Abstract
To create tissue replacements with qualities similar to human tissues, and for ease of tissue loss repair, novel 3D printing fabrication methods have recently been introduced and popularized in the field of tissue engineering and regenerative medicine as an alternative to the scaffold fabrication methods. 3D printing may provide the fabricate process to better mimic the internal microstructure and external appearance. Printable bioink should be developed for stable 3D structure stratification. Advanced bioinks for 3D printing are rationally designed materials intended to improve the functionality of printed tissue scaffolds. The search for an appropriate bioink capable of providing a suitable microenvironment to support cellular activities is ongoing. The extracellular matrix (ECM) provides instructive cues for cell attachment, proliferation, differentiation, and ultimately tissue regeneration. The use of ECM-based biomaterials in regenerative medicine is therefore, rapidly expanding. In this respect, the decellularized ECM biomaterials have gained popularity as an excellent source of bioink, given its capability to inherit the intrinsic cues from a native ECM. In this chapter, we describe the current status of ECM-based biomaterials, the emerging trends in ECM bioink development, and bioink requirements that could enable proper selection of the bioink to fabricate an engineered tissue/organ. In particular, rheological properties of bioprinting materials are significant for printing resolution and shape fidelity. We propose a general method of measuring non-Newtonian rheological properties based on rotational rheometers in oscillatory mode. In addition, the mathematical modeling incorporating the power law model is discussed. These approaches can be easily used to optimize printing parameters and verify the bioink printability because a variety of dECM-based bioinks possess shear-thinning properties.
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References
Amini AR, Laurencin CT, Nukavarapu SP (2012) Bone tissue engineering: recent advances and challenges. Crit Rev Biomed Eng 40(5):363–408
Arealis G, Nikolaou VS (2015) Bone printing: new frontiers in the treatment of bone defects. Injury 46(Suppl 8):S20–S22. https://doi.org/10.1016/S0020-1383(15)30050-4
Atala A, Yoo JJ (2015) Essentials of 3D biofabrication and translation. Academic Press, London
Atala A, Kasper FK, Mikos AG (2012) Engineering complex tissues. Sci Transl Med 4(160):160rv112. https://doi.org/10.1126/scitranslmed.3004890
Attalla R, Ling C, Selvaganapathy P (2016) Fabrication and characterization of gels with integrated channels using 3D printing with microfluidic nozzle for tissue engineering applications. Biomed Microdevices 18(1):17. https://doi.org/10.1007/s10544-016-0042-6
Bajaj P, Schweller RM, Khademhosseini A, West JL, Bashir R (2014) 3D biofabrication strategies for tissue engineering and regenerative medicine. Annu Rev Biomed Eng 16:247–276. https://doi.org/10.1146/annurev-bioeng-071813-105155
Benders KE, van Weeren PR, Badylak SF, Saris DB, Dhert WJ, Malda J (2013) Extracellular matrix scaffolds for cartilage and bone regeneration. Trends Biotechnol 31(3):169–176
Bernfield M, Sanderson RD (1990) Syndecan, a developmentally regulated cell surface proteoglycan that binds extracellular matrix and growth factors. Philos Trans R Soc Lond Ser B Biol Sci 327(1239):171–186
Buyukhatipoglu K, Jo W, Sun W, Clyne AM (2009) The role of printing parameters and scaffold biopolymer properties in the efficacy of a new hybrid nano-bioprinting system. Biofabrication 1(3):035003
Buyukhatipoglu K, Chang R, Sun W, Clyne AM (2010) Bioprinted nanoparticles for tissue engineering applications. Tissue Eng Part C Methods 16(4):631–642. https://doi.org/10.1089/ten.TEC.2009.0280
Chan B, Leong K (2008) Scaffolding in tissue engineering: general approaches and tissue-specific considerations. Eur Spine J 17(4):467–479
Chen RN, Ho HO, Tsai YT, Sheu MT (2004) Process development of an acellular dermal matrix (ADM) for biomedical applications. Biomaterials 25(13):2679–2686
Chen C, Wang L, Deng L, Hu R, Dong A (2013) Performance optimization of injectable chitosan hydrogel by combining physical and chemical triple crosslinking structure. J Biomed Mater Res A 101(3):684–693. https://doi.org/10.1002/jbm.a.34364
Chimene D, Lennox KK, Kaunas RR, Gaharwar AK (2016) Advanced Bioinks for 3D Printing: A Materials Science Perspective. Ann Biomed Eng 44(6):2090–2102. https://doi.org/10.1007/s10439-016-1638-y
Combellack EJ, Jessop ZM, Naderi N, Griffin M, Dobbs T, Ibrahim A, Evans S, Burnell S, Doak SH, Whitaker IS (2016) Adipose regeneration and implications for breast reconstruction: update and the future. Gland Surg 5(2):227
Costantini M, Idaszek J, Szoke K, Jaroszewicz J, Dentini M, Barbetta A, Brinchmann JE, Swieszkowski W (2016) 3D bioprinting of BM-MSCs-loaded ECM biomimetic hydrogels for in vitro neocartilage formation. Biofabrication 8(3):035002. https://doi.org/10.1088/1758-5090/8/3/035002
Cui X, Boland T (2009) Human microvasculature fabrication using thermal inkjet printing technology. Biomaterials 30(31):6221–6227. https://doi.org/10.1016/j.biomaterials.2009.07.056
Das S, Pati F, Choi YJ, Rijal G, Shim JH, Kim SW, Ray AR, Cho DW, Ghosh S (2015) Bioprintable, cell-laden silk fibroin-gelatin hydrogel supporting multilineage differentiation of stem cells for fabrication of three-dimensional tissue constructs. Acta Biomater 11:233–246. https://doi.org/10.1016/j.actbio.2014.09.023
Delpech B, Vannier JP, Girard N, Bizet M, Delpech A, Lenormand B, Tilly H, Piguet H (1993) Expression of the hyaluronan-binding glycoprotein hyaluronectin in leukemias. Leukemia 7(2):172–176
Do AV, Khorsand B, Geary SM, Salem AK (2015) 3D printing of scaffolds for tissue regeneration applications. Adv Healthc Mater 4(12):1742–1762. https://doi.org/10.1002/adhm.201500168
Duan B, Hockaday LA, Kang KH, Butcher JT (2013) 3D bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogels. J Biomed Mater Res A 101(5):1255–1264. https://doi.org/10.1002/jbm.a.34420
Esposito M, Grusovin MG, Felice P, Karatzopoulos G, Worthington HV, Coulthard P (2009) The efficacy of horizontal and vertical bone augmentation procedures for dental implants – a Cochrane systematic review. Eur J Oral Implantol 2(3):167–184
Fedorovich NE, De Wijn JR, Verbout AJ, Alblas J, Dhert WJ (2008) Three-dimensional fiber deposition of cell-laden, viable, patterned constructs for bone tissue printing. Tissue Eng Part A 14(1):127–133. https://doi.org/10.1089/ten.a.2007.0158
Fedorovich NE, Alblas J, Hennink WE, Oner FC, Dhert WJ (2011) Organ printing: the future of bone regeneration? Trends Biotechnol 29(12):601–606. https://doi.org/10.1016/j.tibtech.2011.07.001
Floren M, Migliaresi C, Motta A (2016) Processing techniques and applications of silk hydrogels in bioengineering. J Funct Biomater 7(3):26
Freytes DO, Martin J, Velankar SS, Lee AS, Badylak SF (2008) Preparation and rheological characterization of a gel form of the porcine urinary bladder matrix. Biomaterials 29(11):1630–1637. https://doi.org/10.1016/j.biomaterials.2007.12.014
Gilbert TW, Sellaro TL, Badylak SF (2006) Decellularization of tissues and organs. Biomaterials 27(19):3675–3683. https://doi.org/10.1016/j.biomaterials.2006.02.014
Gopal S, Multhaupt HAB, Pocock R, Couchman JR (2017) Cell-extracellular matrix and cell-cell adhesion are linked by syndecan-4. Matrix Biol 60-61:57–69. https://doi.org/10.1016/j.matbio.2016.10.006
Gruene M, Pflaum M, Hess C, Diamantouros S, Schlie S, Deiwick A, Koch L, Wilhelmi M, Jockenhoevel S, Haverich A, Chichkov B (2011) Laser printing of three-dimensional multicellular arrays for studies of cell-cell and cell-environment interactions. Tissue Eng Part C Methods 17(10):973–982. https://doi.org/10.1089/ten.TEC.2011.0185
Gu Q, Hao J, Lu Y, Wang L, Wallace GG, Zhou Q (2015) Three-dimensional bio-printing. Sci China Life Sci 58(5):411–419. https://doi.org/10.1007/s11427-015-4850-3
Hennink WE, van Nostrum CF (2002) Novel crosslinking methods to design hydrogels. Adv Drug Deliv Rev 54(1):13–36
Highley CB, Prestwich GD, Burdick JA (2016) Recent advances in hyaluronic acid hydrogels for biomedical applications. Curr Opin Biotechnol 40:35–40. https://doi.org/10.1016/j.copbio.2016.02.008
Hoshiba T, Chen G, Endo C, Maruyama H, Wakui M, Nemoto E, Kawazoe N, Tanaka M (2016) Decellularized extracellular matrix as an in vitro model to study the comprehensive roles of the ECM in stem cell differentiation. Stem Cells Int 2016:6397820. https://doi.org/10.1155/2016/6397820
Hospodiuk M, Dey M, Sosnoski D, Ozbolat IT (2017) The bioink: a comprehensive review on bioprintable materials. Biotechnol Adv 35(2):217–239. https://doi.org/10.1016/j.biotechadv.2016.12.006
Jacobsson KG, Lindahl U (1987) Degradation of heparin proteoglycan in cultured mouse mastocytoma cells. Biochem J 246(2):409–415
Jang J, Kim TG, Kim BS, Kim SW, Kwon SM, Cho DW (2016) Tailoring mechanical properties of decellularized extracellular matrix bioink by vitamin B2-induced photo-crosslinking. Acta Biomater 33:88–95. https://doi.org/10.1016/j.actbio.2016.01.013
Ji S, Guvendiren M (2017) Recent advances in bioink design for 3D bioprinting of tissues and organs. Front Bioeng Biotechnol 5:23. https://doi.org/10.3389/fbioe.2017.00023
Jia J, Richards DJ, Pollard S, Tan Y, Rodriguez J, Visconti RP, Trusk TC, Yost MJ, Yao H, Markwald RR, Mei Y (2014) Engineering alginate as bioink for bioprinting. Acta Biomater 10(10):4323–4331. https://doi.org/10.1016/j.actbio.2014.06.034
Jones FS, Jones PL (2000) The tenascin family of ECM glycoproteins: structure, function, and regulation during embryonic development and tissue remodeling. Dev Dyn 218(2):235–259. https://doi.org/10.1002/(SICI)1097-0177(200006)218:2<235::AID-DVDY2>3.0.CO;2-G
Jung JP, Bhuiyan DB, Ogle BM (2016) Solid organ fabrication: comparison of decellularization to 3D bioprinting. Biomater Res 20(1):27. https://doi.org/10.1186/s40824-016-0074-2
Karasaki S (1980) [An adhesive glycoprotein polymer on cell surface: fibronectin (author’s transl)]. Tanpakushitsu Kakusan Koso 25(9):890–905
Khalil S, Sun W (2007) Biopolymer deposition for freeform fabrication of hydrogel tissue constructs. Mater Sci Eng C 27(3):469–478. https://doi.org/10.1016/j.msec.2006.05.023
Kim JH, Yoo JJ, Lee SJ (2016) Three-dimensional cell-based bioprinting for soft tissue regeneration. Tissue Eng Regen Med 6(13):647–662
Kim BS, Kim H, Gao G, Jang J, Cho DW (2017) Decellularized extracellular matrix: a step towards the next generation source for bioink manufacturing. Biofabrication 9(3):034104. https://doi.org/10.1088/1758-5090/aa7e98
Kreimendahl F, Kopf M, Thiebes AL, Duarte Campos DF, Blaeser A, Schmitz-Rode T, Apel C, Jockenhoevel S, Fischer H (2017) Three-dimensional printing and angiogenesis: tailored agarose-type I collagen blends comprise three-dimensional printability and angiogenesis potential for tissue-engineered substitutes. Tissue Eng Part C Methods 23(10):604–615. https://doi.org/10.1089/ten.TEC.2017.0234
Kyle S, Jessop ZM, Al-Sabah A, Whitaker IS (2017) ‘Printability’ of candidate biomaterials for extrusion based 3D printing: state-of-the-art. Adv Healthc Mater 6(16). https://doi.org/10.1002/adhm.201700264
Langer R, Vacanti J (2016) Advances in tissue engineering. J Pediatr Surg 51(1):8–12. https://doi.org/10.1016/j.jpedsurg.2015.10.022
Lazarev YA, Lobachov VM, Grishkovski BA, Shibnev VA, Grechishko VS, Finogenova MP, Esipova NG, Rogulenkova VN (1978) Formation of the collagen-like triple-helical structure in oligopeptides during elongation of the molecular chain. Biopolymers 17(5):1215–1233. https://doi.org/10.1002/bip.1978.360170509
Lee M, Wu BM (2012) Recent advances in 3D printing of tissue engineering scaffolds. Methods Mol Biol 868:257–267. https://doi.org/10.1007/978-1-61779-764-4_15
Lee HJ, Kim YB, Ahn SH, Lee JS, Jang CH, Yoon H, Chun W, Kim GH (2015) A new approach for fabricating collagen/ECM-based bioinks using Preosteoblasts and human adipose stem cells. Adv Healthc Mater 4(9):1359–1368. https://doi.org/10.1002/adhm.201500193
Lee H, Han W, Kim H, Ha DH, Jang J, Kim BS, Cho DW (2017) Development of liver Decellularized extracellular matrix bioink for three-dimensional cell printing-based liver tissue engineering. Biomacromolecules 18(4):1229–1237. https://doi.org/10.1021/acs.biomac.6b01908
Li H, Liu S, Lin L (2016) Rheological study on 3D printability of alginate hydrogel and effect of graphene oxide. Int J Bioprinting 2(2):54–66. https://doi.org/10.18063/ijb.2016.02.007
Longo UG, Loppini M, Forriol F, Romeo G, Maffulli N, Denaro V (2012) Advances in meniscal tissue engineering. Stem Cells Int 2012:420346. https://doi.org/10.1155/2012/420346
Malkin AY, Isayev AI (2017) Rheology: concepts, methods, and applications. Elsevier, Toronto
Mallikarjunaiah CA. A method to fabricate solid free form scaffolds for liver tissue engineering by using 3D printing: Kaunas University of Technology
Mandrycky C, Wang Z, Kim K, Kim DH (2016) 3D bioprinting for engineering complex tissues. Biotechnol Adv 34(4):422–434. https://doi.org/10.1016/j.biotechadv.2015.12.011
Markstedt K, Mantas A, Tournier I, Martinez Avila H, Hagg D, Gatenholm P (2015) 3D bioprinting human chondrocytes with Nanocellulose-alginate bioink for cartilage tissue engineering applications. Biomacromolecules 16(5):1489–1496. https://doi.org/10.1021/acs.biomac.5b00188
Mikos AG, Herring SW, Ochareon P, Elisseeff J, Lu HH, Kandel R, Schoen FJ, Toner M, Mooney D, Atala A, Van Dyke ME, Kaplan D, Vunjak-Novakovic G (2006) Engineering complex tissues. Tissue Eng 12(12):3307–3339. https://doi.org/10.1089/ten.2006.12.3307
Mironov V, Kasyanov V, Markwald RR (2011) Organ printing: from bioprinter to organ biofabrication line. Curr Opin Biotechnol 22(5):667–673. https://doi.org/10.1016/j.copbio.2011.02.006
Morrison FA (2001) Understanding rheology. Topics in chemical engineering. Oxford University Press, Oxford
Murphy SV, Atala A (2014) 3D bioprinting of tissues and organs. Nat Biotechnol 32(8):773–785
Nastase MV, Young MF, Schaefer L (2012) Biglycan: a multivalent proteoglycan providing structure and signals. J Histochem Cytochem 60(12):963–975. https://doi.org/10.1369/0022155412456380
Oberpenning F, Meng J, Yoo JJ, Atala A (1999) De novo reconstitution of a functional mammalian urinary bladder by tissue engineering. Nat Biotechnol 17(2):149–155. https://doi.org/10.1038/6146
Ott HC, Matthiesen TS, Goh SK, Black LD, Kren SM, Netoff TI, Taylor DA (2008) Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart. Nat Med 14(2):213–221. https://doi.org/10.1038/nm1684
Ozbolat IT (2016) 3D bioprinting: fundamentals, principles and applications. Academic Press, London
Ozbolat IT, Hospodiuk M (2016) Current advances and future perspectives in extrusion-based bioprinting. Biomaterials 76:321–343. https://doi.org/10.1016/j.biomaterials.2015.10.076
Ozbolat IT, Yu Y (2013) Bioprinting toward organ fabrication: challenges and future trends. IEEE Trans Biomed Eng 60(3):691–699. https://doi.org/10.1109/TBME.2013.2243912
Park SH, Park SR, Chung SI, Pai KS, Min BH (2005) Tissue-engineered cartilage using fibrin/hyaluronan composite gel and its in vivo implantation. Artif Organs 29(10):838–845. https://doi.org/10.1111/j.1525-1594.2005.00137.x
Park S-H, Jung CS, Min B-H (2016) Advances in three-dimensional bioprinting for hard tissue engineering. Tissue Eng Regen Med 13(6):622–635
Pati F, Jang J, Ha DH, Won Kim S, Rhie JW, Shim JH, Kim DH, Cho DW (2014) Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink. Nat Commun 5:3935. https://doi.org/10.1038/ncomms4935
Petri JB, Rott O, Wetzig T, Herrmann K, Haustein UF (1999) The small proteoglycan fibromodulin is expressed in mitotic, but not in postmitotic fibroblasts. Mol Cell Biol Res Commun 1(1):59–65. https://doi.org/10.1006/mcbr.1999.0113
Ren X, Wang F, Chen C, Gong X, Yin L, Yang L (2016) Engineering zonal cartilage through bioprinting collagen type II hydrogel constructs with biomimetic chondrocyte density gradient. BMC Musculoskelet Disord 17:301. https://doi.org/10.1186/s12891-016-1130-8
Rosso F, Giordano A, Barbarisi M, Barbarisi A (2004) From cell-ECM interactions to tissue engineering. J Cell Physiol 199(2):174–180. https://doi.org/10.1002/jcp.10471
Rutz AL, Hyland KE, Jakus AE, Burghardt WR, Shah RN (2015) A multimaterial bioink method for 3D printing tunable, cell-compatible hydrogels. Adv Mater 27(9):1607–1614. https://doi.org/10.1002/adma.201405076
Sahiner N, Sagbas S, Aktas N (2015) Single step natural poly(tannic acid) particle preparation as multitalented biomaterial. Mater Sci Eng C Mater Biol Appl 49:824–834. https://doi.org/10.1016/j.msec.2015.01.076
Scarritt ME, Pashos NC, Bunnell BA (2015) A review of cellularization strategies for tissue engineering of whole organs. Front Bioeng Biotechnol 3:43. https://doi.org/10.3389/fbioe.2015.00043
Schmidt CE, Baier JM (2000) Acellular vascular tissues: natural biomaterials for tissue repair and tissue engineering. Biomaterials 21(22):2215–2231
Schultheiss D, Gabouev AI, Cebotari S, Tudorache I, Walles T, Schlote N, Wefer J, Kaufmann PM, Haverich A, Jonas U, Stief CG, Mertsching H (2005) Biological vascularized matrix for bladder tissue engineering: matrix preparation, reseeding technique and short-term implantation in a porcine model. J Urol 173(1):276–280. https://doi.org/10.1097/01.ju.0000145882.80339.18
Sears NA, Seshadri DR, Dhavalikar PS, Cosgriff-Hernandez E (2016) A review of three-dimensional printing in tissue engineering. Tissue Eng Part B Rev 22(4):298–310. https://doi.org/10.1089/ten.TEB.2015.0464
Skardal A, Devarasetty M, Kang HW, Mead I, Bishop C, Shupe T, Lee SJ, Jackson J, Yoo J, Soker S, Atala A (2015) A hydrogel bioink toolkit for mimicking native tissue biochemical and mechanical properties in bioprinted tissue constructs. Acta Biomater 25:24–34. https://doi.org/10.1016/j.actbio.2015.07.030
Smith CM, Stone AL, Parkhill RL, Stewart RL, Simpkins MW, Kachurin AM, Warren WL, Williams SK (2004) Three-dimensional bioassembly tool for generating viable tissue-engineered constructs. Tissue Eng 10(9–10):1566–1576. https://doi.org/10.1089/ten.2004.10.1566
Sobral JM, Caridade SG, Sousa RA, Mano JF, Reis RL (2011) Three-dimensional plotted scaffolds with controlled pore size gradients: effect of scaffold geometry on mechanical performance and cell seeding efficiency. Acta Biomater 7(3):1009–1018
Song JJ, Ott HC (2011) Organ engineering based on decellularized matrix scaffolds. Trends Mol Med 17(8):424–432. https://doi.org/10.1016/j.molmed.2011.03.005
Song BR, Yang SS, Jin H, Lee SH, Park DY, Lee J, Park SR, Park SH, Min BH (2015) Three dimensional plotted extracellular matrix scaffolds using a rapid prototyping for tissue engineering application. Tissue Eng Regen Med 12(3):172–180
Stanton MM, Samitier J, Sanchez S (2015) Bioprinting of 3D hydrogels. Lab Chip 15(15):3111–3115. https://doi.org/10.1039/c5lc90069g
Suntornnond R, Tan EYS, An J, Chua CK (2016) A mathematical model on the resolution of extrusion bioprinting for the development of new bioinks. Materials (Basel) 9(9):756. https://doi.org/10.3390/ma9090756
Temenoff JS, Mikos AG (2000) Review: tissue engineering for regeneration of articular cartilage. Biomaterials 21(5):431–440
Trachtenberg JE, Placone JK, Smith BT, Fisher JP, Mikos AG (2017) Extrusion-based 3D printing of poly(propylene fumarate) scaffolds with hydroxyapatite gradients. J Biomater Sci Polym Ed 28(6):532–554. https://doi.org/10.1080/09205063.2017.1286184
Vozzi G, Previti A, De Rossi D, Ahluwalia A (2002) Microsyringe-based deposition of two-dimensional and three-dimensional polymer scaffolds with a well-defined geometry for application to tissue engineering. Tissue Eng 8(6):1089–1098
Wolf MT, Daly KA, Brennan-Pierce EP, Johnson SA, Carruthers CA, D'Amore A, Nagarkar SP, Velankar SS, Badylak SF (2012) A hydrogel derived from decellularized dermal extracellular matrix. Biomaterials 33(29):7028–7038. https://doi.org/10.1016/j.biomaterials.2012.06.051
Wong M, Lawton T, Goetinck PF, Kuhn JL, Goldstein SA, Bonadio J (1992) Aggrecan core protein is expressed in membranous bone of the chick embryo. Molecular and biomechanical studies of normal and nanomelia embryos. J Biol Chem 267(8):5592–5598
Xavier JR, Thakur T, Desai P, Jaiswal MK, Sears N, Cosgriff-Hernandez E, Kaunas R, Gaharwar AK (2015) Bioactive nanoengineered hydrogels for bone tissue engineering: a growth-factor-free approach. ACS Nano 9(3):3109–3118. https://doi.org/10.1021/nn507488s
Xiong JY, Narayanan J, Liu XY, Chong TK, Chen SB, Chung TS (2005) Topology evolution and gelation mechanism of agarose gel. J Phys Chem B 109(12):5638–5643. https://doi.org/10.1021/jp044473u
Xu Y, Wang X (2015) Fluid and cell behaviors along a 3D printed alginate/gelatin/fibrin channel. Biotechnol Bioeng 112(8):1683–1695. https://doi.org/10.1002/bit.25579
Xu T, Binder KW, Albanna MZ, Dice D, Zhao W, Yoo JJ, Atala A (2013) Hybrid printing of mechanically and biologically improved constructs for cartilage tissue engineering applications. Biofabrication 5(1):015001. https://doi.org/10.1088/1758-5082/5/1/015001
Yang Q, Peng J, Guo Q, Huang J, Zhang L, Yao J, Yang F, Wang S, Xu W, Wang A (2008) A cartilage ECM-derived 3-D porous acellular matrix scaffold for in vivo cartilage tissue engineering with PKH26-labeled chondrogenic bone marrow-derived mesenchymal stem cells. Biomaterials 29(15):2378–2387
Yeong WY, Chua CK, Leong KF, Chandrasekaran M (2004) Rapid prototyping in tissue engineering: challenges and potential. Trends Biotechnol 22(12):643–652. https://doi.org/10.1016/j.tibtech.2004.10.004
Yue B (2014) Biology of the extracellular matrix: an overview. J Glaucoma 23(8 Suppl 1):S20–S23. https://doi.org/10.1097/IJG.0000000000000108
Zhang YS, Yue K, Aleman J, Mollazadeh-Moghaddam K, Bakht SM, Yang J, Jia W, Dell'Erba V, Assawes P, Shin SR, Dokmeci MR, Oklu R, Khademhosseini A (2016) 3D bioprinting for tissue and organ fabrication. Ann Biomed Eng 45:148. https://doi.org/10.1007/s10439-016-1612-8
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This work was funded by a Marine Biotechnology Program grant (20150220) in the Ministry of Oceans and Fisheries, Korea and supported by the Pukyong National University Research Fund in 2016 (CD20161157).
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Nam, S.Y., Park, SH. (2018). ECM Based Bioink for Tissue Mimetic 3D Bioprinting. In: Noh, I. (eds) Biomimetic Medical Materials. Advances in Experimental Medicine and Biology, vol 1064. Springer, Singapore. https://doi.org/10.1007/978-981-13-0445-3_20
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