in Experimental Medicine and Biology, vol 1094. Springer, Singapore. https://doi.org/10.1007/978-981-13-0719-5_12
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DOI: https://doi.org/10.1007/978-981-13-0719-5_12
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