Abstract
In this work we develop DNA sensors that are based on charge switching in colour centres in diamond. The presented method allows the combination of luminescence sensor and electrochemical sensor working on the principle of electrochemical impedance spectroscopy (EIS). The sensor employs specifically designed diamond structures grown by the means of chemical Vapour deposition (CVD). This diamond structure consists of highly boron doped diamond electrode on which an intrinsic diamond layer is deposited. This intrinsic layer is about 15 nm thick and it contains NV colour centres. The device is then embedded in polydimethylsiloxane (PDMS) microfluidic flow cell and covered by a transparent indium tin oxide (ITO) coated electrode. The switching of the NV centre charge state as a response, on diamond surface termination, is crucial tool for the sensitive charged molecules sensing. First we demonstrated high sensitivity of the near surface NV centres on a diamond biosensor surface charge termination. The measured data are supported by band bending modelling. Negative O- terminated surface results in a preferable NV centre charge state of NV0 or NV−, whereas positive H- terminated surface leads to mostly non-PL NV+ charge state. By this principle any charged molecule, such as polymer on DNA, can be detected by a customized surface functionalization. Functionality of the microfluidic diamond device is also verified by the EIS.
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References
C. Schreyvogel, V. Polyakov, R. Wunderlich, J. Meijer and C. E. Nebel. Active charge state control of single NV centres in diamond by in-plane Al-Schottky junctions. Scientific Reports 5 (2015) 12160.
V. Petrakova, I. Rehor, J. Stursa, M. Ledvina, M. Nesladek, P. Cigler. Charge-sensitive fluorescent nanosensors created from nanodiamonds Nanoscale 7 (2015) 12307.
X. Chen, Ch. Zou, Z. Gong, Ch. Dong, G. Guo and F. Sun. Subdiffraction optical manipulation of the charge state of nitrogen vacancy center in diamond. Light: Science and Applications 4(1) (2015) e230.
S. Karavelia, O. Gaathona, A. Wolcotta, R. Sakakibaraa, O. A. Shemeshe, D. S. Peterkag, Edward S. Boydene, J. S. Owend, R. Yusteg and Dirk Englund. Modulation of nitrogen vacancy charge state and fluorescence in nanodiamonds using electrochemical potential. Proceedings of the National Academy of Sciences of the United States of America 113(15) (2016) 3938–3943.
V. Petrakova, V. Benson, M. Buncek, A. Fiserova, M. Ledvina, J. Stursa, P. Cigler and M. Nesladek. Imaging of transfection and intracellular release of intact, non-labeled DNA using fluorescent nanodiamonds. Nanoscale 8 (2016) 12002–12012.
V.D. Blank, V.N. Denisov, A.N. Kirichenko, M.S. Kuznetsov, B.N. Mavrin, S.A. Nosukhin, S.A. Terentiev. Raman scattering by defect-induced excitations in boron-doped diamond single crystals. Diamond & Related Materials 17(11) (2008) 1840–1843.
V. Petráková, M. Nesládek, A. Taylor, F. Fendrych, P. Cígler, M. Ledvina, J. Vacík, J. Štursa, and J. Kučka. Luminescence properties of engineered nitrogen vacancy centers in a close surface proximity. Phys. Status Solidi 208(9) (2011) 2051–2056.
R. F. Teófilo, H. J. Ceragioli, A. C. Peterlevitz, L. M. Da Silva, F. S. Damos, M. M. C. Ferreira, V. Baranauskas, L.T. Kubota. Improvement of the electrochemical properties of “as-grown” boron-doped polycrystalline diamond electrodes deposited on tungsten wires using ethanol. J Solid State Electrochem 11 (2007) 1449–1457.
B. P. Chaplina, D. K. Hublerb, J. Farrell. Understanding anodic wear at boron doped diamond film electrodes. Electrochimica Acta 89 (2013) 122–131.
Acknowledgements
The authors acknowledge the institutional resources of the Department of Biomedical Technology FBMI CTU; CTU grant SGS14/214/OHK4/3T/17; the Czech Science Foundation (GACR) Grant ID: GAČR 16-16336S; EU–FP7 research grant DIADEMS, No. 611143, FWO (Flanders) G.0.943.11.N.10.; the Erasmus Student Mobility Grant and the J.E. Purkyne fellowship awarded by Academy of Sciences of the Czech Republic.
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Krečmarová, M. et al. (2019). Microfluidic Diamond Biosensor Using NV Centre Charge State Detection. In: Lhotska, L., Sukupova, L., Lacković, I., Ibbott, G. (eds) World Congress on Medical Physics and Biomedical Engineering 2018. IFMBE Proceedings, vol 68/3. Springer, Singapore. https://doi.org/10.1007/978-981-10-9023-3_6
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DOI: https://doi.org/10.1007/978-981-10-9023-3_6
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