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Therapeutic Antibody Discovery in Infectious Diseases Using Single-Cell Analysis

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Single Cell Biomedicine

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1068))

Abstract

Since the discovery of mouse hybridoma technology by Kohler and Milstein in 1975, significant progress has been made in monoclonal antibody production. Advances in B cell immortalization and phage display technologies have generated a myriad of valuable monoclonal antibodies for diagnosis and treatment. Technological breakthroughs in various fields of ‘omics have shed crucial insights into cellular heterogeneity of a biological system in which the functional individuality of a single cell must be considered. Based on this important concept, remarkable discoveries in single-cell analysis have made in identifying and isolating functional B cells that produce beneficial therapeutic monoclonal antibodies. In this review, we will discuss three traditional methods of antibody discovery. Recent technological platforms for single-cell antibody discovery will be reviewed. We will discuss the application of the single-cell analysis in finding therapeutic antibodies for human immunodeficiency virus and emerging Zika arbovirus.

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Acknowledgements

This work was supported by the Florida Department of Health, Biomedical Research Program (JKY, CQN) and NIAID 1R21AI130561-01A1 (CQN).

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Authors and Affiliations

  1. Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA

    Alexandria Voigt, Touyana Semenova, Janet Yamamoto, Veronique Etienne & Cuong Q. Nguyen

  2. Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, USA

    Cuong Q. Nguyen

  3. Center of Orphaned Autoimmune Diseases, University of Florida, Gainesville, FL, USA

    Cuong Q. Nguyen

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  1. Alexandria Voigt
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  2. Touyana Semenova
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  3. Janet Yamamoto
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Correspondence to Cuong Q. Nguyen .

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Editors and Affiliations

  1. Henan Provincial People’s Hospital, Zhengzhou, Henan, China

    Jianqin Gu

  2. Shanghai Institute of Clinical Bioinformatics, Zhongshan Hospital, Fudan University, Shanghai, China

    Xiangdong Wang

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Voigt, A., Semenova, T., Yamamoto, J., Etienne, V., Nguyen, C.Q. (2018). Therapeutic Antibody Discovery in Infectious Diseases Using Single-Cell Analysis. In: Gu, J., Wang, X. (eds) Single Cell Biomedicine. Advances in Experimental Medicine and Biology, vol 1068. Springer, Singapore. https://doi.org/10.1007/978-981-13-0502-3_8

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