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Advanced Optical Methods for Brain Imaging pp 167–187Cite as

Advanced Miniature Microscopy for Brain Imaging

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Part of the book series: Progress in Optical Science and Photonics ((POSP,volume 5))

Abstract

To image neuronal activities down to single spines in freely behaving animal has already been the holy grail of neuroscientists. To achieve that goal, two-photon microscope must be miniaturized to be attached to the animal without interfering animal movements. In the past fifteen years, many groups have published different designs, albeit that none of them is not widely used by the neuroscience community. Here, we have summarized the major challenges that prevent prevalent applications of current miniature two-photon microscopy (TPM) for high-resolution imaging in freely behaving mice, and different configurations that may be used to address each challenge. Based on this theoretical analysis, we have provided detailed design of our high-resolution, miniaturized two-photon microscope (FHIRM-TPM) and its latest revisions that enable volumetric imaging capability and larger field of view and deeper penetration depth.

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Authors and Affiliations

  1. State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Peking University, Beijing, China

    Weijian Zong & Liangyi Chen

Authors
  1. Weijian Zong
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  2. Liangyi Chen
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Correspondence to Weijian Zong or Liangyi Chen .

Editor information

Editors and Affiliations

  1. Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan

    Fu-Jen Kao

  2. Department of Electrical and Computer Engineering, Boston University, Boston, Massachusetts, USA

    Gerd Keiser

  3. Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan

    Ankur Gogoi

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© 2019 Springer Nature Singapore Pte Ltd.

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Zong, W., Chen, L. (2019). Advanced Miniature Microscopy for Brain Imaging. In: Kao, FJ., Keiser, G., Gogoi, A. (eds) Advanced Optical Methods for Brain Imaging. Progress in Optical Science and Photonics, vol 5. Springer, Singapore. https://doi.org/10.1007/978-981-10-9020-2_9

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  • DOI: https://doi.org/10.1007/978-981-10-9020-2_9

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-10-9019-6

  • Online ISBN: 978-981-10-9020-2

  • eBook Packages: EngineeringEngineering (R0)

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