Microarray Chips (in Vitro Diagnosis)

  • Yoshihiro ItoEmail author


Photochemical methods have enabled the preparation of microarrays through photolithography. The microarray system was developed from multiple analysis thorough spatially addressable parallel chemical synthesis of peptides. Later his technology has been expanded from gene analysis to proteome analysis to various omics analyses. On the other hand, photo-immobilization has been used for the immobilization of various materials on one substrate by means of photo-induced radical reaction, extending from protein immobilization to cell and virus immobilizations. Multiple analyses are believed to contribute to the development of novel clinical diagnoses.


Microarray Screening Gene analysis Proteome Photolithography Photo-immobilization 


  1. 1.
    Fodor, S.P., Read, J.L., Pirrung, M.C., Stryer, L., Lu, A.T., Solas, D.: Light-directed, spatially addressable parallel chemical synthesis. Science 251, 767–773 (1991)CrossRefPubMedGoogle Scholar
  2. 2.
    Schena, M., Shalon, D., Davis, R.W., Brown, P.O.: Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270, 467–470 (1995)CrossRefPubMedGoogle Scholar
  3. 3.
    Moore, C.D., Ajala, O.Z., Zhu, H.: Applications in high-content functional protein microarrays. Curr. Opin. Chem. Biol. 30, 21–27 (2016)CrossRefPubMedGoogle Scholar
  4. 4.
    Yandell, K.: Getting your sugar fix, a guide to glycan microarrays. The Scientist, Apr, 2015
  5. 5.
    Liang, S., Xu, W., Horiuchi, K.Y., Wang, Y., Ma, H.: Chemical microarrays: a new tool for discovery enzyme inhibitors. Methods Mol. Biol. 572, 149–160 (2009)CrossRefPubMedGoogle Scholar
  6. 6.
    Coyle, R., Jia, J., Mei, Y.: Polymer microarray technology for stem cell engineering. Acta Biomater. 34, 60–72 (2016)CrossRefPubMedGoogle Scholar
  7. 7.
    Sun, H., Chen, G.Y.J., Yao, S.O.: Recent advances in microarray technologies for proteomics. Chem. Biol. 20, 685–699 (2013)CrossRefPubMedGoogle Scholar
  8. 8.
    Jonczyk, R., Kurth, T., Lavrentieva, A., Walter, J.-G., Scheper, T., Stahl, F.: Living cell microarrays: an overview of concepts. Microarrays 5, 11 (2016)CrossRefPubMedCentralGoogle Scholar
  9. 9.
    Hong, H.J., Koom, W.S., Koh, W.-G.: Cell microarray technologies for high-throughput cell-based biosensors. Sensors 17, 1293 (2017)CrossRefGoogle Scholar
  10. 10.
    Avninder, S., Ylaya, K., Hewitt, S.M.: Tissue microarray: a simple technology that has revolutionized research in pathology. J. Postgrad. Med. 54, 158–162 (2008)CrossRefPubMedGoogle Scholar
  11. 11.
    EQC/MAQC-III Consortium.: A comprehensive assessment of RNA-seq accuracy, reproducibility and information content by the Sequence Quality Control consortium. Nature Biotechnology 32, 903–914 (2014)Google Scholar
  12. 12.
    Ito, Y.: Photoimmobilization for microarrays. Biotechnol. Prog. 22, 924–932 (2006)CrossRefPubMedGoogle Scholar
  13. 13.
    Ito, Y., Moritsugu, N., Matsue, T., Mitsukoshi, K., Ayame, H., Ookouchi, N., Hattori, H., Tashiro, H., Sato, S., Ebisawa, M.: An automated multiplex specific IgE assay system using a photoimmobilized microarray. J. Biotechnol. 161, 414–421 (2012)CrossRefPubMedGoogle Scholar
  14. 14.
    Vigh-Conrad, K.A., Conrad, D.F., Preuss, D.: A protein allergen microarray detects specific IgE to pollen surface, cytoplasmic, and commercial allergen extracts. PLoS ONE 5, e10174 (2010)CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Fall, B.I., Eberlein-Konig, B., Behrendt, H., Niessner, R., Ring, J., Weller, M.G.: Microarrays for the screening of allergen-specific IgE in human serum. Anal. Chem. 75, 556–562 (2003)CrossRefPubMedGoogle Scholar
  16. 16.
    Matsudaira, T., Tsuzuki, S., Wada, A., Suwa, A., Kohsaka, H., Tomida, M., Ito, Y.: Automated microfluidic assay system for autoantibodies found in autoimmune diseases using a photoimmobilized autoantigen microarray. Biotechnol. Prog. 24, 1384–1392 (2008)CrossRefPubMedGoogle Scholar
  17. 17.
    Sivakumar, P.M., Moritsugu, N., Obuse, S., Isoshima, T., Tashiro, H., Ito, Y.: Novel microarrays for simultaneous serodiagnosis of multiple antiviral antibodies. PLoS ONE 8, e81726 (2013)CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Ito, Y., Nogawa, M., Takeda, M., Shibuya, T.: Photo-reactive polyvinylalcohol for photo-immobilized microarray. Biomaterials 26, 211–216 (2005)CrossRefPubMedGoogle Scholar
  19. 19.
    Belov, L., Huang, P., Barber, N., Mulligan, S.P., Christopherson, R.I.: Identification of repertoires of surface antigens on leukemias using an antibody microarray, Proteomics 3, 2147–2154 (2003)CrossRefPubMedGoogle Scholar
  20. 20.
    Miller, J.C., Zhou, H., Kwekel, J., Cavallo, R., Burke, J., Butler, E.B., Teh, B.S., Haab, B.B.: Antibody microarray profiling of human prostate cancer sera: antibody screening and identification of potential biomarkers, Proteomics 3, 56–63 (2003)CrossRefPubMedGoogle Scholar
  21. 21.
    Chevolot, Y., Martins, J., Milosevic, N., Léonard, D., Zeng, S., Malissard, M., Berger, E.G., Maier, P., Mathieu, H.J., Crout, D.H., Sigrist, H.: Immobilisation on polystyrene of diazirine derivatives of mono- and disaccharides: biological activities of modified surfaces. Bioorg. Med. Chem. 9, 2943–2953 (2001)CrossRefPubMedGoogle Scholar
  22. 22.
    Kanoh, N., Kumashiro, S., Simizu, S., Kondoh, Y., Hatakeyama, S., Tashiro, H., Osada, H.: Immobilization of natural products on glass slides by using a photoaffinity reaction and the detection of protein-small-molecule interactions. Angew. Chem. Int. Edn. 42, 5584–5587 (2003)CrossRefGoogle Scholar
  23. 23.
    Kondoh, Y., Honda, K., Osada Y.: Construction and application of a photo-cross-linked chemical array. Methods Mol. Biol. 1263, 29–41 (2015); In: Hempel, J.E., Williams, C.H., Hong, C.C. (eds.) Chemical Biology Methods and Protocols. Springer, New YorkGoogle Scholar
  24. 24.
    Weinrich, D., Köhn, M., Jonkheijm, P., Westerlind, U., Dehmelt, L., Engelkamp, H., Christianen, P.C.M., Kuhlmann, J., Maan, J.C., Nüsse, D., Schröder, H., Wacker, R., Voges, E., Breinbauer, R., Kunz, H., Niemeyer, C.M., Waldmann, H.: Preparation of biomolecule microstructures and microarrays by Thiol-ene photoimmobilization. ChemBioChem 11, 235–247 (2010)CrossRefPubMedGoogle Scholar
  25. 25.
    Knecht, B.G., Strasser, A., Dietrich, R., Märtlbauer, E., Niessner, R., Weller, M.G.: Automated microarray system for the simultaneous detection of antibiotics in milk. Anal. Chem. 76, 646–654 (2004)CrossRefPubMedGoogle Scholar
  26. 26.
    FitzGerald, S.P., Lamont, J.V., McConnell, R.I., Benchikh, E.O.: Development of a high-throughput automated analyzed using biochip array technology. Clin. Chem. 51, 1165–1176 (2005)CrossRefPubMedGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Nano Medical Engineering LaboratoryRIKENWakoJapan
  2. 2.Emergent Bioengineering Materials Research TeamRIKEN Center for Emergent Matter ScienceWakoJapan

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