Development of Innovative Bio-beam Using Microbial Induced Calcite Precipitation Technology

  • Changming Bu
  • Qian Dong
  • Kejun Wen
  • Lin Li
Conference paper


As a new environmental friendly and sustainable technique for soil improvement, microbial induced calcite precipitation (MICP) has been studied widely. Most of previous studies focus on soil modification. This study is to develop innovative bio-beams based on MICP technology. A modified permeable rigid mold has been developed to prepare the bio-beams specimens within the immersing method. The permeable mold provides supports for the bacteria to grow through fully contact cementation media and induce the calcium carbonate precipitated homogeneously within the pores of the sand to form the bio-beam. Bending tests have been conducted to evaluate the flexure behavior of MICP-treated bio-beams. Single and multiple treatment of MICP in the various cementation media concentrations have been conducted. Experimental results show that the vertical load of the MICP-treated bio-beams could reach to 1412 N when the bio-beams were treated for one time. The double treatments of the bio-beams increased to the 163% of bending strength of single-treatment. The peak bending strength of the triple MICP-treated bio-beam specimen increased to 200% of bending strength of single-treatment. The bending strength also increased with cementation media concentrations.


Bio-beam Microbial induced calcite precipitation Immersing method Bending strength 



This paper is based upon work supported by the National Science Foundation Grant No. 1531382 and U.S. Department of Transportation Grant No. (DTRT13-G-UTC50FHWA) through Maritime Transportation Research and Education Center. The first author acknowledges financial support from the China Scholarship Council under Grant No. 201408505113, from Chongqing Research Program of Basic Research and Frontier Technology No. cstc2014jcyjA30005, from Chongqing University of Science and Technology, No. CK2015Z27 and Chongqing BaYu Plan.


  1. 1.
    DeJong, J.T., Mortensenb, B.M., Martinez, B.C., Nelson, D.C.: Bio-mediated soil improvement. Ecol. Eng. 36(2), 197–210 (2010)CrossRefGoogle Scholar
  2. 2.
    Han, Z., Cheng, X., Ma, Q.: An experimental study on dynamic response for MICP strengthening liquefiable sands. Earthq. Eng. Eng. Vib. 15, 673 (2016). Scholar
  3. 3.
    Martinez, B.C., DeJong, J.T., Ginn, T.R., Montoya, B.M., Barkouki, T.H., Hunt, C., Tanyu, B., Major, D.: Experimental optimization of microbially-induced carbonate precipitation for soil improvement. J. Geotech. Geoenviron. Eng. 139(4), 587–598 (2013)CrossRefGoogle Scholar
  4. 4.
    Martin, D., Dodds, K., Ngwenya, B., Butler, I., Elphick, S.: Inhibition of Sporosarcina pasteurii under anoxic conditions: implications for subsurface carbonate precipitation and remediation via ureolysis. Environ. Sci. Technol. 46, 8351–8355 (2012)CrossRefGoogle Scholar
  5. 5.
    Whiffin, V., van Paassen, L., Harkes, M.: Microbial carbonate precipitation as a soil improvement technique. Geomicrobiol. J. 24, 1–7 (2007)CrossRefGoogle Scholar
  6. 6.
    Achal, V., Mukerjee, A., Reddy, M.S.: Biogenic treatment improves the durability and remediates the cracks of concrete structures. Constr. Build. Mater. 48, 1–5 (2013)CrossRefGoogle Scholar
  7. 7.
    DeJong, J.T., Soga, K., Kavazanjian, E., Burns, S., van Paassen, L., Al Qabany, A.: Biogeochemical processes and geotechnical applications: progress, opportunities, and challenges. Geotechnique 63(4), 287–301 (2013)CrossRefGoogle Scholar
  8. 8.
    Li, M., Li, L., Ogbonnaya, U., Wen, K., Tian, A., Amini, F.: Influence of fiber addition on mechanical properties of micp-treated sand. J. Mater. Civ. Eng. 28(4), 04015166 (2016)CrossRefGoogle Scholar
  9. 9.
    Wen, K., Li, L., Ogbonnaya, U., Li, Y., Bu, C., Liu, S., Li, C., Amini, F.: Development of an improved immersing method to enhance microbial induced calcite precipitation through multiple treatments in low cementation media concentration. Geotech. Geol. Eng. (in review)Google Scholar
  10. 10.
    Harkes, M.P., Booster, J.L., Paassen, L.A., Loosdrecht, M., Whiffin, V.S.: Microbial induced carbonate precipitation as ground improvement method – bacterial fixation and empirical correlation CaCO3 vs strength. In: International conference on BioGeoCivil Engineering, Delft, The Netherlands (2008)Google Scholar
  11. 11.
    Zhao, Q., Li, L., Li, C., Li, M., Zhang, H., Amini, F.: Factors effecting improvement of engineering properties of MICP-treated soil catalyzed by bacteria and urease. J. Mater. Civ. Eng. (2014a).
  12. 12.
    Bernardi, D., DeJong, J.T., Montoya, B.M., Martinez, B.C.: Bio-bricks: biologically cemented sandstone bricks. Constr. Build. Mater. 55, 462–469 (2014)CrossRefGoogle Scholar
  13. 13.
    Zhao, Q., Li, L., Li, C., Zhang, H., Amini, F.: A full contact flexible mold for preparing samples based on microbial induced calcite precipitation technology. Geotech. Test. J. 37(5), 1–5 (2014b)CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Chongqing University of Science and TechnologyChongqingChina
  2. 2.Jackson State UniversityJacksonUSA

Personalised recommendations